Next Article in Journal
Machine Learning for Plant Stress Modeling: A Perspective towards Hormesis Management
Next Article in Special Issue
Seasonal Variation in Selected Biochemical Traits in the Leaves of Co-Occurring Invasive and Native Plant Species under Mediterranean Conditions
Previous Article in Journal
Intraspecific Diversity in Aquatic Ecosystems: Comparison between Spirodela polyrhiza and Lemna minor in Natural Populations of Duckweed
Previous Article in Special Issue
Allelopathy of Knotweeds as Invasive Plants
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 11
Issue 7
10.3390/plants11070969
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Factors Affecting Seed Germination of the Invasive Species Symphyotrichum lanceolatum and Their Implication for Invasion Success

by
Marija Nešić
1,*,
Dragica Obratov-Petković
1,
Dragana Skočajić
1,
Ivana Bjedov
1 and
Nevena Čule
2
1
Department of Landscape Architecture and Horticulture, Faculty of Forestry, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia
2
Department of Environmental Protection and Improvement, Institute of Forestry, Kneza Višeslava 3, 11030 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Plants 2022, 11(7), 969; https://doi.org/10.3390/plants11070969
Submission received: 23 February 2022 / Revised: 20 March 2022 / Accepted: 21 March 2022 / Published: 1 April 2022
(This article belongs to the Special Issue Alien Flora—Adaptation to Novel Ecosystems and Traits for Success)
Browse Figures
Versions Notes

Abstract

:
Invasive species Symphyotrichum lanceolatum (Willd.) G. L. Nesom is spreading uncontrollably along wet habitats as well as in disturbed ecosystems. All those habitats function as corridors that facilitate seed dispersal. One way to prevent the spread of invasive species is to know their reproductive ecology. The present study evaluates the potential for generative reproduction of S. lanceolatum and determines how different temperatures, amounts of nutrients, and light regimes, affect seed germination. Seeds collected from 13 natural populations were germinated at four fluctuating temperature regimes (15/6, 20/10, 30/15, and 35/20 °C). To test the influence of nitrate on seed germination, two KNO3 concentrations were used (5 mM and 50 mM solution). For each treatment, three replicates of 30 seeds were placed in complete darkness or a 14 h photoperiod. The results showed that the germination increased with increasing temperature. The optimal temperature regimes were 30/15 °C and 35/20 °C with approximately 88% germination. The overall effect of KNO3 on germination was positive. The concentration of 50 mM KNO3 had a less stimulating effect compared to 5 mM KNO3. Seeds showed sensitivity to lack of light during germination but were able to germinate in a significant percentage in such conditions. Considering that S. lanceolatum often occurs in disturbed sites, these results suggest that seed reaction to alternating temperature, nutrients concentration, and light can be determining factors that affect seed germination of this species and, thus, its spread.

1. Introduction

Invasive species are one of the biggest threats to world biodiversity, contributing to landscape homogenization by forming large but species-poor stands [1]. By the most frequently used definition, invasive plants have the potential to spread over large areas by producing reproductive offspring, often in large numbers and at considerable distances from the parent plant [2].
When an introduced species enters a new habitat outside its previous geographical range, it faces new climatic and habitat conditions. This poses a challenge for introduced species to establish a population that will further spread to new habitats [3]. Moravcova et al. [4] found that reproductive traits, especially those related to seed production and dispersal, have the greatest impact on the assessment of introduced plants invasiveness.
The size and number of seeds produced by invasive plants affect the species’ ability to colonize new habitats and to form a stable population [5]. The literature on invasive species traits has highlighted that many invasive species produce large quantities of small seeds [6,7,8,9]. Symphyotrichum lanceolatum (Willd.) G. L. Nesom (Compositae) is one of the plant species that produce very large numbers of small seeds. Small seeds usually remain viable longer in the soil and form more permanent seed banks [4,10]. Equally important is that plants with the ability to quickly colonize disturbed habitats have small seeds that are dispersed over long distances. Hence, species with such seeds are more likely to become invasive [4,8,11].
Propagule pressure is a term that describes the number or rate at which propagules enter a particular area [12]. However, the quality of propagules should be considered when assessing the propagule pressure or the probability of species invasion [13]. Propagule pressure is a factor that plays a crucial role in the colonization of new habitats [14,15].
The spread of introduced species continues due to adaptions for seed dispersal [16]. Generative reproduction allows propagule dispersal over long distances. In contrast, vegetative propagules rarely possess mechanisms that allow them to be dispersed over long distances [17,18]. Nevertheless, it is also important to note that there are many cases of introduction of clones or vegetative propagules (bulbs, bulbils) that are at the base of many cases of invasion [19,20,21].
The seed is sensitive to changes in environmental conditions such as temperature, light, moisture, and nitrate content [22]. Nitrate can regulate germination both alone or in combination with other abiotic factors, such as alternating temperature or light [22,23]. Increased nitrogen availability in soil could help break seed dormancy and stimulate germination [24]. Furthermore, an increase in the amount of nitrogen in the soil due to the deposition of atmospheric nitrogen can increase the dominance of invasive plant species [25]. The seed reaction to light is also the mechanism by which the seed responds to the sudden disappearance of vegetation after detecting a high concentration of nutrients, and thus the lack of competition [26]. The positive reaction of the seed to alternating temperatures and the increased nitrate content is also the mechanism by which the seed reacts to the vegetation gaps. Plants that occupy clearings and have strong growth have an advantage over plants that will appear in the later stages of succession when the competition for resources is higher [27].
The joint effects of several environmental factors are implied in natural habitats [26]. Even though such interactions complicate the interpretation of the experimental results, they provide an assumption of species functioning under natural conditions. Vincent and Roberts [28] found that the combination of cold stratification, nitrate, alternating temperature, and different light regimes has several positive effects on the seeds of many weed species [29].
S. lanceolatum is a tall, herbaceous perennial occupying early successional habitats. The species is native to North America and Canada, and invasive in many European countries [30]. S. lanceolatum blooms and bears fruits in late summer to late autumn and aboveground parts die back subsequently [31,32]. Achenes mature 3 to 4 weeks after pollination, and new seedlings occur in spring [31]. This species inhabits moist, nitrogen-rich soil along riverbeds and lakes, ponds, roads, as well as wet ecosystems in which disturbances are expressed [33,34,35,36]. All these habitats act as corridors through which plants spread.
The balance between generative and vegetative reproduction, seed size, dormancy, seed dispersal time and mechanisms, and life span is the main reproductive characteristic of wetland plants [37]. Accordingly, we anticipated that traits that favor S. lanceolatum over native species and allow this species to quickly colonize new habitats are related to its reproductive potential. Therefore, the present study evaluates the generative reproduction of S. lanceolatum and determines how different light regimes, temperatures, and amounts of nutrients affect seed germination. Considering that S. lanceolatum occupies habitats in which disturbances are expressed, it is expected that alternating temperatures and increased nutrients will have a positive effect on seed germination.

