Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz
Abstract
:1. Introduction
2. Results
2.1. Seed Development after Self-Pollination and Cross-Pollination of T. koksaghyz
2.2. Evaluation of Pollen Tube Growth by Microscopy
2.3. Transcriptomic Analysis of CP and SP
2.3.1. Filtering and Venn Diagrams
2.3.2. Digital Gene Expression Profile of Selected DEGs
2.3.3. Gene Ontology Analysis of DEGs in the T1 vs. T0 and T2 vs. T1 Comparisons
3. Discussion
4. Materials and Methods
4.1. Plant Material, Cultivation and Pollination
4.2. Confocal Laser Scanning Microscopy (CLSM)
4.3. RNA Extraction, RNA-Seq and Transcriptome Analysis
4.4. Differential Expression Analysis
4.5. GO Enrichment Analysis and Venn Diagrams
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Selected DEGs | Source | BLASTX e-Value 1 | Percent Identity | Description | Abbreviation |
---|---|---|---|---|---|
utg21288.4 | Venn/Visual analysis | 0.0 | 82.67% | leucine-rich repeat extensin-like protein 4 [Lactuca sativa] | LRX4 |
utg8190.13 | Venn/Visual analysis | 9 × 10−150 | 99.55% | tubulin beta chain-like [Lactuca sativa] | TUBB |
utg9900.15 | Visual analysis | 0.0 | 91.77% | probable xyloglucan endotransglucosylase/hydrolase protein 33 [Lactuca sativa] | XTH33 |
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Wollenweber, T.E.; van Deenen, N.; Roelfs, K.-U.; Prüfer, D.; Gronover, C.S. Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz. Plants 2021, 10, 555. https://doi.org/10.3390/plants10030555
Wollenweber TE, van Deenen N, Roelfs K-U, Prüfer D, Gronover CS. Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz. Plants. 2021; 10(3):555. https://doi.org/10.3390/plants10030555
Chicago/Turabian StyleWollenweber, Tassilo Erik, Nicole van Deenen, Kai-Uwe Roelfs, Dirk Prüfer, and Christian Schulze Gronover. 2021. "Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz" Plants 10, no. 3: 555. https://doi.org/10.3390/plants10030555