Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Experiments
2.2. Glasshouse Experiment
Seedling Evaluation at 200 mM NaCl
2.3. Field Experiment
2.3.1. Plant Growth and Field Conditions
2.3.2. Field Evaluation for Morphological Traits
2.3.3. Selection Criteria of Tolerant and Susceptible Lines
2.4. Molecular Characterization
2.4.1. RNA Sampling, Extraction, cDNA Synthesis
2.4.2. Selection of Differentially Expressed Genes (DEGs) and Expression Analysis
2.5. Statistical Analysis
3. Results
3.1. Glasshouse Experiment
3.1.1. Response of Seedlings Growth under Glasshouse Conditions
3.1.2. Mean Variability of Seedlings Parameters
3.1.3. Heritability (H2) of Seedlings Parameters
3.1.4. Correlation among Seedlings Parameters
3.1.5. Principal Component Analysis of Seedlings Parameters
3.2. Field Experiment
3.2.1. Effects of Salinity on Yield Components under Field Conditions
3.2.2. Association among Yield Parameters under Field Conditions
3.2.3. Assessment of Rice Genotypes Using Principal Component Analysis (PCA)
3.2.4. Cluster Analysis of Rice Genotypes
3.3. Molecular Characterization
Expression Profiling Analysis in Salt-tolerant and Susceptible Genotypes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SOV | Genotype (G) | Treatment (T) | G × T | Error | Total | Heritability (H2) |
---|---|---|---|---|---|---|
DF | 23 | 1 | 23 | 92 | 143 | |
SL | 15.15 *** | 80.27 *** | 4.72 * | 2.58 | 72.68 | |
RL | 7.77 ns | 196.88 *** | 5.11 ns | 6.64 | 14.42 | |
TPL | 27.22 ** | 528.6 *** | 14.1 ns | 13.07 | 50.09 | |
SFW | 0.0022 *** | 0.2007 *** | 0.0005 ns | 0.008 | 65.60 | |
RFW | 0.0012 ** | 0.2097 *** | 0.0003 ns | 0.006 | 52.69 | |
SDW | 0.0002 *** | 0.0126 *** | 0.0001 *** | 0.000 | 72.39 | |
RDW | 0.7205 ns | 0.0367 ns | 0.7828 ns | 0.7519 | 0.001 | |
SNC | 4.05 *** | 665.64 *** | 6.6 *** | 0.0864 | 37.57 | |
SKC | 3.65 *** | 108.16 *** | 1.44 *** | 0.1137 | 71.01 | |
RNC | 0.211 *** | 15.34 *** | 0.224 *** | 0.0067 | 47.68 | |
RKC | 0.424 *** | 5.46 *** | 0.455 *** | 0.0068 | 47.85 | |
NKR | 0.23 *** | 38.74 *** | 0.29 *** | 0.0087 | 43.69 |
Trait | Salt-Tolerant Genotypes Names and Mean Values | Salt-Susceptible Genotypes Names and Mean Values |
---|---|---|
SL | S13 (29.91), S1 (28.52), and S22 (27.14) | S3 (20.34), S11(22.92), and S8 (22.97) |
RL | S5 (21.16), S3 (20.83), and S20 (20.41) | S8 (14.81), S24 (15.7), and S9 (16.51) |
TPL | S4 (49.35), S5 (48.33), and S3 (47.6) | S24 (36.86), S9 (37.73), and S19 (39.83) |
SFW | S4 (0.159), S5 (0.142), and S3 (0.141) | S24 (0.080), S9 (0.088), and S19 (0.094) |
RFW | S12 (0.124), S24 (0.122), and S8 (0.113) | S17 (0.064), S7 (0.065), and S21 (0.066) |
SDW | S5 (0.048), S2 (0.047), and S17 (0.045) | S9 (0.026), S19 (0.028), and S21 (0.029) |
