Comparative Microscopic, Transcriptome and IAA Content Analyses Reveal the Stem Growth Variations in Two Cultivars Ilex verticillata
Abstract
:1. Introduction
2. Results
2.1. Morphological and Histological Analysis of the Stem in ‘Oosterwijk’ and ‘Red Sprite’
2.2. Analysis of Differentially Expressed Genes
2.3. GO Classification and KEGG Analysis of Differentially Expressed Genes
2.4. Co-Expression Network Analysis of Genes in the Module
2.5. Construction of the Correlation Network of DEGs in the Module
2.6. Analysis of DEGs Related to the Auxin Signaling Pathway and Endogenous Auxin Content Determination
2.7. Analysis of Auxin Content Determination
3. Discussion
3.1. Water Transport Capacity of the ‘Oosterwijk’ Stem May Be Stronger Than ‘Red Sprite’
3.2. AST, HCT and bHLH94 May Be the Key Genes to the Regulation of Stem Growth
3.3. Auxin Affected the Growth and Development of the Stem
4. Materials and Methods
4.1. Plant Growth Conditions
4.2. Histological Analysis
4.3. RNA Isolation and Illumina Sequencing
4.4. De Novo Assembly and Functional
4.5. Identification of Differentially Expressed Genes
4.6. Weighted Gene Co-Expression Network Analysis
4.7. IAA Content Determination
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Qin, S.; Fu, S.; Yang, Y.; Sun, Q.; Wang, J.; Dong, Y.; Gu, X.; Wang, T.; Xie, X.; Mo, X.; et al. Comparative Microscopic, Transcriptome and IAA Content Analyses Reveal the Stem Growth Variations in Two Cultivars Ilex verticillata. Plants 2023, 12, 1941. https://doi.org/10.3390/plants12101941
Qin S, Fu S, Yang Y, Sun Q, Wang J, Dong Y, Gu X, Wang T, Xie X, Mo X, et al. Comparative Microscopic, Transcriptome and IAA Content Analyses Reveal the Stem Growth Variations in Two Cultivars Ilex verticillata. Plants. 2023; 12(10):1941. https://doi.org/10.3390/plants12101941
Chicago/Turabian StyleQin, Sini, Siyi Fu, Ying Yang, Qiumin Sun, Jingqi Wang, Yanling Dong, Xinyi Gu, Tao Wang, Xiaoting Xie, Xiaorong Mo, and et al. 2023. "Comparative Microscopic, Transcriptome and IAA Content Analyses Reveal the Stem Growth Variations in Two Cultivars Ilex verticillata" Plants 12, no. 10: 1941. https://doi.org/10.3390/plants12101941