2. Results

2.1. Generalized Effect of Alternating Temperatures, Nitrates, and Light on Seed Germination

Three-way ANOVA was used to test the effects of temperature, KNO3 concentration, and light condition on germinative capacity (GC). Looking at Table 1, it is apparent that GC was significantly affected by temperature, light, and KNO3 concentration, as well as by interaction between temperature and light, temperature and KNO3 concentrations, and light and KNO3 concentrations.
Figure 1 displays cumulative germination data for all combinations of incubation temperature and nitrate concentration under light conditions. As can be seen, the germination dynamic was similar at 15/6 °C and 20/10 °C on the fourth day and germination was neglectable. However, final germination was higher at 20/10 °C (49.49–65.21%) compared to 15/6 °C (28.46–47.35%). At elevated temperatures (30/15 °C and 35/20 °C), germination was higher compared to other temperature regimes, with germination on the fourth day ranging between 28.7 and 59.4%. After that, a similar germination dynamic can be seen since the percentage of final germinated seeds was around 90% in those elevated temperature regimes.

2.2. Effect of Alternating Temperatures on Seed Germination

Table 2 reveals that there was an increase in GC with increasing temperature, at both light regimes in treatments with KNO3 and control. In all treatments and both light conditions, GC was the lowest at 15/6 °C. Seeds exposed to light germinated in a higher percentage. Further, GC was higher at higher temperatures (30/15 °C and 35/20 °C) in both light regimes. The exception was treatment with 5 mM KNO3 in dark conditions, where GC was significantly lower at 35/20 °C compared to 30/15 °C treatment (Table 2).

2.3. Effect of Nitrate on Seed Germination

Figure 2 shows that a significant increase in GC was caused by both KNO3 treatments compared to control in light at all temperature regimes except for 35/20 °C, where the increase was not significant. The 5 mM KNO3 treatment also had a positive effect on GC increase in the dark, except at a temperature of 35/20 °C, where GC was lower, but without statistical significance (p > 0.05). Treatment with 50 mM KNO3 increased the GC compared to control (p < 0.05) at all temperature regimes, except at 35/20 °C in light conditions (Figure 2). In dark conditions, GC increased only at 15/6 °C. The 50 mM KNO3 treatment did not have a statistically significant effect on GC compared to control at 20/10 °C and 30/15 °C in dark. Furthermore, GC was significantly lower at 35/20 °C compared to control.

2.4. Effect of Light on Seed Germination

As expected, seed germination was inhibited in the dark treatments (Table 3). Results showed that GC in the dark was lower than GC in the light (Figure 3). The lack of light significantly reduced the GC by 50.75% in the treatment with 0 M KNO3 (control) at 15/6 °C (Table 3, Figure 3a). A statistically significant decrease was also recorded at temperatures 30/15 °C and 35/20 °C in all KNO3 treatments (Figure 3a–c). The smallest germination inhibition by dark was in the treatment with 5 mM KNO3 at 15/6 °C (Table 3). This result is in line with the results of the ANOVA test (Figure 3b) which showed no significant difference between GC under light and dark conditions. In contrast, in the treatment with 50 mM KNO3, a statistically significant inhibition of germination was recorded at 15/6 °C (Figure 3c), pointing to the highest germination inhibition (37.63%) by this treatment (Table 3).

