Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri)
Abstract
:1. Introduction
2. Results
2.1. Identification and Sequence Analysis of Pyrus MAPKKs and MAPKKKs
2.2. Phylogenetic Analysis and Multiple Alignment of MAPKK and MAPKKK Genes in Pyrus
2.3. Gene Structure, Conserved Motifs and Chromosomal Distribution Analysis of Pyrus MAPKKs and MAPKKKs
2.4. Expression Profiles of MAPK Cascade Genes in Different Stages of Dormancy in Pear
2.5. MAPK Cascade Networks and Their Co-Expression during Bud Dormancy
2.6. Validation of PbrMAPKKK-PbrMAPKK-PbrMAPK Cascade Gene Function in Pear Dormancy by qRT–PCR Analysis
3. Discussion
4. Materials and Methods
4.1. Identification of the MAPKK and MAPKKK Gene Families in Pyrus
4.2. Multiple Sequence Alignments and Phylogenetic Analysis
4.3. Sequence, Protein Property and Chromosome Location Analyses
4.4. Differential Expression Analysis of MAPK Cascade Genes during Dormancy Using RNA-Seq
4.5. Plant Materials and Treatments
4.6. MAPK Signalling Cascade Co-Expression Analysis during Pear Dormancy
4.7. RNA Isolation and qRT–PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Differentially Expressed Gene Set | Differentially Expressed Gene Number | Up Regulated | Down Regulated |
---|---|---|---|
Day 0 vs. Day 15 | 66 | 32 | 34 |
Day 15 vs. Day 30 | 22 | 9 | 13 |
Day 30 vs. Day 45 | 66 | 47 | 19 |
Process | Temperature | Time | Cycle Number |
---|---|---|---|
Initial Denaturation | 94 °C | 30 s | 1 |
Denaturation | 94 °C | 5 s | 40 |
Annealing | 60 °C | 30 s | |
Extension/Elongation | 72 °C | 10 s |
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Liang, Q.; Lin, X.; Liu, J.; Feng, Y.; Niu, X.; Wang, C.; Song, K.; Yang, C.; Li, L.; Li, Y. Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri). Plants 2022, 11, 1731. https://doi.org/10.3390/plants11131731
Liang Q, Lin X, Liu J, Feng Y, Niu X, Wang C, Song K, Yang C, Li L, Li Y. Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri). Plants. 2022; 11(13):1731. https://doi.org/10.3390/plants11131731
Chicago/Turabian StyleLiang, Qin, Xiaojie Lin, Jinhang Liu, Yu Feng, Xianqian Niu, Chao Wang, Keke Song, Chao Yang, Liang Li, and Yongyu Li. 2022. "Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri)" Plants 11, no. 13: 1731. https://doi.org/10.3390/plants11131731