Gb_ANR-47 Enhances the Resistance of Gossypium barbadense to Fusarium oxysporum f. sp. vasinfectum (FOV) by Regulating the Content of Proanthocyanidins
Abstract
:1. Introduction
2. Results
2.1. Identification of the ANR Gene Family Members in Cotton
2.2. Evolutionary Analysis of the ANR Genes in Cotton
2.3. Evolutionary Tree, Gene Structure, and Motif Analysis of Cotton ANR Genes
2.4. Analysis of Cis-Acting Elements of ANR Genes
2.5. ANR Gene Expression Analysis of G. barbadense
2.6. qRT–PCR
2.7. VIGS
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification and Bioinformatics Analysis of the ANR Genes in Cotton
4.3. Phylogenetic and Collinearity Analysis of ANR Gene Family Members in Cotton
4.4. Chromosomal Location, Gene Structure, and Motif Analysis of the ANR Gene Family
Members in Cotton
4.5. Analysis of Cis-Acting Elements Upstream of the G. barbadense ANR Gene
4.6. RNA Sequencing (RNA-seq) Analysis
4.7. qRT–PCR
4.8. VIGS
4.9. Determination of Anthocyanin Content
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | Open Reading Frame/bp | Protein Length/aa | Relative Molecular Weight(r)/kDa | Theoretical Isoelectric Point (pI) | Subcellular Localization |
---|---|---|---|---|---|---|
GbANR-01 | Gb_A01G0696 | 1062 | 353 | 38.66 | 5.65 | cytoplasm |
GbANR-02 | Gb_A01G2252 | 672 | 223 | 24.64 | 7.54 | nucleus |
GbANR-03 | Gb_A02G1539 | 900 | 299 | 33.45 | 5.31 | chloroplast |
GbANR-04 | Gb_A02G1557 | 975 | 324 | 35.44 | 8.71 | cytoplasm |
GbANR-05 | Gb_A02G1593 | 996 | 331 | 36.10 | 6.36 | cytoplasm |
GbANR-06 | Gb_A05G0439 | 987 | 328 | 35.84 | 5.83 | cytoplasm |
GbANR-07 | Gb_A05G0715 | 984 | 327 | 35.62 | 5.77 | cytoplasm |
GbANR-08 | Gb_A05G1219 | 1017 | 338 | 37.23 | 6.33 | cytoplasm |
GbANR-09 | Gb_A05G1702 | 1011 | 336 | 36.27 | 5.54 | cytoplasm |
GbANR-10 | Gb_A05G1949 | 1068 | 355 | 39.65 | 5.67 | cytoplasm |
GbANR-11 | Gb_A05G2812 | 453 | 150 | 16.27 | 8.84 | extracellular space |
GbANR-12 | Gb_A05G2814 | 960 | 319 | 35.12 | 6.66 | cytoplasm |
GbANR-13 | Gb_A05G3871 | 1104 | 367 | 41.08 | 6.06 | nucleus |
GbANR-14 | Gb_A06G0087 | 1056 | 351 | 39.04 | 5.45 | cytoplasm |
GbANR-15 | Gb_A07G0794 | 1035 | 344 | 38.50 | 6.48 | nucleus |
GbANR-16 | Gb_A07G1334 | 726 | 241 | 25.89 | 8.10 | organelle membrane |
GbANR-17 | Gb_A07G1335 | 990 | 329 | 35.89 | 6.46 | cytoplasm |
GbANR-18 | Gb_A07G1511 | 1020 | 339 | 36.68 | 7.04 | endomembrane system |
GbANR-19 | Gb_A07G1592 | 990 | 329 | 36.12 | 5.55 | cytoplasm |
GbANR-20 | Gb_A07G1593 | 891 | 296 | 32.25 | 5.02 | cytoplasm |
GbANR-21 | Gb_A07G1594 | 1230 | 409 | 46.21 | 9.22 | endomembrane system |
GbANR-22 | Gb_A07G1596 | 984 | 327 | 35.88 | 5.94 | cytoplasm |
GbANR-23 | Gb_A08G1080 | 897 | 298 | 33.21 | 5.02 | cytoplasm |
GbANR-24 | Gb_A08G1297 | 1116 | 371 | 41.31 | 5.68 | nucleus |
GbANR-25 | Gb_A08G1918 | 918 | 305 | 34.32 | 5.34 | nucleus |
GbANR-26 | Gb_A08G2417 | 966 | 321 | 35.37 | 5.62 | endomembrane system |
GbANR-27 | Gb_A09G0335 | 1083 | 360 | 39.91 | 6.04 | cytoplasm |
GbANR-28 | Gb_A09G1409 | 936 | 311 | 34.18 | 8.38 | cytoplasm |
GbANR-29 | Gb_A11G0776 | 1899 | 632 | 70.31 | 5.88 | cytoplasm |
GbANR-30 | Gb_A11G0849 | 744 | 247 | 27.51 | 8.57 | cytoplasm |
GbANR-31 | Gb_A11G1008 | 1017 | 338 | 36.70 | 5.81 | cytoplasm |
GbANR-32 | Gb_A12G0107 | 975 | 324 | 36.05 | 5.89 | cytoplasm |