3. Discussion

Jones (1978) showed that S. lanceolatum germination percentage ranges between 60–80% in seeds that have not been stratified, while after cold stratification this percentage was even higher. However, Schmid and Bazzaz [38] determined that the S. lanceolatum germination percentage for seed germinated at a 24 °C day and an 18 °C night temperature cycle ranged between 39.5–43.7%. Our previous study showed that the S. lanceolatum germination percentage was below 46% for seeds that germinated at 20 °C [32]. In contrast, the current study showed much higher germination in elevated, alternating temperatures. Germination was also enhanced in treatments with potassium nitrate. Although the results showed a higher percentage of germinated achenes in relation to our initial research, these results differ from the Jedlička and Prach [30] study which suggested very high germination, even 100%, for seeds that were germinated immediately after collection, after storage at room temperature for 5 months or after storage at 5 °C for 5 months, at 25/15 °C day–night temperature regime. Our study has been unable to demonstrate such a high percentage of germination for seeds that have been stored at a temperature between 2.3 and 6.9 °C. Interspecific hybridization between species of Symphyotrichum is very common [31]. It may be the case, therefore, that these variations could have contributed to different germination percentages. In the case of hybrids, germination can be as low as 10% [39].
In seed ecology, temperature has a dual role. It affects dormancy as well as seed germination [40,41]. S. lanceolatum seeds ripen in late November or early December when their dispersal begins and lasts throughout the winter. Seeds that fall on the soil surface during autumn and winter do not germinate, because the minimum temperature required for germination is higher than the air and soil temperature in that period [42]. Further, according to the same authors, seed stratification during the winter, at low temperatures, reduces the minimum temperature required for germination. This process allows the seeds of genus Symphyotrichum to germinate in the spring at a temperature that would be low for seed germination during the fall. In non-dormant seeds, temperature affects the percentage of seed germination [43]. Considering that the seeds used in this experiment underwent a process of after-ripening for 4 months, at a temperature between 2.3 and 6.9 °C which overcame seed dormancy, it can be concluded that the temperature had a significant effect on the germination parameters, which is confirmed with ANOVA tests. This claim is also supported by the results of our previous research, with seeds collected from the same populations [32]. In this research, the seeds were stored at 5 °C for an after-ripening period of 4 months and were germinated at 20 °C as advised by the ISTA standards [44] for species of genus Symphyotrichum, while the light regime was 16/8 h. The study showed low seed germination (from 0.58% to 45.98%). In the current research, germinative capacity at 20/10 °C, under light conditions, ranged from 3.33 to 53.33%. However, the reason for higher germination might be the alternating temperature, which in some species has a positive effect on germination parameters [45]. Our results support this claim since GC was significantly higher at elevated temperatures (30/15 °C and 35/20 °C) with germination reaching 88%.
Nitrates affect the loss of dormancy [29,46], and in seeds that are not dormant, they can increase germination [45]. Seed germination under the influence of nitrate is also affected by other environmental conditions, especially by light and alternating temperature [47]. In this study, potassium nitrate showed a stimulating effect on the seeds of S. lanceolatum. The concentration of 50 mM KNO3 had a less pronounced stimulating effect compared to 5 mM KNO3. However, the inhibitory effect of both KNO3 concentrations was notable in the dark at the highest temperature. As already mentioned, GC increased with temperature which suggests that germination was under thermal control but was also enhanced with KNO3 since germination percentage was even higher when seed germinated in KNO3 treatments, in contrast to control. A positive effect of KNO3 was especially noticeable at 15/6 °C which suggest that KNO3 in both applied concentrations can substitute the requirement of seeds for temperature [42]. Small seeds are often photoblastic, or their germination is significantly inhibited by a lack of light [45,48]. Germination of Symphyotrichum pilosum seeds is controlled by phytochrome [49]. Red and white light encourage germination, and darkness and far-red light inhibit it [42,50]. Seeds of S. lanceolatum showed less sensitivity to lack of light during germination. Unexpectedly, the smallest decrease in seed germination from the darkness treatment was observed at 15/6 °C in the treatment with 5 mM KNO3. In some cases, the inhibitory effect of lack of light can be reduced by the influence of nitrate and alternating temperature [26]. The ability of S. lanceolatum seeds to germinate in the dark is a significant ecological characteristic. This means that if the seed happens to be covered with soil or dry plant material before the end of winter, there is a possibility that it will germinate. This is especially important for the S. lanceolatum reproductive ecology because the species inhabits habitats where disturbances in ecosystems are pronounced [51,52].
The sudden disappearance of vegetation in one part of the ecosystem can occur as a consequence of a disturbance caused by natural or anthropogenic factors. Competition for resources is low in those gaps, so new plants can take advantage of available resources. Seed germination is the most important step in the growth cycle of a plant [53]. To use available resources, plants have developed physiological mechanisms by which they recognize environmental conditions that correspond to the conditions that arise after the sudden disappearance of vegetation [54,55]. Further, an alternation of temperature can stimulate the small seeds’ germination because it indicates the sudden disappearance of vegetation or shallowly buried seeds [45]. Pons [29] points out that the reaction of seeds to nitrates is also a mechanism by which seeds can respond to the sudden disappearance of vegetation after detecting a high concentration of nutrients. Then, Fenner and Thompson [45] suggest that the substantial proportion of red light indicates a lack of vegetation and thus a lack of competition. According to the same authors, the ability to react to some environmental conditions allows the seed to have some control over when and where it will germinate. The current study found that temperature significantly increased germination percentage, especially at elevated temperatures (30/15 °C and 35/20 °C), as well as KNO3 in both concentrations (5 mM and 50 mM). Emphasizing that S. lanceolatum occurs in disturbed ecosystems, taken together, the findings of this study suggest that the reaction to changes in temperature, amount of nutrients, and light is one of the decisive factors influencing the germination of seeds of this species. Seeds were able to germinate at different temperature regimes, nutrient concentrations, and different amounts of available light. These results provide further support for the hypothesis that due to seed characteristics and its adaptability, this species can gain an advantage over other indigenous species that coexist in the same habitat.

