A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions
Abstract
:1. Introduction
2. Results
2.1. Isolation of Bacteria
2.2. Inorganic Phosphate Solubilization Tests
2.3. Plant Growth Promotion in Tomato Plants
2.4. Enzymatic Plant Stress-Response
2.5. Gene Expression-Associated with Plant Defense
2.6. Antagonistic Activity of B.c-A against Cmm In Vitro
2.7. Activity of B.c-A against Tomato Bacterial Canker in Greenhouse Tests
3. Discussion
4. Materials and Methods
4.1. Media for Bacterial Cultivation
4.2. Isolation of PGPB
4.3. PCR Amplification of 16S rRNA and Sequence Analysis
4.4. Detection of GA3 by HPLC
4.5. Inorganic Phosphate Solubilization
4.6. Effect on Plant Growth Promotion in Tomato
4.7. Antagonism Test against Clavibacter Michiganensis In Vitro
4.8. Evaluation of Antagonistic Activity of B.c-A against Clavibacter Michiganensis under Greenhouse Conditions
4.9. Stress Response-Antioxidant Enzyme CAT, PAL, SOD Activities Measurements
4.10. Gene Expression-Associated with Plant Defense
4.11. Statistical Analysis
4.12. Ethical Statement
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Solano-Alvarez, N.; Valencia-Hernández, J.A.; Rico-García, E.; Torres-Pacheco, I.; Ocampo-Velázquez, R.V.; Escamilla-Silva, E.M.; Romero-García, A.L.; Alpuche-Solís, Á.G.; Guevara-González, R.G. A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions. Plants 2021, 10, 506. https://doi.org/10.3390/plants10030506
Solano-Alvarez N, Valencia-Hernández JA, Rico-García E, Torres-Pacheco I, Ocampo-Velázquez RV, Escamilla-Silva EM, Romero-García AL, Alpuche-Solís ÁG, Guevara-González RG. A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions. Plants. 2021; 10(3):506. https://doi.org/10.3390/plants10030506
Chicago/Turabian StyleSolano-Alvarez, Nallely, Juan Antonio Valencia-Hernández, Enrique Rico-García, Irineo Torres-Pacheco, Rosalía Virginia Ocampo-Velázquez, Eleazar Máximo Escamilla-Silva, Ana Luz Romero-García, Ángel G. Alpuche-Solís, and Ramón Gerardo Guevara-González. 2021. "A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions" Plants 10, no. 3: 506. https://doi.org/10.3390/plants10030506