GbANR-33 | Gb_A12G0108 | 306 | 101 | 10.96 | 8.78 | extracellular space |
GbANR-34 | Gb_A12G0520 | 972 | 323 | 35.73 | 6.66 | cytoplasm |
GbANR-35 | Gb_A12G2641 | 1041 | 346 | 37.76 | 6.51 | plasma membrane |
GbANR-36 | Gb_D01G0734 | 960 | 319 | 34.87 | 5.85 | nucleus |
GbANR-37 | Gb_D01G2338 | 972 | 323 | 35.86 | 5.79 | endomembrane system |
GbANR-38 | Gb_D03G0433 | 996 | 331 | 36.05 | 7.12 | cytoplasm |
GbANR-39 | Gb_D03G0545 | 906 | 301 | 33.94 | 6.02 | nucleus |
GbANR-40 | Gb_D03G0546 | 981 | 326 | 35.66 | 5.73 | organelle membrane |
GbANR-41 | Gb_D03G0549 | 975 | 324 | 35.45 | 8.94 | cytoplasm |
GbANR-42 | Gb_D03G0557 | 900 | 299 | 33.42 | 5.40 | chloroplast |
GbANR-43 | Gb_D04G0591 | 1077 | 358 | 39.86 | 7.56 | chloroplast |
GbANR-44 | Gb_D05G0439 | 990 | 329 | 35.96 | 5.39 | cytoplasm |
GbANR-45 | Gb_D05G0704 | 984 | 327 | 35.60 | 5.77 | cytoplasm |
GbANR-46 | Gb_D05G1207 | 1017 | 338 | 37.19 | 6.33 | cytoplasm |
GbANR-47 | Gb_D05G1725 | 1011 | 336 | 36.34 | 5.55 | cytoplasm |
GbANR-48 | Gb_D05G1976 | 1068 | 355 | 39.75 | 5.41 | cytoplasm |
GbANR-49 | Gb_D05G2804 | 897 | 298 | 33.41 | 8.89 | mitochondrion |
GbANR-50 | Gb_D05G2805 | 960 | 319 | 35.06 | 6.61 | cytoplasm |
GbANR-51 | Gb_D06G0087 | 1056 | 351 | 38.94 | 5.34 | cytoplasm |
GbANR-52 | Gb_D07G0801 | 1035 | 344 | 38.36 | 5.98 | nucleus |
GbANR-53 | Gb_D07G1332 | 573 | 190 | 21.55 | 7.68 | cytoplasm |
GbANR-54 | Gb_D07G1333 | 990 | 329 | 35.79 | 7.04 | cytoplasm |
GbANR-55 | Gb_D07G1524 | 1020 | 339 | 36.64 | 6.78 | endomembrane system |
GbANR-56 | Gb_D07G1612 | 1785 | 594 | 64.74 | 4.92 | cytoplasm |
GbANR-57 | Gb_D07G1616 | 720 | 239 | 26.62 | 6.08 | cytoplasm |
GbANR-58 | Gb_D07G1617 | 984 | 327 | 35.72 | 6.46 | cytoplasm |
GbANR-59 | Gb_D08G1045 | 882 | 293 | 33.14 | 5.02 | cytoplasm |
GbANR-60 | Gb_D08G1399 | 1035 | 344 | 38.30 | 5.54 | endomembrane system |
GbANR-61 | Gb_D08G1912 | 918 | 305 | 34.41 | 5.48 | cytoplasm |
GbANR-62 | Gb_D08G2407 | 972 | 323 | 35.58 | 5.94 | cytoplasm |
GbANR-63 | Gb_D09G0306 | 1083 | 360 | 40.00 | 5.97 | cytoplasm |
GbANR-64 | Gb_D11G0798 | 1899 | 632 | 70.34 | 6.56 | cytoplasm |
GbANR-65 | Gb_D11G0879 | 669 | 222 | 24.52 | 6.45 | cytoplasm |
GbANR-66 | Gb_D11G1037 | 927 | 308 | 33.72 | 5.58 | cytoplasm |
GbANR-67 | Gb_D12G0116 | 1008 | 335 | 37.19 | 6.16 | cytoplasm |
GbANR-68 | Gb_D12G0517 | 846 | 281 | 30.91 | 6.93 | cytoplasm |
GbANR-69 | Gb_D12G2647 | 1044 | 347 | 37.92 | 6.75 | endomembrane system |
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Su, X.; Zhao, J.; Gao, W.; Zu, Q.; Chen, Q.; Li, C.; Qu, Y. Gb_ANR-47 Enhances the Resistance of Gossypium barbadense to Fusarium oxysporum f. sp. vasinfectum (FOV) by Regulating the Content of Proanthocyanidins. Plants 2022, 11, 1902. https://doi.org/10.3390/plants11151902
Su X, Zhao J, Gao W, Zu Q, Chen Q, Li C, Qu Y. Gb_ANR-47 Enhances the Resistance of Gossypium barbadense to Fusarium oxysporum f. sp. vasinfectum (FOV) by Regulating the Content of Proanthocyanidins. Plants. 2022; 11(15):1902. https://doi.org/10.3390/plants11151902
Chicago/Turabian StyleSu, Xuening, Jieyin Zhao, Wenju Gao, Qianli Zu, Quanjia Chen, Chunping Li, and Yanying Qu. 2022. "Gb_ANR-47 Enhances the Resistance of Gossypium barbadense to Fusarium oxysporum f. sp. vasinfectum (FOV) by Regulating the Content of Proanthocyanidins" Plants 11, no. 15: 1902. https://doi.org/10.3390/plants11151902