Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update
Abstract
:1. Introduction
2. Earlier Identification and Cloning of Fatty Acid Encoding Gene in Perilla
3. Transcriptomics Sheds Lights into Key Master Player Enzymes of Perilla Fatty Acid Biosynthesis
4. Whole-Genome-Driven Fatty Acid Genes Discovery
5. Concluding Remarks and Outlook
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Enzyme ID | Enzyme Name | GeneID | Homologous | Pathways Involved | Field of Study | References | ||
---|---|---|---|---|---|---|---|---|
PF40 * | Dayudeulkkae ** | PC *** | A. Thaliana | |||||
PDH(E1α) | Pyruvate Dehydrogenase E1 Subunit Alpha 1 | Locus_2112 | AT1G01090.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
PDH(E1ß) | Pyruvate Dehydrogenase E1 Subunit beta 1 | Locus_25208 | AT2G34590.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
EMB3003(E2) | Pyruvate dehydrogenase e2 component (dihydrolipoamide acetyltransferase) | Locus_33306 | AT1G34430.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
LTA2 (E2) | Plastid E2 Subunit of Pyruvate Decarboxylase, PLE2 | Locus_5104 | AT3G25860.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
LPD1 (E3) | Lipoamide dehydrogenase | Locus_7407 | AT3G16950.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
α-CTa | Alpha-carboxyltransferase Isoform a | Locus_8492 | AT2G38040.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
α-CTb | Apha-carboxyltransferase Isoform b | Locus_2178 | AT2G38040.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
ß-CT | Beta-carboxyltransferase | Locus_53041 | ATCG00500.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
BC | Biotin carboxylase | Locus_22078 | AT5G35360.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
BCCP1 | Biotin carboxyl carrier protein of acetyl-CoA carboxylase 1 | Locus_29162 | AT5G16390.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
BCCP2 | Biotin carboxyl carrier protein of acetyl-CoA carboxylase 2 | Locus_17340 | AT5G15530.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
MCMT | Malonyl-CoA ACP transacylase | Locus_14579 | AT2G30200.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
KASIII | 3-Ketoacyl-ACP synthase | Locus_10821 | AT1G62640.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
KAR | 3-ketoacyl-ACP reductase | Locus_1445 | AT1G24360.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
HAD | 3-hydroxyacyl-ACP dyhydratase | Locus_19332 | AT5G10160.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
EAR | 2-enoyl-ACP reductase | Locus_25443 | AT2G05990.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
FATA | Fatty acyl-ACP thioesterase A | Locus_29919 | AT3G25110.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
FATB | Fatty acyl-ACP thioesterase B | Locus_6603 | AT1G08510.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
FAB2 | Fatty acid biosynthesis2 | Locus_13564 | AT2G43710.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
DES6 | Stearoyl-acyl carrier protein desaturase | Locus_9486 | AT1G43800.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
KASI | Ketoacyl-ACP Synthase I | Locus_26341 | AT5G46290.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
KASII | Ketoacyl-ACP Synthase II | Locus_1373 | AT1G74960.1 | FA de novo biosynthesis and export from plastid | Transcriptomics | [25] | ||
LACS8 | Long-chain acyl-CoA synthetase 8 | chr07_36292788_36299197 chr19_22302145_22308533 | Locus_3838 | chr06_37084362_37090768 | AT2G04350.1 | FA de novo biosynthesis and export from plastid | Genome Assembly, Transcriptomics | [3,25] |
LACS9 | Long-chain acyl-CoA synthetase 9 | chr03_70622879_70627324 chr09_58852417_58856892 chr01_02424545_02428997 | Locus_23636 | chr01_02424545_02428997 | AT1G77590.1 | FA de novo biosynthesis and export from plastid | Genome Assembly, Transcriptomics | [3,25] |
FAX1 | Fatty acid export 1 | chr05_24282740_24284950 chr01_71691539_71693779 | chr02_42552603_42554830 | FA de novo biosynthesis and export from plastid | [25] | |||
FAX2 | Fatty acid export 2 | chr07_10626150_10628000 | chr06_11381976_11383822 | FA de novo biosynthesis and export from plastid | [25] | |||
FAX3 | Fatty acid export 3 | chr04_00857340_00859552 | chr03_67540865_67543081 | FA de novo biosynthesis and export from plastid | [25] | |||
FAX5 | Fatty acid export 5 | chr04_65527957_65529911 chr07_22534802_22537586 chr06_00746938_00748860 chr19_10735560_10738363 | chr03_02347871_02349825 chr06_23562111_23564893 | FA de novo biosynthesis and export from plastid | [25] | |||
FAD2 | Omega-6 fatty acid desaturase | chr12_56933298_56934446 chr11_05592060_05593208 chr11_05575254_05576393 | Locus_733 | chr08_55538081_55539229 | AT3G12120.1 | Acyl editing of phospatidylcholine | Genome Assembly, Transcriptomics | [3,25] |
chr12_56948107_56949167 | chr08_55558209_55559348 | |||||||
