Compensatory Effect of the ScGrf3-2R Gene in Semi-Dwarf Spring Triticale (x Triticosecale Wittmack)
Abstract
:1. Introduction
2. Results
2.1. Partial Sequencing of ScGrf3-2R
2.2. Development of Allelic-Specific PCR Marker for ScGrf3-2R
2.3. Association between Genotypes and Valuable Agronomic Traits
2.3.1. Individual Effects of Ddw1 and ScGrf3-2R
2.3.2. Interaction of Ddw1 and ScGrf3-2R
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Field Conditions
4.3. Analysis of Valuable Agronomic Traits
4.4. DNA Extraction, PCR and Sequencing
4.5. Molecular Markers
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Variant | PROVEAN Score | Prediction (Cutoff = −2.5) |
---|---|---|
T263S | 0.440 | Neutral |
G319_F320insG | 0.079 | Neutral |
N359S | 0.759 | Neutral |
Month | Amount of Precipitation, mm | Norm, mm | Average Temperature, °C | Norm, °C | ||
---|---|---|---|---|---|---|
2018 | 2019 | 10 Years | 2018 | 2019 | 10 Years | |
May | 44 | 58 | 61 | 16.1 | 16.2 | 13.6 |
June | 54 | 55 | 78 | 17.2 | 19.6 | 17.3 |
July | 85 | 64 | 84 | 20.3 | 16.7 | 19.7 |
August | 20 | 48 | 78 | 19.8 | 16.4 | 17.6 |
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Chernook, A.G.; Bazhenov, M.S.; Kroupin, P.Y.; Ermolaev, A.S.; Kroupina, A.Y.; Vukovic, M.; Avdeev, S.M.; Karlov, G.I.; Divashuk, M.G. Compensatory Effect of the ScGrf3-2R Gene in Semi-Dwarf Spring Triticale (x Triticosecale Wittmack). Plants 2022, 11, 3032. https://doi.org/10.3390/plants11223032
Chernook AG, Bazhenov MS, Kroupin PY, Ermolaev AS, Kroupina AY, Vukovic M, Avdeev SM, Karlov GI, Divashuk MG. Compensatory Effect of the ScGrf3-2R Gene in Semi-Dwarf Spring Triticale (x Triticosecale Wittmack). Plants. 2022; 11(22):3032. https://doi.org/10.3390/plants11223032
Chicago/Turabian StyleChernook, Anastasiya G., Mikhail S. Bazhenov, Pavel Yu. Kroupin, Aleksey S. Ermolaev, Aleksandra Yu. Kroupina, Milena Vukovic, Sergey M. Avdeev, Gennady I. Karlov, and Mikhail G. Divashuk. 2022. "Compensatory Effect of the ScGrf3-2R Gene in Semi-Dwarf Spring Triticale (x Triticosecale Wittmack)" Plants 11, no. 22: 3032. https://doi.org/10.3390/plants11223032