Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism
Abstract
:1. Introduction
2. Results
2.1. Cytoplasmic Type Composition of Late Blight Resistant Populations
2.2. Effects of Cytoplasmic Type on Reproductive Traits
2.3. Effects of Cytoplasmic Type on Pollen Tube Germination and Fruit and Seed Production in Test Crosses
2.4. Correlations among Traits
2.5. Identification of Potential Fertility Restorer Breeding Lines
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Reproductive Trait Evaluation
4.3. Floral Abnormalities Evaluation
4.4. Test Cross Evaluation
4.5. Statistical Analysis and Software
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Populations | ||||
---|---|---|---|---|
Oldest Maternal Ancestor | Ancestor Generations 1 | B3C3 | LBHT | LBHT x LTVR |
Proportion of D-type breeding lines (N = 83) | 96.8% | 39.5% | 16.2% | |
AC 25953 2 | 8 | 2 | -- | -- |
54-Q-2 | 8 | 16 | -- | -- |
INDIA-832 | 6 | 10 | 3 | -- |
GRETA | 6 | -- | 2 | 2 |
66-563-13C | 6 | 2 | -- | -- |
NEVADA | 6 | 9 | 11 | 3 |
CIP676084 | 5 | 13 | 1 | 1 |
INDIA-1058 B 3 | 5 | 4 | -- | -- |
TXY.4 | 2 | 4 | -- | -- |
Proportion of T-type breeding lines (N = 58) | 3.2% | 58.1% | 83.8% | |
AC 25953 2 | 8 | -- | 2 | 2 |
CIP800122 | 7 | -- | 23 | 29 |
CIP750627 | 5 | 2 | -- | -- |
Proportion of W/γ-type breeding lines (N = 1) | 0% | 2.3% | 0% | |
PG-232 | 2 | -- | 1 | -- |
D and T Cytoplasm | D Cytoplasm | T Cytoplasm | ||||
---|---|---|---|---|---|---|
Fruit Set (%) | Seed Set | Fruit Set (%) | Seed Set | Fruit Set (%) | Seed Set | |
Anther-lobe overlap | 0.29 *** | 0.23 ** | 0.10 | 0.08 | 0.18 | 0.04 |
Pollen viability | −0.17 *** | 0.05 | −0.10 | −0.10 | 0.18 | 0.35 *** |
In vivo pollen tube growth | −0.53 *** | −0.46 *** | −0.28 *** | −0.16 | −0.48 *** | −0.41 *** |
Breeding Lines | |||||||||
---|---|---|---|---|---|---|---|---|---|
D-Type | T-Type | Total MF | Total MS | Candidate | |||||
Parent Type | CIP Number | CyT | MF | MS | MF | MS | |||
Male | CIP392633.64 | D | 3 | 0 | 7 | 0 | 10 | 0 | Rf+ |
CIP396272.43 | D | 0 | 0 | 6 | 0 | 6 | 0 | Rf+ | |
CIP304372.7 | T | 0 | 1 | 4 | 3 | 4 | 4 | Rf+ | |
CIP392639.2 | D | 2 | 2 | 0 | 0 | 2 | 2 | Rf+ | |
CIP395017.229 | D | 2 | 2 | 0 | 0 | 2 | 2 | Rf+ | |
CIP396012.288 | D | 5 | 3 | 0 | 0 | 5 | 3 | Rf+ | |
CIP396038.107 | D | 0 | 6 | 0 | 0 | 0 | 6 | Rf− | |
CIP396041.102 | T | 0 | 4 | 0 | 0 | 0 | 4 | Rf− | |
Female | CIP395117.3 | D | -- | -- | -- | -- | 0 | 2 | Rf− |
CIP396034.103 | D | -- | -- | -- | -- | 0 | 3 | Rf− | |
CIP396046.105 | D | -- | -- | -- | -- | 0 | 3 | Rf− |
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Santayana, M.; Aponte, M.; Kante, M.; Eyzaguirre, R.; Gastelo, M.; Lindqvist-Kreuze, H. Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism. Plants 2022, 11, 3093. https://doi.org/10.3390/plants11223093
Santayana M, Aponte M, Kante M, Eyzaguirre R, Gastelo M, Lindqvist-Kreuze H. Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism. Plants. 2022; 11(22):3093. https://doi.org/10.3390/plants11223093
Chicago/Turabian StyleSantayana, Monica, Mariela Aponte, Moctar Kante, Raúl Eyzaguirre, Manuel Gastelo, and Hannele Lindqvist-Kreuze. 2022. "Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism" Plants 11, no. 22: 3093. https://doi.org/10.3390/plants11223093