The Divergence of Chromosome Structures and 45S Ribosomal DNA Organization in Cucumis debilis Inferred by Comparative Molecular Cytogenetic Mapping
Abstract
:1. Introduction
2. Results
2.1. Plant Morphology of C. debilis
2.2. Basic Chromosome Number and Number of 45S rDNA Loci in Cucumis Species
2.3. Chromosomal Organization of 45S rDNA in Cucumis Species
2.4. Clustering Analysis and Ideogram of Cucumis Species
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Chromosome Preparations
4.3. Probe Preparation and FISH
4.4. Phylogenetic Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Accession | Species | Origin | Chromosome Number (2n) | Number of 45S rDNA Pairs | 45S rDNA Location | |
---|---|---|---|---|---|---|
Major | Minor | |||||
VN 181-2 | C. debilis L. | Vietnam | 24 | 2 | 1 | Terminal |
LA 09-106 | C. hystrix L. | Laos | 24 | 2 | 1 | Terminal |
RAR 930024 | C. sativus L. | USSR (Uzbekistan, Kazakhstan, and Kyrgyzstan area) | 14 | 3 | 2 | Interstitial |
P90 | C. melo L. | Japan | 24 | 1 | 1 | Terminal & Interstitial |
US 205 | C. melo L. | India | 24 | 2 | 0 | Terminal |
US 143 | C. metuliferus L. | South Africa | 24 | 2 | 0 | Terminal |
Species | ITS Spacer | trnL Intron | trnL-F Spacer | rpl20-rps12 Spacer | trnS-G Spacer | rbcL Gene | matK Gene |
---|---|---|---|---|---|---|---|
C. debilis | HM596905 | HM597013 | HM597012 | HM596962 | HM597068 | KY434400.1 | KY458068.1 |
C. hystrix | HM596908 | HM597017 | HM597016 | HM596965 | HM597076 | DQ785832 | DQ785846 |
C. sativus | HM596928 | HM597036 | HM597036 | HM596984 | HM597104 | DQ5357471.1 | DQ536662.1 |
C. melo | HM596916 | HM597025 | HM597025 | HM596973 | HM597086 | DQ535800 | DQ536659 |
C. metuliferus | EF093517 | DQ785876 | DQ785876 | DQ785862 | HM597088 | DQ785834 | DQ785848 |
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Setiawan, A.B.; Purwantoro, A.; Teo, C.H.; Nhi, P.T.P.; Kato, K.; Kikuchi, S.; Koba, T. The Divergence of Chromosome Structures and 45S Ribosomal DNA Organization in Cucumis debilis Inferred by Comparative Molecular Cytogenetic Mapping. Plants 2022, 11, 1960. https://doi.org/10.3390/plants11151960
Setiawan AB, Purwantoro A, Teo CH, Nhi PTP, Kato K, Kikuchi S, Koba T. The Divergence of Chromosome Structures and 45S Ribosomal DNA Organization in Cucumis debilis Inferred by Comparative Molecular Cytogenetic Mapping. Plants. 2022; 11(15):1960. https://doi.org/10.3390/plants11151960
Chicago/Turabian StyleSetiawan, Agus Budi, Aziz Purwantoro, Chee How Teo, Phan Thi Phuong Nhi, Kenji Kato, Shinji Kikuchi, and Takato Koba. 2022. "The Divergence of Chromosome Structures and 45S Ribosomal DNA Organization in Cucumis debilis Inferred by Comparative Molecular Cytogenetic Mapping" Plants 11, no. 15: 1960. https://doi.org/10.3390/plants11151960