4. Materials and Methods

4.1. Experimental Setup

At the initial phase of the investigation, 21 localities were investigated in Serbia. Subsequently, 13 localities where S. lanceolatum occurs with high abundance and frequency were chosen for this study. Seeds from standing plants and different parts of inflorescences were collected from those 13 natural populations during the autumn (November 2017) to test the S. lanceolatum seed germination. All selected localities were located along the ecological corridors of the Sava and the Danube. Eight sites close to the Sava and Danube rivers were selected in the wider territory of Belgrade. Additionally, five more sites were investigated near the Danube River 75 km north of Belgrade. The climate of the researched area was moderately continental, altitudes ranged from 71 to 200 m, and soil was mainly a haplic fluvisol (calcaric).
Mature achenes from all populations were stored in Belgrade for 4 months (from December 2017 to March 2018) at a temperature between 2.3 °C and 6.9 °C. Achenes were stored in dry conditions (after ripening) by exposing them during the fall and winter months to outdoor temperatures. Average monthly air temperatures were 4.8 °C in December 2017, and 5.3 °C, 2.3 °C, 6.9 °C in January, February and March 2018, respectively [56,57]. Subsequently, the germination test was performed by the direct method in the plant growth chamber SANYO MLR-351H. Seed germination was examined concerning several environmental factors. In addition, seed viability percentage was determined by the tetrazolium test [44].
Seeds were placed in Petri dishes (9 cm) between two layers of filter paper moistened with 10 mL of distilled water to determine the effect of 4 alternating temperature regimes on germination [44]. Germination tests were carried out at 4 alternating temperature regimes: 15/6, 20/10, 30/15, and 35/20 °C [42]. Two KNO3 concentrations were used: 5 mM and 50 mM solutions [58,59], on 4 temperature regimes to test the influence of nitrate on seed germination. Distilled water was used for control (0 mM KNO3). For each treatment, three replicates of 30 seeds were placed in complete darkness or a 14 h photoperiod, with a photon flux density of 27 μmol m−2 s−1 which was provided by fifteen, 40 w growth chamber fluorescent lamps. Air humidity was 70%. The effects of treatments applied were quantified as germinative capacity (GC), in both light treatments [44]. GC was calculated after 14 days as the number of seeds that had produced seedlings classified as normal [44], as a proportion (%) of the total number of tested seeds.

4.2. Data Analysis

Germination data were transformed to percentages based on the number of germinated seeds, and the viable seeds were calculated as the sum of fresh non-germinated and germinated seeds, also expressed as the mean (%) of the number of seeds in 3 replicates.
Differences in germination means among treatments were analysed by three-way ANOVA. Three-way ANOVA was used to determine the effects of temperature (T), KNO3 concentration (K), and light condition (L) on germinative capacity (GC). If an ANOVA indicated significant differences in the data, Fisher’s LSD test was used to determine the differences among treatments (p < 0.05). All analyses were performed with Statgraphics Centurion XVI (Statpoint Technologies, Inc., Warrenton, VA, USA).
In treatments with the same temperature regime and the same KNO3 concentration, the effect of light was expressed as % of germination inhibition in the dark relative to seed germination in light, based on the formula:
% growth inhibition = 100 (GCd − GCl)/GCd
where GCd and GCl are seed germinative capacity in treatment in darkness and light, respectively.

5. Conclusions

The present study was designed to determine the potential for generative reproduction of S. lanceolatum. The second aim was to determine how different light regimes, temperatures, and amounts of nutrients affect seed germination. This study has identified that elevated alternating temperature and nutrient concentration increased S. lanceolatum germination. GC was significantly higher at elevated temperatures (30/15 °C and 35/20 °C). Furthermore, potassium nitrate had a positive effect on the seed germination, and higher GC was recorded in the treatment with 5 mM KNO3 compared to the 50 mM KNO3 concentration. Furthermore, S. lanceolatum seeds showed sensitivity to lack of light during germination. However, the seeds have also shown the ability to germinate in a significant percentage in such conditions. The findings of this research provide an insight into one of the possible ways that S. lanceolatum spreads to new habitats, and that is via seeds. Generative reproduction can play a significant role in this process since S. lanceolatum produces a high number of viable seeds which can germinate in a relatively high percentage in different environmental conditions. Considering that S. lanceolatum often occurs in disturbed sites, seed reaction to changes in temperature, nutrient concentration, and light is one of the determining factors that affect seed germination of this species and, thus its spread. Further studies regarding the role of generative reproduction would be worthwhile. These studies should explore whether and at what distance from parent plants seedlings of this species occur. In addition, it would be interesting to determine the seedlings’ survival and whether that is enough to establish new populations.

Author Contributions

Conceptualization, M.N., D.O.-P., D.S. and I.B.; methodology, M.N., D.O.-P., D.S. and I.B.; formal analysis, M.N., D.S. and N.Č.; investigation M.N., D.O.-P., D.S., I.B. and N.Č.; writing—original draft preparation, M.N.; writing—review and editing, M.N., D.O.-P., D.S., I.B. and N.Č.; visualization, N.Č.; supervision, D.O.-P. and I.B. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia for the scientific research of the University of Belgrade–Faculty of Forestry in 2022, grant number 451-03-9/2022-14/200169. The APC was funded by University of Belgrade–Faculty of Forestry.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All data are presented in this manuscript.