FAD3 | Omega-3 fatty acid desaturase | chr12_04645208_04647776 chr11_54194712_54197265 | Locus_22029 | chr08_04030082_04032640 | AT2G29980.1 | Acyl editing of phospatidylcholine | Genome Assembly, Transcriptomics | [3,25] |
FAD8 | Omega-8 fatty acid desaturase | Locus_5107 | AT5G05580.2 | Acyl editing of phospatidylcholine | Transcriptomics | [25] | ||
GPAT9 | Glycerol-3-phosphate acyltransferase 9 | chr12_33733527_33737891 chr11_26255533_26259881 | Locus_10180 | chr08_33038421_33042132 | AT5G60620.1 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | Genome Assembly, Transcriptomics | [3,25] |
LPAT2 | 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 | chr05_23583386_23588593 chr05_34400913_34404444 chr01_72114246_72119454 | Locus_6587 | chr02_43313059_43318262 chr02_32585727_32589258 | AT3G57650.1 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | Genome Assembly, Transcriptomics | [3,25] |
PAH1 | Phenylalanine hydrolase 1 | chr01_61567423_61570965 chr14_08597119_08602056 chr15_37103964_37108907 chr03_61656532_61661875 chr18_09154357_09159306 chr17_34575710_34580664 chr09_50343045_50349360 | chr10_43830659_43835596 chr01_11516392_11522733 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | ||||
DGAT1 | Diacylglycerol O-acyltransferase 1 | chr01_09730655_09741367 chr01_48275733_48286173 | Locus_14696 | chr05_08797620_08808333 | AT2G19450.1 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | Genome Assembly, Transcriptomics | [3,25] |
DGAT2 | Diacylglycerol O-acyltransferase 2 | chr14_26782964_26787941 chr18_25811826_25816791 | Locus_12629 | chr10_25785382_25790335 | AT3G51520.1 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | Genome Assembly, Transcriptomics | [3,25] |
DGAT3 | Diacylglycerol O-acyltransferase 3 | Locus_1560 | AT1G48300.1 | Acyl-CoA-dependent TAG synthesis in Kennedy pathway | Transcriptomics | [25] | ||
LPCAT | Lysophosphatidylcholine acyltransferase | chr01_06996630_07001595 chr05_56678891_56685081 chr01_03079195_03084058 chr07_53028425_53034567 chr01_43224061_43229071 chr02_66141068_66147271 chr02_04634020_04638876 chr19_35211932_35217537 | Locus_43749 | PC00000058_00436672_00441634 chr02_10454190_10460391 chr05_03185967_03190829 chr06_54113419_54119561 | AT1G12640.1 | PC-mediated TAG synthesis | Transcriptomics | [3,25] |
CPT1 | Diacylglycerol cholinephosphotransferase | Locus_7821 | AT1G13560.1 | PC-mediated TAG synthesis | Transcriptomics | [25] | ||
CPT2 | Diacylglycerol cholinephosphotransferase | Locus_22567 | AT3G25585.1 | PC-mediated TAG synthesis | Transcriptomics | [25] | ||
PDAT1 | Phospholipid:diacylglycerol acyltransferase 1 | chr05_44104376_44108847 | Locus_7255 | chr02_22969948_22974420 | AT5G13640.1 | Acyl-CoA independent pathway | Transcriptomics | [3,25] |
chr03_00447151_00451507 | PC00002899_00154872_00159184 | |||||||
chr02_52135886_52140327 | ||||||||
chr09_00376677_00380564 | ||||||||
PDAT2 | Phospholipid:diacylglycerol acyltransferase 2 | chr05_38922115_38924735 | Locus_29208 | chr02_28050267_28052887 | AT3G44830.1 | Acyl-CoA independent pathway | Transcriptomics | [3,25] |
chr02_45992086_45994691 | ||||||||
PDCT | Phosphatidylcholine:diacylglycerol cholinephosphotransferase | chr03_46291224_46293449 chr09_37050943_37053194 | Locus_15867 | chr01_27228085_27230144 | AT3G15820.1 | Acyl-CoA independent pathway | Genome Assembly, Transcriptomics | [3,25] |
OLEO2 | Oleosin2 | chr15_52133834_52134256 | Locus_31790 | AT5G40420.1 | TAG assembly | Transcriptomics | [3,25] | |
chr17_50355018_50355440 | chr09_02008310_02008732 | |||||||
OLEO | Oleosin | chr14_08347244_08347714 | Locus_31788 | chr10_44101965_44102435 | AT3G18570.1 | TAG assembly | Transcriptomics | [3,25] |
chr18_08871500_08871970 | ||||||||
OLEO1 | Oleosin1 | chr05_05196095_05196523 | Locus_29266 | chr02_64426568_64426996 | AT4G25140.1 | TAG assembly | Transcriptomics | [3,25] |
chr01_30156121_30156549 | ||||||||
OLEO5 | Oleosin5 | chr05_59989345_59989911 | Locus_29276 | AT3G01570.1 | TAG assembly | Transcriptomics | [3,25] | |
chr05_59997449_59997976 | chr02_07157257_07157823 | |||||||
chr02_69562819_69563393 | chr02_07149192_07149719 | |||||||
chr02_69577662_69578195 |
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Bae, S.-H.; Zoclanclounon, Y.A.B.; Kumar, T.S.; Oh, J.-H.; Lee, J.; Kim, T.-H.; Park, K.Y. Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update. Plants 2022, 11, 1207. https://doi.org/10.3390/plants11091207
Bae S-H, Zoclanclounon YAB, Kumar TS, Oh J-H, Lee J, Kim T-H, Park KY. Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update. Plants. 2022; 11(9):1207. https://doi.org/10.3390/plants11091207
Chicago/Turabian StyleBae, Seon-Hwa, Yedomon Ange Bovys Zoclanclounon, Thamilarasan Senthil Kumar, Jae-Hyeon Oh, Jundae Lee, Tae-Ho Kim, and Ki Young Park. 2022. "Advances in Understanding the Genetic Basis of Fatty Acids Biosynthesis in Perilla: An Update" Plants 11, no. 9: 1207. https://doi.org/10.3390/plants11091207