Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent
Abstract
:1. Introduction
2. Results and Discussion
2.1. Growth of C. sorokiniana AM-02 under Different Conditions
2.2. Bacterial Community Structure
3. Materials and Methods
3.1. Digestate-Based Media Preparation
3.2. Cultivation Conditions in a Photobioreactor
3.3. Analytical Methods
3.4. Bacterial Community Structure Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatment | Dry Weight (g·L−1) | Volatile Solids (g·L−1) | PO43− Removal (%) | SO42− Removal (%) |
---|---|---|---|---|
modified BBM (pH 7) | 2.80 ± 0.11 b,c | 2.59 ± 0.07 b | 69.1 ± 4.2 c,d | 81.2 ± 8.8 a,b |
modified BBM (pH 8) | 2.83 ± 0.14 a,b,c | 2.62 ± 0.11 b | 71.0 ± 4.9 b,c,d | 82.5 ± 10.6 a,b |
10% ADE | 0.44 ± 0.04 e | 0.41 ± 0.04 d | 100 a | 100 a |
10% ADE + P + S | 1.88 ± 0.08 d | 1.76 ± 0.08 c | 49.7 ± 3.6 e | 62.5 ± 9.7 b |
15% ADE + P + S | 2.73 ± 0.12 c | 2.56 ± 0.14 b | 66.3 ± 4.0 d | 75.1 ± 8.5 a,b |
20% ADE + P + S | 3.35 ± 0.21 a,b | 3.12 ± 0.17 a | 75.3 ± 3.8 b,c,d | 92.5 ± 7.1 a,b |
25% ADE + P + S | 3.39 ± 0.23 a | 3.20 ± 0.20 a | 82.2 ± 2.9 b,c | 100 a |
40% ADE + P + S | 2.51 ± 0.09 c | 2.36 ± 0.10 b | 85.1 ± 2.7 b | 77.5 ± 10.6 a,b |
Treatment | Specific Growth Rate (Day−1) | Biomass Productivity (g·L−1·Day−1) | Maximum Pigments (mg·L−1) | Final Pigments (% Dry Weight) |
---|---|---|---|---|
modified BBM (pH 7) | 1.50 ± 0.01 a | 0.60 ± 0.02 a | 80.5 ± 3.0 c | 2.73 ± 0.21 c |
modified BBM (pH 8) | 1.51 ± 0.01 a | 0.60 ± 0.03 a | 86.9 ± 4.0 c | 2.78 ± 0.28 c |
10% ADE | 1.15 ± 0.03 c | 0.09 ± 0.01 d | 4.6 ± 0.7 e | 1.02 ± 0.06 d |
10% ADE + P + S | 1.45 ± 0.01 b | 0.40 ± 0.02 c | 60.9 ± 5.4 d | 2.76 ± 0.11 c |
15% ADE + P + S | 1.06 ± 0.01 d | 0.41 ± 0.02 c | 95.8 ± 6.4 c | 2.97 ± 0.23 c |
20% ADE + P + S | 1.08 ± 0.01 d | 0.50 ± 0.03 b | 124.5 ± 5.6 b | 3.58 ± 0.31 b,c |
25% ADE + P + S | 0.82 ± 0.01 e | 0.39 ± 0.03 c | 160.4 ± 4.2 a | 4.49 ± 0.29 a,b |
40% ADE + P + S | 0.86 ± 0.02 e | 0.33 ± 0.01 c | 124.1 ± 6.2 b | 4.95 ± 0.10 a |
Treatment | Observed OTUs | Chao1 | Shannon | Simpson |
---|---|---|---|---|
15% ADE + P + S | 60 | 62 | 3.99 | 0.86 |
20% ADE + P + S | 59 | 59 | 3.64 | 0.85 |
25% ADE_1 + P + S | 55 | 55 | 3.59 | 0.86 |
25% ADE_2 + P + S | 61 | 61 | 3.74 | 0.85 |
40% ADE + P + S | 61 | 62 | 3.69 | 0.84 |
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Ziganshina, E.E.; Bulynina, S.S.; Ziganshin, A.M. Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent. Plants 2021, 10, 478. https://doi.org/10.3390/plants10030478
Ziganshina EE, Bulynina SS, Ziganshin AM. Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent. Plants. 2021; 10(3):478. https://doi.org/10.3390/plants10030478
Chicago/Turabian StyleZiganshina, Elvira E., Svetlana S. Bulynina, and Ayrat M. Ziganshin. 2021. "Assessment of Chlorella sorokiniana Growth in Anaerobic Digester Effluent" Plants 10, no. 3: 478. https://doi.org/10.3390/plants10030478