RDW | S18 (2.23), S10 (0.022), and S24 (0.020) | S23 (0.012), S3 (0.012), and S1 (0.013) |
SNC | S21 (9.13), S5 (8.83), and S18 (8.63) | S15 (2.93), S22 (3.53), and S11 (3.6) |
SKC | S24 (6.06), S15 (5.93), and S11 (5.46) | S20 (2.43), S7 (2.60), and S12 (2.63) |
RNC | S17 (1.70), S22 (1.67), and S14 (1.62) | S24 (0.85), S12 (0.85), and S16 (0.92) |
RKC | S10 (2.98), S1 (1.97), and S6 (1.91) | S17 (0.85), S23 (0.95), and S22 (1.15) |
NKR | S15 (2.34), S10 (2.15), and S2 (2.12) | S3 (0.76), S16 (0.83), and S4 (1.01) |
SOV | Genotype (G) | Treatment (T) | G × T | Error | Total | Heritability |
---|---|---|---|---|---|---|
DF | 23 | 1 | 23 | 92 | 143 | |
PH | 282.9 *** | 31.0 ns | 14.0 ns | 13.63 | 93.21 | |
TN | 28.3 ** | 1614.7 *** | 10.19 ns | 12.10 | 57.23 | |
GY | 391.56 *** | 104959.36 *** | 151.45 ns | 151.40 | 61.32 | |
SY | 861.98 ** | 12886 *** | 439 ns | 339.18 | 54.35 | |
TGW | 39.21 *** | 205.76 *** | 3.73 ns | 2.01 | 90.89 | |
GL | 4.30 *** | 0.166 ns | 0.155 ns | 0.10 | 96.43 | |
HI | 0.0056 *** | 0.8445 *** | 0.0045 *** | 0.001 | 48.10 |
Trait | Salt-Tolerant Genotypes Names and Mean Values | Salt-Susceptible Genotypes Names and Mean Values |
---|---|---|
PH | S3 (117.53), S23 (114), and S24 (113.8) | S18 (88.33), S8 (92.13), and S5 (93.33) |
TN | S9 (22.2), S19 (22), and S24 (21.8) | S4 (14.33), S2 (14.73), and S6 (15) |
GY | S9 (51.46), S8 (48.8), and S22 (42.66) | S1 (23.93), S6 (24.83), and S24 (27.2) |
SY | S1 (125.6), S20 (115.06), and S23 (108.93) | S8 (43.6), S17 (64.26), and S19 (70.66) |
TGW | S1 (30.62), S20 (24.63), and S19 (24.12) | S4 (17.43), S6 (17.63), and S12 (17.99) |
GL | S1 (12.18), S12 (11.14), and S15 (10.05) | S2 (7.81), S10 (8.49), and S23 (8.81) |
HI | S12 (0.41), S14 (0.37), and S9 (0.36) | S2 (0.15), S5 (0.20), and S1 (0.22) |
SSI | S19 (1.16), S18 (0.94), and S23 (0.94) | S16 (0.743), S17 (0.746), and S2 (0.759) |
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Amanat, M.A.; Naeem, M.K.; Algwaiz, H.I.M.; Uzair, M.; Attia, K.A.; AlKathani, M.D.F.; Zaid, I.U.; Zafar, S.A.; Inam, S.; Fiaz, S.; et al. Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress. Plants 2022, 11, 1461. https://doi.org/10.3390/plants11111461
Amanat MA, Naeem MK, Algwaiz HIM, Uzair M, Attia KA, AlKathani MDF, Zaid IU, Zafar SA, Inam S, Fiaz S, et al. Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress. Plants. 2022; 11(11):1461. https://doi.org/10.3390/plants11111461
Chicago/Turabian StyleAmanat, Muhammad Ammar, Muhammad Kashif Naeem, Hussah I. M. Algwaiz, Muhammad Uzair, Kotb A. Attia, Muneera D. F. AlKathani, Imdad Ulah Zaid, Syed Adeel Zafar, Safeena Inam, Sajid Fiaz, and et al. 2022. "Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress" Plants 11, no. 11: 1461. https://doi.org/10.3390/plants11111461