Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought
Abstract
:1. Introduction
2. Results
2.1. Options of Different Concentrations of Proline to Improve RWC and Growth Recovery of Drought-Stressed Oilseed Rape
2.2. Impact of Proline Application on Morphometric Parameters of Oilseed Rape Seedlings Exposed to Drought
2.3. Impact of Proline Application on RWC of Prolonged Drought-Stressed Oilseed Rape Leaves
2.4. Impact of Proline Application on Chlorophyll Content of Drought-Stressed Oilseed Rape Leaves
2.5. Impact of Proline Application on Ethylene Emission of Drought-Stressed Oilseed Rape Leaves
2.6. Impact of Proline Application on H2O2 Levels of Drought-Stressed Oilseed Rape Leaves
2.7. Impact of Proline Application on MDA Content of Drought-Stressed Oilseed Rape Leaves
2.8. Effects of Exogenous Proline on PM ATPase Activity of Rapeseed Seedlings Exposed to Drought
2.9. Effect of Exogenous Proline on Endogenous Proline Content of Rapeseed Seedlings Exposed to Drought
2.10. Effect of Exogenous Proline on Survival of Plants
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Treatments
4.3. Determination of the Active Proline Concentration
4.4. Experimental Design of Drought-Stress Control Studies
4.5. Sampling
4.6. Morphometrical Measurements
4.7. Recovery Evaluation
4.8. Survival Evaluation
4.9. Relative Water Content (RWC)
4.10. Assessment of Biochemical Parameters
4.10.1. Photosynthetic Pigments
4.10.2. Ethylene
4.10.3. Hydrogen Peroxide (H2O2)
4.10.4. MDA Content
4.10.5. H+-ATPase Activity Assay
4.10.6. Proline
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment (3 mL) | RWC, % | |
---|---|---|
4 Days | 8 Days | |
Control, H2O | 82.3 a | 82.5 a |
Proline 0.1 mM | 81.5 a | 81.7 a |
Proline 1 mM | 82.3 a | 80.7 a |
Proline 10 mM | 84.0 a | 85.6 a |
Drought | 70.1 c | 63.2 d |
Proline 0.1 mM + Drought | 74.7 b | 58.8 e |
Proline 1 mM + Drought | 73.6 b | 70.7 c |
Proline 10 mM + Drought | 70.9 c | 59.8 f |
Treatment (12.5 mL) | ||
Control, H2O | 84.2 a | 83.5 a |
Proline 0.1 mM | 85.2 a | 87.2 a |
Proline 1 mM | 80.7 a | 87.3 a |
Proline 10 mM | 80.1 a | 86.8 a |
Drought | 63.1 d | 51.9 e |
Proline 0.1 mM + Drought | 73.7 b | 60.1 d |
Proline 1 mM + Drought | 74.6 b | 72.6 bc |
Proline 10 mM + Drought | 70.4 c | 58.7 d |
Treatment (3 mL) | Average Weight (g) | |
---|---|---|
Fresh | Dry | |
Control, H2O | 0.91 ab | 0.051 a |
Proline 0.1 mM | 0.88 b | 0.052 a |
Proline 1 mM | 1.01 a | 0.055 a |
Proline 10 mM | 1.24 a | 0.052 a |
Drought | 0.84 c | 0.041 c |
Proline 0.1 mM + Drought | 0.75 cd | 0.045 ab |
Proline 1 mM + Drought | 0.78 c | 0.049 ab |
Proline 10 mM + Drought | 0.97 ab | 0.044 b |
Treatment (12.5 mL) | ||
Control, H2O | 1.13 a | 0.057 a |
Proline 0.1 mM | 1.26 a | 0.056 a |
Proline 1 mM | 1.47 a | 0.059 a |
Proline 10 mM | 1.35 a | 0.057 a |
Drought | 1.02 b | 0.043 c |
Proline 0.1 mM + Drought | 1.07 ab | 0.043 c |
Proline 1 mM + Drought | 1.18 a | 0.055 ab |
Proline 10 mM + Drought | 1.11 a | 0.051 b |
Treatment | Average Length (cm) | Average Weight (g) | |||||||
---|---|---|---|---|---|---|---|---|---|
Fresh | Dry | ||||||||
4 Days | 8 Days | 4 Days Recovery | 4 Days | 8 Days | 4 Days Recovery | 4 Days | 8 Days | 4 Days Recovery | |
Control H2O | 15.26 a | 17.27 a | 18.00 b | 0.68 b | 0.74 b | 0.77 b | 0.045 a | 0.045 a | 0.046 a |
Proline | 15.76 a | 17.93 a | 19.03 a | 0.74 a | 0.87 a | 0.89 a | 0.048 a | 0.048 a | 0.049 a |
Drought | 14.64 b | 14.74 c | 15.71 c | 0.51 c | 0.45 d | 0.46 c | 0.030 c | 0.041 b | 0.038 b |
Proline + Drought | 14.89 ab | 15.29 b | 17.75 b | 0.67 b | 0.49 c | 0.70 b | 0.036 b | 0.047 a | 0.039 b |
Treatment | Chlorophyll Contents (mg g−1 FW) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Chlorophyll a | Chlorophyll b | Chlorophyll a + b | |||||||
4 Days | 8 Days | 4 Days Recovery | 4 Days | 8 Days | 4 Days Recovery | 4 Days | 8 Days | 4 Days Recovery | |
Control, H2O | 0.98 a | 0.99 a | 0.85 a | 0.24 a | 0.23 a | 0.24 a | 1.22 a | 1.22 a | 1.09 a |
Proline | 0.97 a | 1.02 a | 0.85 a | 0.25 a | 0.24 a | 0.25 a | 1.22 a | 1.23 a | 1.11 a |
Drought | 0.78 c | 0.57 c | 0.67 b | 0.21 a | 0.12 b | 0.18 b | 0.98 c | 0.69 c | 0.85 b |
Proline + Drought | 0.93 b | 0.62 b | 0.89 a | 0.21 a | 0.13 b | 0.22 a | 1.13 b | 0.75 b | 1.10 a |
Treatment | Number of Survived Plants (%) |
---|---|
Control H2O | 100.00 a |
Proline | 100.00 a |
Drought | 19.79 ± 2.21 b |
Proline + Drought | 44.10 ± 3.15 c |
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Jurkonienė, S.; Mockevičiūtė, R.; Gavelienė, V.; Šveikauskas, V.; Zareyan, M.; Jankovska-Bortkevič, E.; Jankauskienė, J.; Žalnierius, T.; Kozeko, L. Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought. Plants 2023, 12, 2718. https://doi.org/10.3390/plants12142718
Jurkonienė S, Mockevičiūtė R, Gavelienė V, Šveikauskas V, Zareyan M, Jankovska-Bortkevič E, Jankauskienė J, Žalnierius T, Kozeko L. Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought. Plants. 2023; 12(14):2718. https://doi.org/10.3390/plants12142718
Chicago/Turabian StyleJurkonienė, Sigita, Rima Mockevičiūtė, Virgilija Gavelienė, Vaidevutis Šveikauskas, Mariam Zareyan, Elžbieta Jankovska-Bortkevič, Jurga Jankauskienė, Tautvydas Žalnierius, and Liudmyla Kozeko. 2023. "Proline Enhances Resistance and Recovery of Oilseed Rape after a Simulated Prolonged Drought" Plants 12, no. 14: 2718. https://doi.org/10.3390/plants12142718