Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes
Abstract
:1. Introduction
2. Results
2.1. Morphological Deformation of Rosa multiflora ‘Libellula’ Leaves Treated with 60Co γ-rays
2.2. Irradiation of Plants with 60Co γ-rays Increased the Accumulation of Active Oxygen and Decreased the Chlorophyll Content
2.3. RNA-Seq Analysis of Differentially Expressed Genes in Irradiated Rose Leaves
2.4. Antioxidant Functions in Irradiated Plants Were Weakened, and the Genes Related to Chlorophyll Synthesis and Metabolism Were Differentially Expressed
2.5. Differential Expression of Genes Related to Leaf Development and Morphogenesis in Irradiated Plants
2.6. Identification and Functional Analysis of Candidate Genes for Leaf Development in Rosa multiflora ‘Libellula’
3. Discussion
4. Materials and Methods
4.1. Plant Material Culture Conditions and Irradiation Treatment Methods
4.2. Determination of Physiological Indicators
4.3. RNA Extraction and Quantitative Real-Time PCR Detection
4.4. Functional Verification of Virus-Induced Gene Silencing (VIGS)
4.5. RNA-Seq Data Processing, Reassembly, and Annotation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group Number | Variation Rate | Incised Leaves | Reduced Number of Compound Leaves | Rolling Leaf | Big Leaf | Biacuminate Leaf | Asymmetric Leaf |
---|---|---|---|---|---|---|---|
control | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
1-1 | 84.89 ± 5.18 de | 17.55 ± 0.48 b | 18.57 ± 0.94 d | 22.92 ± 2.78 c | 9.96 ± 0.46 b | 5.89 ± 0.59 b | 10.00 ± 1.11 cd |
1-2 | 76.36 ± 1.18 e | 26.45 ± 0.47 a | 13.62 ± 0.98 e | 12.80 ± 2.08 d | 8.94 ± 1.11 bc | 3.89 ± 0.48 c | 10.66 ± 0.53 c |
1-3 | 94.26 ± 4.43 d | 3.02 ± 0.72 d | 20.71 ± 0.86 c | 48.70 ± 2.96 b | 14.75 ± 1.52 ab | 1.13 ± 0.21 de | 5.95 ± 0.40 d |
2-1 | 118.08 ± 4.46 b | 15.32 ± 0.57 cd | 32.49 ± 1.27 ab | 48.35 ± 4.06 bc | 5.81 ± 2.50 c | 3.79 ± 1.01 cd | 12.32 ± 0.77 b |
2-2 | 106.36 ± 1.63 c | 16.24 ± 0.39 c | 33.53 ± 1.48 a | 11.21 ± 2.55 de | 16.41 ± 1.58 a | 8.14 ± 1.01 a | 20.83 ± 1.43 a |
2-3 | 141.67 ± 2.71 a | 17.11 ± 1.16 bc | 27.28 ± 1.98 b | 81.06 ± 2.09 a | 4.53 ± 0.44 cd | 1.87 ± 0.50 d | 9.83 ± 1.30 cd |
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Xia, M.; Xu, Q.; Liu, Y.; Ming, F. Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes. Plants 2022, 11, 1438. https://doi.org/10.3390/plants11111438
Xia M, Xu Q, Liu Y, Ming F. Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes. Plants. 2022; 11(11):1438. https://doi.org/10.3390/plants11111438
Chicago/Turabian StyleXia, Meng, Qingyu Xu, Ying Liu, and Feng Ming. 2022. "Mutagenic Effect of 60Co γ-Irradiation on Rosa multiflora ‘Libellula’ and the Mechanism Underlying the Associated Leaf Changes" Plants 11, no. 11: 1438. https://doi.org/10.3390/plants11111438