Comparative Physiological and Transcriptome Analyses Reveal Mechanisms of Salicylic-Acid-Reduced Postharvest Ripening in ‘Hosui’ Pears (Pyrus pyrifolia Nakai)
Abstract
:1. Introduction
2. Results
2.1. Effect of SA Treatment on the Postharvest Ripening Process of Pear Fruit
2.2. Effect of SA Treatment on the Cell Wall Composition of Pear Fruit during Room-Temperature Storage
2.3. Effect of SA Treatment on the Soluble Sugar Composition of Pear Fruit during Room-Temperature Storage
2.4. Characterization of Transcriptional Changes of Pear Fruit in Response to Salicylic Acid by RNA-Seq
2.5. DEGs Related to Plant Hormone Signal Transduction
2.6. DEGs Related to Sugar Metabolism
2.7. DEGs Related to Fruit Softening
2.8. Differentially Expressed TFs
2.9. Validation of RNA-Seq by qRT-PCR Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Material and Treatments
4.2. Weight and Firmness
4.3. Determination of Total Phenols and Ascorbic Acid
4.4. Determination of Cell Wall Components
4.5. Determination of Sugar Components
4.6. RNA Extraction and RNA-Seq
4.7. cDNA Synthesis and qRT-PCR Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Zhang, J.; Wen, M.; Dai, R.; Liu, X.; Wang, C. Comparative Physiological and Transcriptome Analyses Reveal Mechanisms of Salicylic-Acid-Reduced Postharvest Ripening in ‘Hosui’ Pears (Pyrus pyrifolia Nakai). Plants 2023, 12, 3429. https://doi.org/10.3390/plants12193429
Zhang J, Wen M, Dai R, Liu X, Wang C. Comparative Physiological and Transcriptome Analyses Reveal Mechanisms of Salicylic-Acid-Reduced Postharvest Ripening in ‘Hosui’ Pears (Pyrus pyrifolia Nakai). Plants. 2023; 12(19):3429. https://doi.org/10.3390/plants12193429
Chicago/Turabian StyleZhang, Jing, Mengmeng Wen, Rong Dai, Xiao Liu, and Chunlei Wang. 2023. "Comparative Physiological and Transcriptome Analyses Reveal Mechanisms of Salicylic-Acid-Reduced Postharvest Ripening in ‘Hosui’ Pears (Pyrus pyrifolia Nakai)" Plants 12, no. 19: 3429. https://doi.org/10.3390/plants12193429