Acknowledgments

We would like to pay our gratitude and respect to our colleague Nenad Keča for his invaluable support with lab equipment. Nenad Keča passed away in August 2019. He was a dedicated educator at the Faculty of Forestry in Belgrade and a passionate researcher.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Hejda, M.; Sádlo, J.; Kutlvašr, J.; Petřík, P.; Vitkova, M.; Vojík, M.; Pyšek, P.; Pergl, J. Impact of invasive and native dominants on species richness and diversity of plant communities. Preslia 2021, 93, 181–201. [Google Scholar] [CrossRef]
  2. Richardson, D.M.; Pyšek, P.; Rejmánek, M.; Barbour, M.G.; Panetta, F.D.; West, C.J. Naturalization and invasion of alien plants: Concepts and definitions. Divers. Distrib. 2000, 6, 93–107. [Google Scholar] [CrossRef]
  3. Skočajić, D.; Nešić, M. Invasive Species: Routes of Introduction, Establishment, and Expansion. In Life on Land; Leal Filho, W., Azul, A.M., Brandli, L., Salvia, A.L., Wall, T., Eds.; Springer: Cham, Switzerland, 2021; pp. 1–12. [Google Scholar]
  4. Moravcova, L.; Pyšek, P.; Jarošík, V.; Havlíčková, V.; Zákravský, P. Reproductive characteristics of neophytes in the Czech Republic: Traits of invasive and non-invasive species. Preslia 2010, 82, 365–390. [Google Scholar]
  5. Nešić, M.; Bjedov, I. Habitat Degradation: Pressures, Threats, and Conservation. In Life on Land; Leal Filho, W., Azul, A.M., Brandli, L., Salvia, A.L., Wall, T., Eds.; Springer: Cham, Switzerland, 2021; pp. 501–514. [Google Scholar]
  6. Daehler, C.C. Performance comparisons of co-occurring native and alien invasive plants: Implications for conservation and restoration. Annu. Rev. Ecol. Evol. Syst. 2003, 34, 183–211. [Google Scholar] [CrossRef]
  7. Lake, J.C.; Leishman, M.R. Invasion success of exotic plants in natural ecosystems: The role of disturbance, plant attributes and freedom from herbivores. Biol. Conserv. 2004, 117, 215–226. [Google Scholar] [CrossRef]
  8. Hamilton, M.A.; Murray, B.R.; Cadotte, M.W.; Hose, G.C.; Baker, A.C.; Harris, C.J.; Licari, D. Life-history correlates of plant invasiveness at regional and continental scales. Ecol. Lett. 2005, 8, 1066–1074. [Google Scholar] [CrossRef]
  9. Gioria, M.; Pyšek, P.; Osborne, B.A. Timing is everything: Does early and late germination favor invasions by herbaceous alien plants? J. Plant Ecol. 2018, 11, 4–16. [Google Scholar] [CrossRef]
  10. Thompson, K.; Band, S.; Hodgson, J. Seed size and shape predict persistence in soil. Funct. Ecol. 1993, 7, 236–241. [Google Scholar] [CrossRef] [Green Version]
  11. Rejmánek, M.; Richardson, D.M. What attributes make some plant species more invasive? Ecology 1996, 77, 1655–1661. [Google Scholar] [CrossRef]
  12. Duncan, R. Propagule pressure. In Encyclopedia of Biological Invasions; Simberloff, D., Rejmánek, M., Eds.; University of California Press: Berkeley, CA, USA, 2011; pp. 561–563. [Google Scholar]
  13. Davis, M.A. Invasion Biology; Oxford University Press: Oxford, UK, 2009. [Google Scholar]
  14. Rouget, M.; Richardson, D.M. Inferring process from pattern in plant invasions: A semimechanistic model incorporating propagule pressure and environmental factors. Am. Nat. 2003, 162, 713–724. [Google Scholar] [CrossRef]
  15. Mason, R.A.; Cooke, J.; Moles, A.T.; Leishman, M.R. Reproductive output of invasive versus native plants. Glob. Ecol. Biogeogr. 2008, 17, 633–640. [Google Scholar] [CrossRef]
  16. Sakai, A.K.; Allendorf, F.W.; Holt, J.S.; Lodge, D.M.; Molofsky, J.; With, K.A.; Baughman, S.; Cabin, R.J.; Cohen, J.E.; Ellstrand, N.C.; et al. The population biology of invasive species. Annu. Rev. Ecol. Syst. 2001, 32, 305–332. [Google Scholar] [CrossRef] [Green Version]
  17. Starfinger, U.; Stöcklin, J. Seed, pollen, and clonal dispersal and their role in structuring plant populations. In Progress in Botany/Fortschritte der Botanik; Behnke, H.D., Lüttge, U., Esser, K., Kadereit, J.W., Runge, M., Eds.; Springer: Berlin/Heidelberg, Germany, 1996; Volume 57, pp. 336–355. [Google Scholar]
  18. Lui, K.; Thompson, F.L.; Eckert, C.G. Causes and consequences of extreme variation in reproductive strategy and vegetative growth among invasive populations of a clonal aquatic plant, Butomus umbellatus L. (Butomaceae). Biol. Invasions 2005, 7, 427–444. [Google Scholar] [CrossRef]
  19. Montagnani, C.; Gentili, R.; Brundu, G.; Caronni, S.; Citterio, S. Accidental Introduction and Spread of Top Invasive Alien Plants in the European Union through Human-Mediated Agricultural Pathways: What Should We Expect? Agronomy 2022, 12, 423. [Google Scholar] [CrossRef]
