Chromosome Rearrangement in Elymus dahuricus Revealed by ND-FISH and Oligo-FISH Painting
Abstract
:1. Introduction
2. Results
2.1. Genome Identification Using Combined FISH and GISH Analyses
2.2. Precise Chromosome Identification by ND-FISH
2.3. Identification of Chromosome Linkage Groups
2.4. Establishment of Universal Karyotyping Nomenclature System
2.5. Comparison Analysis of Centromere Specific Repeat Sequences and CENH3
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Chromosome Preparation and ND-FISH Analysis
4.3. GISH and Oligo-FISH Painting
4.4. Chromosomal Immunolocalization
4.5. Drawing Karyotype Ideogram
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oligo Probes | Sequences | Reference |
---|---|---|
Oligo-pTa535 | AAAAACTTGACGCACGTCACGTACAAATTGGACAAACTCTTTCGGAGTATCAGGGTTTC | [24] |
Oligo-pSc119.2 | CCGTTTTGTGGACTATTACTCACCGCTTTGGGGTCCCATAGCTAT | [24] |
Oligo-5SrDNA | TCAGAACTCCGAAGTTAAGCGTGCTTGGGCGAGAGTAGTAC | [23] |
Oligo-18SrDNA | GGGCAAGTCTGGTGCCAGCAGCCGCGGT | [23] |
Oligo-7E-744 | GCCACCGTGCAGTAGACTTTTTTTGTACCCAAACCATCAGTAACAAAGTTCGTTCAC | [25] |
Oligo-3A1 | AATAATTTTACACTAGAGTTGAACTAGCTCTATAAGCTAGTTCA | [26] |
Oligo-(GAA)7 | GAAGAAGAAGAAGAAGAAGAA | [27] |
Oligo-HvCSR | ACAACGACAACAACGACAATGACGAGA | This study |
Oligo-7E-716 | GTACAGGACTGCAGCTAAGCCCCCGAGTGAGAGGGTTGCTCATCACTCGGTAGGATT | This study |
Oligo-7E-599 | CATCGGTCAAACCTCGTCCGGCGAAAGTCAAAGGCGTAGACCGCCCGGTCAACGGTGCC | This study |
Oligo-P05 | AATACGCTCTTGTTCTTGGCTGTCACGCACATACTTTATGGGATGTCATAGG | This study |
Oligo-13-J1011 | CATCATGCTTGTTGTGAGAAGCTCTGGTTTGTGAGAAGCATATACCCAAACC | This study |
Oligo-D01-135 | ACGCGCGCCATGGAAAACAGGGCAAAACCACCGACTCGTCCACGACTCGTAC | This study |
Oligo-Ae334 | CTCCAAAGTGTTCCTATGGGCTGACCTAACACAACCGGGTGG | This study |
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Jiang, C.; Liu, X.; Yang, Z.; Li, G. Chromosome Rearrangement in Elymus dahuricus Revealed by ND-FISH and Oligo-FISH Painting. Plants 2023, 12, 3268. https://doi.org/10.3390/plants12183268
Jiang C, Liu X, Yang Z, Li G. Chromosome Rearrangement in Elymus dahuricus Revealed by ND-FISH and Oligo-FISH Painting. Plants. 2023; 12(18):3268. https://doi.org/10.3390/plants12183268
Chicago/Turabian StyleJiang, Chengzhi, Xiaodan Liu, Zujun Yang, and Guangrong Li. 2023. "Chromosome Rearrangement in Elymus dahuricus Revealed by ND-FISH and Oligo-FISH Painting" Plants 12, no. 18: 3268. https://doi.org/10.3390/plants12183268