Transcriptome and Metabolome Analysis of Selenium Treated Alfalfa Reveals Influence on Phenylpropanoid Biosynthesis to Enhance Growth
Abstract
:1. Introduction
2. Results
2.1. Effects of Na2SeO3 Spraying on Alfalfa Growth and Reproduction
2.2. Effects of Na2SeO3 Spraying on Physiological Changes of Alfalfa
2.3. Transcriptomic Analysis of Alfalfa Sprayed with Na2SeO3
2.4. Metabolomic Analysis of Alfalfa Sprayed with Na2SeO3
2.5. Integrative Analysis of Transcriptomics and Metabolomics Involved in Na2SeO3 Promotion of Alfalfa Growth
2.6. Characterization of the Phenylpropanoid Biosynthesis Pathway
3. Discussion
3.1. Effect of Growth and Reproductive Traits of Alfalfa
3.2. Effect of Physiological Changes of Alfalfa
3.3. Effect of Differentially Expressed Genes in Alfalfa
3.4. Effect of Differentially Metabolites of Alfalfa
3.5. Integrative Analysis of Transcriptomics and Metabolomics
4. Materials and Methods
4.1. Experimental Site
4.2. Plant Cultivation and Treatments
4.3. Determination of Growth and Reproductive Traits
4.4. Determination of Physiological Changes
4.5. RNA Extraction, Sequencing, and Functional Annotation
4.6. Validation of Transcriptomic Data
4.7. Metabolite Extraction and Metabolic Spectrum Analysis
4.8. Integrative Analysis
4.9. Bioinformatics Analysis of Related Genes
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Plant Height/cm | FW/g | DW/g | FW/DW% | Total Se/μg·g−1 |
---|---|---|---|---|---|
CK | 67.18 ± 2.17 | 2.06 ± 1.78 | 0.36 ± 0.02 b | 5.88 ± 0.29 a | 0.069 ± 0.04 b |
ST | 68.93 ± 1.98 | 2.40 ± 1.84 | 0.58 ± 0.03 a | 4.16 ± 0.29 b | 0.684 ± 0.11 a |
Treatment | Number of Florets | Amount of Pollen Per Flower | Pollen Viability% | Pod Spirals/Turns | Seed Numbers Per Pod | 1000-Seeds Weight/g |
---|---|---|---|---|---|---|
CK | 8.60 ± 0.37 | 23,160 ± 636.87 b | 45 ± 0.02 b | 2.87 ± 0.09 b | 4.93 ± 0.16 b | 2.46 ± 0.03 |
ST | 8.83 ± 0.30 | 33,160 ± 2219.37 a | 65 ± 0.05 a | 3.45 ± 0.08 a | 6.63 ± 029 a | 2.45 ± 0.03 |
Sample | Raw Reads | Raw Bases (G) | Valid Reads | Valid Base (G) | Valid% | Q20 (%) | Q30 (%) | GC (%) |
---|---|---|---|---|---|---|---|---|
CK1 | 50,673,258 | 7.60 | 49,766,778 | 7.47 | 98.21 | 99.98 | 97.75 | 42.50 |
CK2 | 49,465,606 | 7.42 | 48,475,862 | 7.27 | 98.00 | 99.99 | 97.75 | 42.00 |
CK3 | 43,909,580 | 6.59 | 43,074,702 | 6.46 | 98.10 | 99.99 | 97.53 | 41.50 |
ST1 | 48,251,932 | 7.24 | 47,412,748 | 7.11 | 98.26 | 99.99 | 97.66 | 42.50 |
ST2 | 46,300,900 | 6.95 | 45,163,196 | 6.77 | 97.54 | 99.99 | 97.62 | 42.00 |
ST3 | 52,649,430 | 7.90 | 51,661,074 | 7.75 | 98.12 | 99.99 | 97.68 | 42.00 |
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Wang, F.; Yang, J.; Hua, Y.; Wang, K.; Guo, Y.; Lu, Y.; Zhu, S.; Zhang, P.; Hu, G. Transcriptome and Metabolome Analysis of Selenium Treated Alfalfa Reveals Influence on Phenylpropanoid Biosynthesis to Enhance Growth. Plants 2023, 12, 2038. https://doi.org/10.3390/plants12102038
Wang F, Yang J, Hua Y, Wang K, Guo Y, Lu Y, Zhu S, Zhang P, Hu G. Transcriptome and Metabolome Analysis of Selenium Treated Alfalfa Reveals Influence on Phenylpropanoid Biosynthesis to Enhance Growth. Plants. 2023; 12(10):2038. https://doi.org/10.3390/plants12102038
Chicago/Turabian StyleWang, Fengdan, Jie Yang, Yi Hua, Kexin Wang, Yue Guo, Yalin Lu, Siqi Zhu, Pan Zhang, and Guofu Hu. 2023. "Transcriptome and Metabolome Analysis of Selenium Treated Alfalfa Reveals Influence on Phenylpropanoid Biosynthesis to Enhance Growth" Plants 12, no. 10: 2038. https://doi.org/10.3390/plants12102038