  20. Okada, M.; Grewell, B.J.; Jasieniuk, M. Clonal spread of invasive Ludwigia hexapetala and L. grandiflora in freshwater wetlands of California. Aquat. Bot. 2009, 91, 123–129. [Google Scholar] [CrossRef]
  21. Zhang, Y.Y.; Zhang, D.Y.; Barrett, S.C. Genetic uniformity characterizes the invasive spread of water hyacinth (Eichhornia crassipes), a clonal aquatic plant. Mol. Ecol. 2010, 19, 1774–1786. [Google Scholar] [CrossRef]
  22. Duermeyer, L.; Khodapanahi, E.; Yan, D.; Krapp, A.; Rothstein, S.J.; Nambara, E. Regulation of seed dormancy and germination by nitrate. Seed Sci. Res. 2018, 28, 150–157. [Google Scholar] [CrossRef]
  23. Wala, M.; Kołodziejek, J.; Patykowski, J. Nitrogen signals and their ecological significance for seed germination of ten psammophilous plant species from European dry acidic grasslands. PLoS ONE 2021, 16, e0244737. [Google Scholar] [CrossRef]
  24. Henig-Sever, N.; Eshel, A.; Ne’eman, G. Regulation of the germination of Aleppo pine (Pinus halepensis) by nitrate, ammonium, and gibberellin, and its role in post-fire forest regeneration. Physiol. Plant. 2000, 108, 390–397. [Google Scholar] [CrossRef]
  25. Brooks, M.L. Effects of increased soil nitrogen on the dominance of alien annual plants in the Mojave Desert. J. Appl. Ecol. 2003, 40, 344–353. [Google Scholar] [CrossRef]
  26. Pons, T.L. Seed responses to light. In Seeds: The Ecology of Regeneration in Plant Communities; Fenner, M., Ed.; CABI Publishing: New York, NY, USA, 2000; pp. 237–260. [Google Scholar]
  27. Tilman, D. Resource Competition and Community Structure; Princeton University Press: Princeton, NJ, USA, 1982; Volume 17. [Google Scholar]
  28. Vincent, E.; Roberts, E. The interaction of light, nitrate and alternating temperature in promoting the germination of dormant seeds of common weed species. Seed Sci. Technol. 1977, 5, 659–670. [Google Scholar]
  29. Pons, T.L. Breaking of Seed Dormancy by Nitrate as a Gap Detection Mechanism. Ann. Bot. 1989, 63, 139–143. [Google Scholar] [CrossRef]
  30. Jedlička, J.; Prach, K. A comparison of two North-American asters invading in central Europe. Flora Morphol. Distrib. Funct. Ecol. Plants 2006, 201, 652–657. [Google Scholar] [CrossRef]
  31. Chmielewski, J.G.; Semple, J.C. The biology of Canadian weeds. 113. Symphyotrichum lanceolatum (Willd.) Nesom [Aster lanceolatus Willd.] and S. lateriflorum (L.) Löve & Löve [Aster lateriflorus (L.) Britt.]. Can. J. Plant Sci. 2001, 81, 829–849. [Google Scholar] [CrossRef]
  32. Nešić, M.; Obratov-Petković, D.; Skočajić, D.; Bjedov, I. Seed quantity and quality in fruit heads of Aster lanceolatus Willd.: Implications for invasion success. Bull. Fac. For. 2013, 108, 129–144. [Google Scholar] [CrossRef]
  33. Jones, A.G. Observations on reproduction and phenology in some perennial asters. Am. Midl. Nat. 1978, 99, 184–197. [Google Scholar] [CrossRef]
  34. Obratov-Petković, D.; Bjedov, I.; Radulović, S.; Skočajić, D.; Đunisijević-Bojović, D.; Đukić, M. Ecology and distribution of an invasive species Aster lanceolatus Willd. on wet habitats in Belgrade. Bull. Fac. For. 2009, 100, 159–178. [Google Scholar] [CrossRef]
  35. Nešić, M.; Obratov-Petković, D.; Skočajić, D.; Bjedov, I.; Đukić, M.; Đunisijević-Bojović, D. Allelopathic potential of the invasive species Aster lanceolatus Willd. Period. Biol. 2016, 118, 1–7. [Google Scholar] [CrossRef]
  36. Obratov-Petković, D.; Bjedov, I.; Nešić, M.; Simić, S.B.; Đunisijević-Bojović, D.; Skočajić, D. Impact of invasive Aster lanceolatus populations on soil and flora in urban sites. Pol. J. Ecol. 2016, 64, 289–295. [Google Scholar] [CrossRef]
  37. Naiman, R.J.; Décamps, H. The ecology of interfaces: Riparian zones. Annu. Rev. Ecol. Syst. 1997, 28, 621–658. [Google Scholar] [CrossRef] [Green Version]
  38. Schmid, B.; Bazzaz, F. Plasticity in plant size and architecture in rhizome-derived vs. seed-derived Solidago and Aster. Ecology 1990, 71, 523–535. [Google Scholar] [CrossRef]
  39. Fehér, A. Aster species from North America. In The Most Invasive Plants in Hungary; Botta-Dukat, Z., Balogh, L., Eds.; HAS Institute of Ecology and Botany: Vácrátót, Hungary, 2008; pp. 179–187. [Google Scholar]
  40. Huarte, H.R.; Borlandelli, F.; Varisco, D.; Batlla, D. Understanding dormancy breakage and germination ecology of Cynara cardunculus (Asteraceae). Weed Res. 2018, 58, 450–462. [Google Scholar] [CrossRef]
  41. Huarte, H.R.; Puglia, G.; Prjibelski, A.D.; Raccuia, S.A. Seed transcriptome annotation reveals enhanced expression of genes related to ROS homeostasis and ethylene metabolism at alternating temperatures in wild cardoon. Plants 2020, 9, 1225. [Google Scholar] [CrossRef] [PubMed]
