The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis

Yurkov, Andrey P.; Puzanskiy, Roman K.; Kryukov, Alexey A.; Gorbunova, Anastasiia O.; Kudriashova, Tatyana R.; Jacobi, Lidija M.; Kozhemyakov, Andrei P.; Romanyuk, Daria A.; Aronova, Ekaterina B.; Avdeeva, Galina S.; Yemelyanov, Vladislav V.; Shavarda, Alexey L.; Shishova, Maria F.

doi:10.3390/plants11182338

Open AccessArticle

The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis

by

Andrey P. Yurkov

^1,2,*,†

,

Roman K. Puzanskiy

^3,4,†

,

Alexey A. Kryukov

¹,

Anastasiia O. Gorbunova

¹,

Tatyana R. Kudriashova

¹,

Lidija M. Jacobi

¹,

Andrei P. Kozhemyakov

¹,

Daria A. Romanyuk

¹

,

Ekaterina B. Aronova

²,

Galina S. Avdeeva

³,

Vladislav V. Yemelyanov

³

,

Alexey L. Shavarda

^4,5

and

Maria F. Shishova

^3,*

¹

Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia

²

Graduate School of Biotechnology and Food Science, Peter the Great St. Petersburg Polytechnic University, 194021 St. Petersburg, Russia

³

Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia

⁴

Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia

⁵

Center for Molecular and Cell Technologies, St. Petersburg State University, 199034 St. Petersburg, Russia

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Plants 2022, 11(18), 2338; https://doi.org/10.3390/plants11182338

Submission received: 29 July 2022 / Revised: 25 August 2022 / Accepted: 3 September 2022 / Published: 7 September 2022

(This article belongs to the Special Issue Symbiosis of Plants with Mycorrhizal and Endophytic Fungi)

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Abstract

The nature of plant–fungi interaction at early stages of arbuscular mycorrhiza (AM) development is still a puzzling problem. To investigate the processes behind this interaction, we used the Medicago lupulina MlS-1 line that forms high-efficient AM symbiosis with Rhizophagus irregularis. AM fungus actively colonizes the root system of the host plant and contributes to the formation of effective AM as characterized by a high mycorrhizal growth response (MGR) in the host plant. The present study is aimed at distinguishing the alterations in the M. lupulina root metabolic profile as an indicative marker of effective symbiosis. We examined the root metabolome at the 14th and 24th day after sowing and inoculation (DAS) with low substrate phosphorus levels. A GS-MS analysis detected 316 metabolites. Results indicated that profiles of M. lupulina root metabolites differed from those in leaves previously detected. The roots contained fewer sugars and organic acids. Hence, compounds supporting the growth of mycorrhizal fungus (especially amino acids, specific lipids, and carbohydrates) accumulated, and their presence coincided with intensive development of AM structures. Mycorrhization determined the root metabolite profile to a greater extent than host plant development. The obtained data highlight the importance of active plant–fungi metabolic interaction at early stages of host plant development for the determination of symbiotic efficiency.

Keywords: Medicago lupulina; arbuscular mycorrhiza; Rhizophagus irregularis; symbiotic efficiency; plant development; physiological stage; root; metabolic profile

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MDPI and ACS Style

Yurkov, A.P.; Puzanskiy, R.K.; Kryukov, A.A.; Gorbunova, A.O.; Kudriashova, T.R.; Jacobi, L.M.; Kozhemyakov, A.P.; Romanyuk, D.A.; Aronova, E.B.; Avdeeva, G.S.; et al. The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis. Plants 2022, 11, 2338. https://doi.org/10.3390/plants11182338

AMA Style

Yurkov AP, Puzanskiy RK, Kryukov AA, Gorbunova AO, Kudriashova TR, Jacobi LM, Kozhemyakov AP, Romanyuk DA, Aronova EB, Avdeeva GS, et al. The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis. Plants. 2022; 11(18):2338. https://doi.org/10.3390/plants11182338

Chicago/Turabian Style

Yurkov, Andrey P., Roman K. Puzanskiy, Alexey A. Kryukov, Anastasiia O. Gorbunova, Tatyana R. Kudriashova, Lidija M. Jacobi, Andrei P. Kozhemyakov, Daria A. Romanyuk, Ekaterina B. Aronova, Galina S. Avdeeva, and et al. 2022. "The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis" Plants 11, no. 18: 2338. https://doi.org/10.3390/plants11182338

APA Style

Yurkov, A. P., Puzanskiy, R. K., Kryukov, A. A., Gorbunova, A. O., Kudriashova, T. R., Jacobi, L. M., Kozhemyakov, A. P., Romanyuk, D. A., Aronova, E. B., Avdeeva, G. S., Yemelyanov, V. V., Shavarda, A. L., & Shishova, M. F. (2022). The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis. Plants, 11(18), 2338. https://doi.org/10.3390/plants11182338

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The Role of Medicago lupulina Interaction with Rhizophagus irregularis in the Determination of Root Metabolome at Early Stages of AM Symbiosis

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