Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Heat-Stress Treatment
2.2. RNA Isolation, Microarray Hybridization, Signal Scanning, and Normalization
2.3. Data Filtering, Clustering and Functional Classification
2.4. Quantitative Real-Time PCR
2.5. Cloning of OsNCED1 CDS
2.6. Generation of OsNCED1 Overexpression Lines
2.7. Determination of Expression Pattern and Transcription Levels of OsNCED1 and ABA Signaling Related Genes
2.8. Determination of Pollen Viability, Seed Setting Rate, Cell Membrane Damage, and Oxidative Stress in Transgenic Rice Plants under HS
2.9. Statistical Analysis
3. Results
3.1. Differentially Expressed Gene Analysis under HS
3.2. Comparative Analysis between HTL252 and HSL082
3.3. Quantitative Real-Time PCR (qRT-PCR) Verification
3.4. Expression Pattern of OsNCED1 Gene under HS
3.5. Analysis of Pollen Viability and Seed Setting Rate in OsNCED1 Transgenic Rice Plants
3.6. Analysis of Membrane Permeability and Antioxidant Enzyme Activity of OsNCED1 Transgenic Rice
3.7. Changes in the Transcription of ABA-Signaling-Related Genes in OsNCED1 Transgenic Rice Plants
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, H.; Wang, Y.; Zhang, Y.; Xiao, Y.; Liu, X.; Deng, H.; Lu, X.; Tang, W.; Zhang, G. Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance. Plants 2022, 11, 1062. https://doi.org/10.3390/plants11081062
Zhou H, Wang Y, Zhang Y, Xiao Y, Liu X, Deng H, Lu X, Tang W, Zhang G. Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance. Plants. 2022; 11(8):1062. https://doi.org/10.3390/plants11081062
Chicago/Turabian StyleZhou, Huang, Yingfeng Wang, Yijin Zhang, Yunhua Xiao, Xiong Liu, Huabing Deng, Xuedan Lu, Wenbang Tang, and Guilian Zhang. 2022. "Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance" Plants 11, no. 8: 1062. https://doi.org/10.3390/plants11081062