Detection and Simultaneous Differentiation of Three Co-infected Viruses in Zanthoxylum armatum
Abstract
:1. Introduction
2. Results
2.1. Validation of Specific Primers
2.2. Optimization of Multiplex RT-PCR
2.3. Sensitivity Detection of Multiplex RT-PCR
2.4. Detection of Field Samples by the Optimized Multiplex RT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Extraction of Total RNA and RT-PCR
4.3. PCR Amplification
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Viruses or Gene | Primers | Primer Sequences (5′-3′) | Fragment Length (bp) |
---|---|---|---|
GSPNeV | GSPNeV-5F | TGTCACTGAAGGAGCGGAT | 1039 |
GSPNeV-3R | ATCACCCATCTTAGCGACG | ||
GSPIV | GSPIV-5F | GATTAGGGCACACAGGAGTC | 730 |
GSPIV-3R | GCTATGGCTTCTTCACGAA | ||
GSPEV | GSPEV-5F | CTGGGTTATGGCAAACATC | 580 |
GSPEV-3R | AGGAGCCTCAGGAAGAATCT | ||
ITS2 | ITS2-5F | CGCATCGTTGCCCCAC | 224 |
ITS2-3R | CGATGCGAGCGCTGCTT |
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Dong, Z.; Zhao, X.; Liu, J.; Zhan, B.; Li, S. Detection and Simultaneous Differentiation of Three Co-infected Viruses in Zanthoxylum armatum. Plants 2022, 11, 1242. https://doi.org/10.3390/plants11091242
Dong Z, Zhao X, Liu J, Zhan B, Li S. Detection and Simultaneous Differentiation of Three Co-infected Viruses in Zanthoxylum armatum. Plants. 2022; 11(9):1242. https://doi.org/10.3390/plants11091242
Chicago/Turabian StyleDong, Zhenfei, Xiaoli Zhao, Junjie Liu, Binhui Zhan, and Shifang Li. 2022. "Detection and Simultaneous Differentiation of Three Co-infected Viruses in Zanthoxylum armatum" Plants 11, no. 9: 1242. https://doi.org/10.3390/plants11091242
APA StyleDong, Z., Zhao, X., Liu, J., Zhan, B., & Li, S. (2022). Detection and Simultaneous Differentiation of Three Co-infected Viruses in Zanthoxylum armatum. Plants, 11(9), 1242. https://doi.org/10.3390/plants11091242