  42. Baskin, J.M.; Baskin, C.C. The germination strategy of oldfield aster (Aster pilosus). Am. J. Bot. 1979, 66, 1–5. [Google Scholar] [CrossRef]
  43. Probert, R.J. The role of temperature in the regulation of seed dormancy and germination. In Seeds: The Ecology of Regeneration in Plant Communities; Fenner, M., Ed.; CABI Publishing: New York, NY, USA, 2000; pp. 261–292. [Google Scholar]
  44. International Seed Testing Association. International Rules for Seed Testing, 2003rd ed.; International Seed Testing Association: Zurich, Switzerland, 2003. [Google Scholar]
  45. Fenner, M.; Thompson, K. The Ecology of Seeds; Cambridge University Press: Cambridge, UK, 2005. [Google Scholar]
  46. Yan, A.; Chen, Z. The control of seed dormancy and germination by temperature, light and nitrate. Bot. Rev. 2020, 86, 39–75. [Google Scholar] [CrossRef]
  47. Probert, R.; Gajjar, K.; Haslam, I. The interactive effects of phytochrome, nitrate and thiourea on the germination response to alternating temperatures in seeds of Ranunculus sceleratus L.: A quantal approach. J. Exp. Bot. 1987, 38, 1012–1025. [Google Scholar] [CrossRef]
  48. Kathpalia, R.; Bhatla, S.C. Seed Dormancy and Germination. In Plant Physiology, Development and Metabolism; Bhatla, S.C., Lal, M.A., Eds.; Springer: Singapore, 2018; pp. 885–906. [Google Scholar]
  49. Baskin, J.; Baskin, C. The light requirement for germination of Aster pilosus seeds: Temporal aspects and ecological consequences. J. Ecol. 1985, 73, 765–773. [Google Scholar] [CrossRef]
  50. Peterson, D.; Bazzaz, F. Life cycle characteristics of Aster pilosus in early succesional habitats. Ecology 1978, 59, 1005–1013. [Google Scholar] [CrossRef]
  51. Nesic, M.; Obratov-Petkovic, D.; Bjedov, I.; Cule, N.; Skocajic, D. Competitive interactions between the invasive Symphyotrichum lanceolatum (Willd.) G. L. Nesom and native Achillea millefolium L. Fresenius Environ. Bull. 2021, 30, 12909–12917. [Google Scholar]
  52. Obratov-Petković, D.; Bjedov, I.; Jurišić, B.; Nešić, M.; Stojanović, V. Relationship between invasive plant species and species richness in urban and suburban habitats of Belgrade (Serbia). Adv. GeoEcol. 2014, 43, 348–359. [Google Scholar]
  53. Parihar, P.; Singh, S.; Singh, R.; Singh, V.P.; Prasad, S.M. Effect of salinity stress on plants and its tolerance strategies: A review. Environ. Sci. Pollut. Res. 2015, 22, 4056–4075. [Google Scholar] [CrossRef] [PubMed]
  54. Vazquez-Yanes, C.; Orozco-Segovia, A. Signals for seeds to sense and respond to gaps. In Exploitation of Environmental Heterogeneity by Plants, Ecophysiological Processes Above- and Belowground; Caldwell, M., Pearce, P., Eds.; Academic Press: San Diego, CA, USA, 1994; pp. 209–236. [Google Scholar]
  55. Guggisberg, A.; Liu, X.; Suter, L.; Mansion, G.; Fischer, M.C.; Fior, S.; Roumet, M.; Kretzschmar, R.; Koch, M.A.; Widmer, A. The genomic basis of adaptation to calcareous and siliceous soils in Arabidopsis lyrata. Mol. Ecol. 2018, 27, 5088–5103. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  56. Republic Hydrometeorological Service of Serbia. Annual Bulletin for Serbia 2017; Republic Hydrometeorological Service of Serbia: Belgrade, Serbia, 2017. Available online: https://www.hidmet.gov.rs/data/klimatologija/eng/2017.pdf (accessed on 16 March 2022).
  57. Republic Hydrometeorological Service of Serbia. Annual Bulletin for Serbia the Year of 2018; Republic Hydrometeorological Service of Serbia: Belgrade, Serbia, 2018. Available online: https://www.hidmet.gov.rs/data/klimatologija/eng/2018.pdf (accessed on 16 March 2022).
  58. Pérez-Fernández, M.; Rodríguez-Echeverría, S. Effect of smoke, charred wood, and nitrogenous compounds on seed germination of ten species from woodland in central-western Spain. J. Chem. Ecol. 2003, 29, 237–251. [Google Scholar] [CrossRef] [PubMed]
  59. Pérez-Fernández, M.; Calvo-Magro, E.; Montanero-Fernández, J.; Oyola-Velasco, J. Seed germination in response to chemicals: Effect of nitrogen and pH in the media. J. Environ. Biol. 2006, 27, 13–20. [Google Scholar] [PubMed]
Plants 11 00969 g001 550
Figure 1. Dynamics of seed germination (%) under light conditions at the different temperature regimes and KNO3 concentration.
Figure 1. Dynamics of seed germination (%) under light conditions at the different temperature regimes and KNO3 concentration.
Plants 11 00969 g001
Plants 11 00969 g002 550
