The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1
Abstract
:1. Introduction
2. Results
2.1. Taxonomic Composition of Bacterial and Fungal Rhizosphere Communities
2.1.1. Bacterial Relative Abundance
2.1.2. Fungal Relative Abundance
2.2. The Pezizaceae Family Is Highly Enriched in the cerk1 Mutant and All Other Taxonomic Ranks
2.3. Compositional Differences in the Microbial Community between Genotypes
3. Discussion
4. Materials and Methods
4.1. Plant and Soil Material Preparation
4.2. Sampling Procedure
4.3. DNA Extraction
4.4. 16S rDNA and ITS Sequencing
4.5. Sequence Processing for 16S and ITS Amplicons
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Microbial Community | Genotypes | α-Diversity (Faith) | β-Diversity (Weighted Unifrac) |
---|---|---|---|
Bacterial | cerk1 and efr1 | 0.83 | 0.75 |
cerk1 and fls2 | 0.14 | 0.16 | |
cerk1 and WT | 0.28 | 0.32 | |
efr1 and fls2 | 0.36 | 0.07 | |
efr1 and WT | 0.33 | 0.29 | |
fls2 and WT | 0.06 | 0.01 | |
Fungi | cerk1 and efr1 | 0.66 | 0.84 |
cerk1 and fls2 | 1 | 0.76 | |
cerk1 and WT | 0.20 | 0.50 | |
efr1 and fls2 | 0.63 | 0.79 | |
efr1 and WT | 0.20 | 0.64 | |
fls2 and WT | 0.20 | 0.73 |
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Fonseca, J.P.; Lakshmanan, V.; Boschiero, C.; Mysore, K.S. The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1. Plants 2022, 11, 1323. https://doi.org/10.3390/plants11101323
Fonseca JP, Lakshmanan V, Boschiero C, Mysore KS. The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1. Plants. 2022; 11(10):1323. https://doi.org/10.3390/plants11101323
Chicago/Turabian StyleFonseca, Jose P., Venkatachalam Lakshmanan, Clarissa Boschiero, and Kirankumar S. Mysore. 2022. "The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1" Plants 11, no. 10: 1323. https://doi.org/10.3390/plants11101323