PRAT Proteins Operate in Organellar Protein Import and Export in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. HP20 and HP30 Interact during the Import of Transit Sequence-Less Precursors into Chloroplasts
2.2. Isolation and Characterization of HP20 and HP30 Knock-Out Mutants of A. thaliana
2.3. Etiolated Athp30-2 Seedlings Show Reduced Viability during Greening
2.4. HP22 Is Part of a Unique Protein Export Pathway from Chloroplasts that Operates during Leaf Senescence
2.5. Isolation of Proteins Interacting with HP22 in Chloroplasts
2.6. Genetic Interaction of HP22 with HP65b
3. Discussion
3.1. Function of HP20, HP30 and HP30-2 in Chloroplasts
3.2. Role of Mitochondrial HP30-2 in planta
3.3. Role of Chloroplast HP22 during Leaf Senescence
4. Materials and Methods
4.1. Arabidopsis Mutants and RNAi Lines
4.2. Plant Growth Conditions
4.3. Seedling Viability Tests
4.4. Pulse-Labeling of Total Leaf Proteins with 35S-Methionine
4.5. Chloroplast Isolation, Amino Acid Uptake and In Organello-Protein Synthesis
4.6. Isolation of Plastid Envelope Protein Complexes
4.7. Plastid Leakage Assay
4.8. Analysis of Mitochondrial Membrane Proteins
4.9. Other Techniques
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
GFP | green fluorescent protein |
HP | hypothetical protein |
PRAT | preprotein and amino acid transporter |
TIC | translocase of the inner envelope of chloroplasts |
TOC | translocase of the outer envelope of chloroplasts |
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Rossig, C.; Gray, J.; Valdes, O.; Springer, A.; Rustgi, S.; von Wettstein, D.; Reinbothe, C.; Rassow, J.; Reinbothe, S. PRAT Proteins Operate in Organellar Protein Import and Export in Arabidopsis thaliana. Plants 2021, 10, 958. https://doi.org/10.3390/plants10050958
Rossig C, Gray J, Valdes O, Springer A, Rustgi S, von Wettstein D, Reinbothe C, Rassow J, Reinbothe S. PRAT Proteins Operate in Organellar Protein Import and Export in Arabidopsis thaliana. Plants. 2021; 10(5):958. https://doi.org/10.3390/plants10050958
Chicago/Turabian StyleRossig, Claudia, John Gray, Oscar Valdes, Armin Springer, Sachin Rustgi, Diter von Wettstein, Christiane Reinbothe, Joachim Rassow, and Steffen Reinbothe. 2021. "PRAT Proteins Operate in Organellar Protein Import and Export in Arabidopsis thaliana" Plants 10, no. 5: 958. https://doi.org/10.3390/plants10050958