Figure 2. The effect of KNO3 concentration, within the same temperature regime, on germinative capacity (GC) in light and dark treatments. Each value represents the mean ± SE for three repetitions. Mean values within light/dark treatment and same temperature with different letters differ significantly, p < 0.05.
Figure 2. The effect of KNO3 concentration, within the same temperature regime, on germinative capacity (GC) in light and dark treatments. Each value represents the mean ± SE for three repetitions. Mean values within light/dark treatment and same temperature with different letters differ significantly, p < 0.05.
Plants 11 00969 g002
Plants 11 00969 g003a 550Plants 11 00969 g003b 550
Figure 3. The effect of light, within the same temperature regime, on germinative capacity (GC) in treatments with (a) 0 mM KNO3; (b) 5 mM KNO3; (c) 50 mM KNO3. Each value represents the mean ± SE for three replicates. Mean values within the same temperature treatment with different letters differ significantly, p < 0.05.
Figure 3. The effect of light, within the same temperature regime, on germinative capacity (GC) in treatments with (a) 0 mM KNO3; (b) 5 mM KNO3; (c) 50 mM KNO3. Each value represents the mean ± SE for three replicates. Mean values within the same temperature treatment with different letters differ significantly, p < 0.05.
Plants 11 00969 g003aPlants 11 00969 g003b
Table
Table 1. Analysis of variance of germinative capacity (GC) of S. lanceolatum affected by temperature regimes (15/6 °C, 20/10 °C, 30/15 °C, and 35/20 °C), KNO3 concentration (0 mM, 5 mM, and 50 mM) and light regimes (14 h light photoperiod and total darkness).
Table 1. Analysis of variance of germinative capacity (GC) of S. lanceolatum affected by temperature regimes (15/6 °C, 20/10 °C, 30/15 °C, and 35/20 °C), KNO3 concentration (0 mM, 5 mM, and 50 mM) and light regimes (14 h light photoperiod and total darkness).
Source of VariationdfMSFp
Temperature (T)3121,357.95303.84<0.001
Light (L)142,315.05105.94<0.001
KNO3 (K)29324.9423.35<0.001
T × L32132.105.34<0.001
T × K63220.278.06<0.001
L × K22847.567.130.001
T × L × K6503.641.260.272
Error912399.41
Total935
df = degrees of freedom; MS = mean square; F = F-ratio.
Table
Table 2. The effect of alternating temperature and applied KNO3 concentration under the different light conditions on the germinative capacity (GC) of S. lanceolatum.
Table 2. The effect of alternating temperature and applied KNO3 concentration under the different light conditions on the germinative capacity (GC) of S. lanceolatum.
Light TreatmentTemperature (°C)0 mM KNO35 mM KNO350 mM KNO3
Germination Capacity (%)
Light15/628.46 ± 2.72 c47.35 ± 3.21 c41.11 ± 2.78 c
20/1049.49 ± 2.31 b64.19 ± 2.41 b65.21 ± 3.28 b
30/1587.44 ± 1.41 a92.22 ± 0.85 a93.50 ± 0.85 a
35/2087.86 ± 1.97 a90.26 ± 1.26 a89.49 ± 1.17 a
Dark15/614.02 ± 2.39 c45.56 ± 4.76 c25.64 ± 4.12 c
20/1045.98 ± 4.52 b59.83 ± 4.60 b53.33 ± 5.89 b
30/1573.85 ± 3.96 a82.05 ± 2.07 a67.69 ± 4.59 a
35/2076.92 ± 2.58 a69.23 ± 3.65 b61.11 ± 2.33 ab
Each value represents the mean ± SE for three replicates. In the same light treatment, mean values with different letters differ significantly, p < 0.05.
Table
Table 3. Germination inhibition in dark treatments.
Table 3. Germination inhibition in dark treatments.
Temperature (°C)0 mM KNO35 mM KNO350 mM KNO3
Germination Inhibition in Dark Treatments (%)
15/650.753.7937.63
20/107.086.7918.22
30/1515.5411.0327.61
35/2012.4523.3031.71
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Nešić, M.; Obratov-Petković, D.; Skočajić, D.; Bjedov, I.; Čule, N. Factors Affecting Seed Germination of the Invasive Species Symphyotrichum lanceolatum and Their Implication for Invasion Success. Plants 2022, 11, 969. https://doi.org/10.3390/plants11070969

AMA Style

Nešić M, Obratov-Petković D, Skočajić D, Bjedov I, Čule N. Factors Affecting Seed Germination of the Invasive Species Symphyotrichum lanceolatum and Their Implication for Invasion Success. Plants. 2022; 11(7):969. https://doi.org/10.3390/plants11070969

Chicago/Turabian Style

Nešić, Marija, Dragica Obratov-Petković, Dragana Skočajić, Ivana Bjedov, and Nevena Čule. 2022. "Factors Affecting Seed Germination of the Invasive Species Symphyotrichum lanceolatum and Their Implication for Invasion Success" Plants 11, no. 7: 969. https://doi.org/10.3390/plants11070969

APA Style

Nešić, M., Obratov-Petković, D., Skočajić, D., Bjedov, I., & Čule, N. (2022). Factors Affecting Seed Germination of the Invasive Species Symphyotrichum lanceolatum and Their Implication for Invasion Success. Plants, 11(7), 969. https://doi.org/10.3390/plants11070969

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop