Silencing of MsD14 Resulted in Enhanced Forage Biomass through Increasing Shoot Branching in Alfalfa (Medicago sativa L.)
Abstract
:1. Introduction
2. Results
2.1. Identification of the Strigolactone Receptor Gene (D14) in Alfalfa
2.2. Temporal and Spatial Expression Patterns of MsD14
2.3. Subcellular Localization of MsD14
2.4. Silencing of MsD14 Increased the Branching Number in Alfalfa
2.5. Interaction of MsD14 with MAX2
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Nucleic Acid Extraction and PCR
4.3. MsD14 Cloning and Informatics Analysis
4.4. GUS Histochemical Assay
4.5. Subcellular Localization Analysis
4.6. Alfalfa Expression Vector Construction and Genetic Transformation
4.7. Yeast Two-Hybrid Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, L.; Zhang, Y.; Wen, H.; Liu, W.; Zhou, Y.; Wang, X. Silencing of MsD14 Resulted in Enhanced Forage Biomass through Increasing Shoot Branching in Alfalfa (Medicago sativa L.). Plants 2022, 11, 939. https://doi.org/10.3390/plants11070939
Ma L, Zhang Y, Wen H, Liu W, Zhou Y, Wang X. Silencing of MsD14 Resulted in Enhanced Forage Biomass through Increasing Shoot Branching in Alfalfa (Medicago sativa L.). Plants. 2022; 11(7):939. https://doi.org/10.3390/plants11070939
Chicago/Turabian StyleMa, Lin, Yongchao Zhang, Hongyu Wen, Wenhui Liu, Yu Zhou, and Xuemin Wang. 2022. "Silencing of MsD14 Resulted in Enhanced Forage Biomass through Increasing Shoot Branching in Alfalfa (Medicago sativa L.)" Plants 11, no. 7: 939. https://doi.org/10.3390/plants11070939