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Volume 11
Issue 10
10.3390/plants11101372
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Article

An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage

by
Wendi Chen
,
Bin Zhang
,
Wenjing Ren
,
Li Chen
,
Zhiyuan Fang
,
Limei Yang
,
Mu Zhuang
,
Honghao Lv
,
Yong Wang
and
Yangyong Zhang
*
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Plants 2022, 11(10), 1372; https://doi.org/10.3390/plants11101372
Submission received: 7 April 2022 / Revised: 11 May 2022 / Accepted: 17 May 2022 / Published: 21 May 2022
(This article belongs to the Special Issue Molecular Breeding for Environmental Stress Resistance in Vegetables)
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Abstract

Cabbage (Brassica oleracea L. var. capitata) self-incompatibility is important for heterosis. However, the seed production of elite hybrid cannot be facilitated by honey bees due to the cross-incompatibility of the two parents. In this study, the self-compatibility of 58 winter cabbage inbred lines was identified by open-flower self-pollination (OS) and molecular techniques. Based on the NCBI database, a new class I S-haplotype-specific marker, PKC6F/PKC6R, was developed. Verification analyses revealed 9 different S-haplotypes in the 58 cabbage inbred lines; of these lines, 46 and 12 belonged to class I (S6, S7, S12, S14, S33, S45, S51, S68) and class II (S15) S-haplotypes, respectively. The coincidence rate between the self-compatibility index and S-haplotype was 91%. This study developed a Tri-Primer-PCR amplification method to rapidly select plants with specific S-haplotypes in biased segregated S-locus populations. Furthermore, it established an S-haplotype identification system based on these nine S-haplotypes. To overcome parental cross-incompatibility (18-503 and 18-512), an inbred line (18-2169) with the S15 haplotype was selected from the sister lines of self-incompatible 18-512 (S68, class I S-haplotype). The inbred line (18-2169) showed self-compatibility and cross-compatibility with 18-503. This study provides guidance for self-compatibility breeding in cabbage and predicts parental cross-incompatibility in elite combinations.
Keywords: cabbage; self-incompatibility; S-haplotype; primer set; cross-incompatibility cabbage; self-incompatibility; S-haplotype; primer set; cross-incompatibility

Share and Cite

MDPI and ACS Style

Chen, W.; Zhang, B.; Ren, W.; Chen, L.; Fang, Z.; Yang, L.; Zhuang, M.; Lv, H.; Wang, Y.; Zhang, Y. An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage. Plants 2022, 11, 1372. https://doi.org/10.3390/plants11101372

AMA Style

Chen W, Zhang B, Ren W, Chen L, Fang Z, Yang L, Zhuang M, Lv H, Wang Y, Zhang Y. An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage. Plants. 2022; 11(10):1372. https://doi.org/10.3390/plants11101372

Chicago/Turabian Style

Chen, Wendi, Bin Zhang, Wenjing Ren, Li Chen, Zhiyuan Fang, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, and Yangyong Zhang. 2022. "An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage" Plants 11, no. 10: 1372. https://doi.org/10.3390/plants11101372

APA Style

Chen, W., Zhang, B., Ren, W., Chen, L., Fang, Z., Yang, L., Zhuang, M., Lv, H., Wang, Y., & Zhang, Y. (2022). An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage. Plants, 11(10), 1372. https://doi.org/10.3390/plants11101372

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