Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in Scoparia dulcis
Abstract
:1. Introduction
2. Results
2.1. Cloning of Diterpene Synthases
2.2. Characterization of Enzymic Function of DTSs
2.3. Quantification of Scopadulcic Acid B (1) and Realtime qPCR Analysis of SdCPS2 and SdKSL1
3. Discussion
4. Material and Methods
4.1. Plant Material
4.2. Cloning of Diterpene Synthases
4.3. Construction of Expression Vectors
4.4. Heterologous Expression and Metabolite Analysis
4.5. Phylogenetic Analyses
4.6. Quantifation of Scopadulcic Acid B
4.7. Real-Time qPCR Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lee, J.-B.; Ohmura, T.; Yamamura, Y. Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in Scoparia dulcis. Plants 2023, 12, 69. https://doi.org/10.3390/plants12010069
Lee J-B, Ohmura T, Yamamura Y. Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in Scoparia dulcis. Plants. 2023; 12(1):69. https://doi.org/10.3390/plants12010069
Chicago/Turabian StyleLee, Jung-Bum, Tomoya Ohmura, and Yoshimi Yamamura. 2023. "Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in Scoparia dulcis" Plants 12, no. 1: 69. https://doi.org/10.3390/plants12010069