Overexpression of 7-hydroxymethyl Chlorophyll a Reductase from Cucumber in Tobacco Accelerates Dark-Induced Chlorophyll Degradation
Abstract
:1. Introduction
2. Results
2.1. Identification of Cucumber HCAR
2.2. Analysis of CsHCAR Expression Profiles and Subcellular Localization
2.3. Response of CsHCAR to Multiple Phytohormones and Abiotic Stresses
2.4. Overexpression of CsHCAR Promotes Chl Degradation
2.5. Overexpression of CsHCAR Affects Photosynthesis
2.6. Overexpression of CsHCAR Reduces ROS Production
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Identification and Sequence Analysis of HCAR
4.3. Total RNA Extraction and Gene Expression Analysis
4.4. Subcellular Localization of HCAR
4.5. Plasmid Construction and Screening Transgenic Plants
4.6. Protein Extraction and Immunoblotting Analysis
4.7. Measurement of Chl Content
4.8. Determination of Growth, Chl Fluorescence Parameters and Pn
4.9. Measurement of ROS Content and Histochemical Staining
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Accession Number | Location in Chromosome | CDS (bp) | Amino Acid | MW (KDa) | pI |
---|---|---|---|---|---|---|
HCAR | CsaV3_3G011480 | Chr3 | 1380 | 459 | 51.19 | 7.54 |
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Liu, W.; Chen, G.; Chen, J.; Jahan, M.S.; Guo, S.; Wang, Y.; Sun, J. Overexpression of 7-hydroxymethyl Chlorophyll a Reductase from Cucumber in Tobacco Accelerates Dark-Induced Chlorophyll Degradation. Plants 2021, 10, 1820. https://doi.org/10.3390/plants10091820
Liu W, Chen G, Chen J, Jahan MS, Guo S, Wang Y, Sun J. Overexpression of 7-hydroxymethyl Chlorophyll a Reductase from Cucumber in Tobacco Accelerates Dark-Induced Chlorophyll Degradation. Plants. 2021; 10(9):1820. https://doi.org/10.3390/plants10091820
Chicago/Turabian StyleLiu, Weikang, Guangling Chen, Jiaqi Chen, Mohammad Shah Jahan, Shirong Guo, Yu Wang, and Jin Sun. 2021. "Overexpression of 7-hydroxymethyl Chlorophyll a Reductase from Cucumber in Tobacco Accelerates Dark-Induced Chlorophyll Degradation" Plants 10, no. 9: 1820. https://doi.org/10.3390/plants10091820