Nitric Oxide Improves Salt Tolerance of Cyclocarya paliurus by Regulating Endogenous Glutathione Level and Antioxidant Capacity
Abstract
:1. Introduction
2. Results
2.1. Effects of Salt and NO Donors on Total Biomass and Chlorophyll Fluorescence
2.2. Effects of Salt and NO Donors on the Level of Lipid Peroxidation
2.3. Effects of Salt and NO Donors on the Endogenous NO Content and NR Activity
2.4. Effects of Salt and NO Donors on the Endogenous GSH Content and GR Activity
2.5. Effects of Salt and NO Donors on Antioxidant Enzyme Activities
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Treatments
4.2. Chlorophyll Fluorescence Apparatus
4.3. Determination of Total Biomass
4.4. Determination of Reactive Oxygen Species (ROS)
4.5. Assessment of Malondialdehyde Content and the Relative Electrolyte Leakage Rate
4.6. Evaluation of NO and Nitrate Reductase
4.7. Protein Extraction, GSH Content, and Antioxidant Enzymes
4.8. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatments | Chlorophyll Fluorescence Parameters of C. paliurus | ||
---|---|---|---|
Total Biomass | Fv/Fm | NPQ | |
CK | 8.61 ± 0.125 a | 0.77 ± 0.036 a | 0.66 ± 0.032 b |
Salt | 6.33 ± 0.118 e | 0.66 ± 0.016 b | 0.78 ± 0.018 a |
SNAP | 7.40 ± 0.029 c | 0.74 ± 0.058 a | 0.75 ± 0.046 ab |
GSNO | 6.66 ± 0.101 d | 0.74 ± 0.040 a | 0.82 ± 0.073 a |
SNP | 7.67 ± 0.117 b | 0.76 ± 0.008 a | 0.75 ± 0.089 ab |
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Liu, Y.; Yuan, Y.; Jiang, Z.; Jin, S. Nitric Oxide Improves Salt Tolerance of Cyclocarya paliurus by Regulating Endogenous Glutathione Level and Antioxidant Capacity. Plants 2022, 11, 1157. https://doi.org/10.3390/plants11091157
Liu Y, Yuan Y, Jiang Z, Jin S. Nitric Oxide Improves Salt Tolerance of Cyclocarya paliurus by Regulating Endogenous Glutathione Level and Antioxidant Capacity. Plants. 2022; 11(9):1157. https://doi.org/10.3390/plants11091157
Chicago/Turabian StyleLiu, Yang, Yichao Yuan, Zhuoke Jiang, and Songheng Jin. 2022. "Nitric Oxide Improves Salt Tolerance of Cyclocarya paliurus by Regulating Endogenous Glutathione Level and Antioxidant Capacity" Plants 11, no. 9: 1157. https://doi.org/10.3390/plants11091157