Regulation of Glycosylphosphatidylinositol-Anchored Protein (GPI-AP) Expression by F-Box/LRR-Repeat (FBXL) Protein in Wheat (Triticum aestivum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Gene Cloning and Gene Expression Analysis
2.3. Yeast Two-Hybrid (Y2H) Assay and Library Screening
2.4. Co-Transformation and β-Galactosidase (β-Gal) Activity Assay
2.5. Subcellular Localization Analysis and Bimolecular Fluorescence Complementation (BiFC) Assay
2.6. MG132 Treatment and Western Blot Analysis
3. Results
3.1. TaFBXL Isolation and RNA Accumulation Patterns
3.2. TaFBXL Is a Component of the SCF Complex
3.3. TaFBXL Interacts with TaGPI-AP
3.4. Subcellular Localization and Interaction between TaFBXL and TaGPI-AP
3.5. TaGPI-AP Interacts with TaABP1
3.6. Degradation of TaGPI-AP in 26S Proteasome
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Clone No. | Putative Identification | Organism | e-Value |
---|---|---|---|
25, 28 | WESR2 (wheat early salt-stress responding gene 2) | Triticum aestivum | 0.00 |
34 | Signal recognition particle 9-kDa protein | Aegilops tauschii | 0.00 |
38 | Chlorophyll a-b binding protein of LHCII type 1 | Aegilops tauschii | 0.00 |
42 | 40S ribosomal protein S3a | Aegilops tauschii | 0.00 |
45 | Glycosylphosphatidylinositol (GPI)-anchored proteins, At3g06035-like | Aegilops tauschii | 0.00 |
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Hong, M.J.; Kim, J.-B.; Seo, Y.W.; Kim, D.Y. Regulation of Glycosylphosphatidylinositol-Anchored Protein (GPI-AP) Expression by F-Box/LRR-Repeat (FBXL) Protein in Wheat (Triticum aestivum L.). Plants 2021, 10, 1606. https://doi.org/10.3390/plants10081606
Hong MJ, Kim J-B, Seo YW, Kim DY. Regulation of Glycosylphosphatidylinositol-Anchored Protein (GPI-AP) Expression by F-Box/LRR-Repeat (FBXL) Protein in Wheat (Triticum aestivum L.). Plants. 2021; 10(8):1606. https://doi.org/10.3390/plants10081606
Chicago/Turabian StyleHong, Min Jeong, Jin-Baek Kim, Yong Weon Seo, and Dae Yeon Kim. 2021. "Regulation of Glycosylphosphatidylinositol-Anchored Protein (GPI-AP) Expression by F-Box/LRR-Repeat (FBXL) Protein in Wheat (Triticum aestivum L.)" Plants 10, no. 8: 1606. https://doi.org/10.3390/plants10081606