Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. Photoprotective and Cytotoxic Effects of the B. cordata Cell Culture Methanolic Extract on 3T3-Swiss albino Fibroblasts
2.2. The B. cordata Cell Culture Methanolic Extract Absorbs UV Light, Has a High Total Phenol Content, and Contains Bioactive SMs
3. Discussion
4. Materials and Methods
4.1. Preparation of the Methanolic Extract and Verbascoside Standard
4.2. In Vitro Bioassays
4.2.1. Conditions of the Cell Culture
4.2.2. Determination of the Photoprotective Activity of the Extract
Irradiation and Selection of the UVB Energy Dose
Photoprotective Bioassay
4.2.3. Determination of the Cytotoxicity of the Extract
4.2.4. Determination of Cell Viability
4.3. Phytochemical Analysis
4.3.1. Measurement of the UV Absorption Spectrum
4.3.2. Quantitative Analysis
4.3.3. Identification of Extract Secondary Metabolites through LC/MS
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gómez-Hernández, M.A.; Flores-Merino, M.V.; Sánchez-Flores, J.E.; Burrola-Aguilar, C.; Zepeda-Gómez, C.; Nieto-Trujillo, A.; Estrada-Zúñiga, M.E. Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts. Plants 2021, 10, 266. https://doi.org/10.3390/plants10020266
Gómez-Hernández MA, Flores-Merino MV, Sánchez-Flores JE, Burrola-Aguilar C, Zepeda-Gómez C, Nieto-Trujillo A, Estrada-Zúñiga ME. Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts. Plants. 2021; 10(2):266. https://doi.org/10.3390/plants10020266
Chicago/Turabian StyleGómez-Hernández, Milton Abraham, Miriam V. Flores-Merino, Jesús Enrique Sánchez-Flores, Cristina Burrola-Aguilar, Carmen Zepeda-Gómez, Aurelio Nieto-Trujillo, and María Elena Estrada-Zúñiga. 2021. "Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts" Plants 10, no. 2: 266. https://doi.org/10.3390/plants10020266