Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L.
Abstract
:1. Introduction
2. Results
2.1. Low Light’s Effects on the Densities of Peltate Glandular Trichomes and Chemical Properties of Essential Oil Composition of Mint Leaves
2.2. Illumina HiSeq mRNA Sequencing
2.3. Different Gene Expression Analysis
2.4. DEGs Involved in Terpenoids and Menthol Biosynthesis
2.5. Expression of Genes Involved in the Light Signal Transduction Pathway
2.6. Light-Mediated Regulation of Transcription Factors
2.7. Validation of Expression Patterns of Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatment
4.2. Statistics of Peltate Glandular Trichomes Densities and Measurement of Essential Oil Composition in Mint Leaves under Darkness Treatment
4.3. Total RNA Extraction, Library Construction and Transcriptome Sequencing
4.4. De Novo Assembly and Functional Annotation
4.5. Identification and Analysis of Differentially Expressed Genes
4.6. Real-Time Quantitative PCR Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
Abbreviations
PTGs | Peltate glandular trichomes |
GC-MS | Gas chromatography mass spectrometry |
DEGs | Differentially Expressed Genes |
RPKM | Reads per Kb per Million Reads |
qRT-PCR | Quantitative Real-time PCR |
TF | Transcription Factor |
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NO. | Compounds | Percentage of Total Monoterpenes +/−SD | Fold Change Low Light/Control Light | |
---|---|---|---|---|
Control Light | Low Light | |||
1 | (+)α-Pinene | 4.07 ± 0.61 | 3.52 ± 0.59 | 0.86 |
2 | Sabinene | 0.24 ± 0.03 | 0.12 ± 0.03 | 0.50 |
3 | β-Pinene | 1.05 ± 0.15 | 0.93 ± (0.08) | 0.88 |
4 | β-Myrcene | 2.35 ± 0.14 | 1.90 ± (0.35) | 0.81 |
5 | Limonene | 10.49 ± 1.29 | 11.07 ± (1.84) | 1.05 |
6 | 1,8-Cineole | 0.17 ± 0.02 | 0.17 ± 0.01 | 1.00 |
7 | Menthone | 25.18 ± 0.06 | 17.97 ± 0.28 | 0.71 * |
8 | Isomenthone | 7.52 ± 0.49 | 3.12 ± 0.05 | 0.41 * |
9 | Menthol | 32.02 ± 4.69 | 12.85 ± 0.41 | 0.40 * |
10 | Isopulegone | 1.91 ± 0.15 | 0.15 ± 0.02 | 0.08 * |
12 | Pulegone | 0.95 ± 0.22 | 8.70 ± 1.15 | 9.15 ** |
13 | Piperitone | 1.23 ± 0.63 | 28.31 ± 1.14 | 23.02 ** |
14 | Caryophyllene | 0.50 ± 0.06 | 0.16 ± 0.03 | 0.32 * |
15 | Germacrene D | 2.9 ± 0.38 | 0.15 ± 0.02 | 0.16 * |
16 | γ-Cadinene | 0.26 ± 0.02 | 0.26 ± 0.02 | 1.00 |
others | 14.38 ± 2.05 | 18.52 ± 0.27 | 1.29 * | |
Monoterpene hydrocarbons | 17.71 ± 2.31 | 16.66 ± 2.26 | 0.94 | |
Oxygenated monoterpenes | 67.67 ± 5.22 | 64.87 ± 2.24 | 0.95 | |
Sesquiterpenes | 10.07 ± 1.38 | 5.20 ± 0.73 | 0.51 * | |
Total essential compound percentage | 99.87 ± 0.08 | 97.13 ± 0.27 | 0.97 |
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Yu, X.; Qi, X.; Li, S.; Fang, H.; Bai, Y.; Li, L.; Liu, D.; Chen, Z.; Li, W.; Liang, C. Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L. Plants 2021, 10, 930. https://doi.org/10.3390/plants10050930
Yu X, Qi X, Li S, Fang H, Bai Y, Li L, Liu D, Chen Z, Li W, Liang C. Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L. Plants. 2021; 10(5):930. https://doi.org/10.3390/plants10050930
Chicago/Turabian StyleYu, Xu, Xiwu Qi, Shumin Li, Hailing Fang, Yang Bai, Li Li, Dongmei Liu, Zequn Chen, Weilin Li, and Chengyuan Liang. 2021. "Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L." Plants 10, no. 5: 930. https://doi.org/10.3390/plants10050930