Quantitative Proteomic Analysis Deciphers the Molecular Mechanism for Endosperm Nuclear Division in Early Rice Seed Development
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsReviewer's comments on the manuscript «Quantitative proteomic analysis deciphers the molecular mechanism for endosperm nuclear division in early rice seed development».
I want to note right away that an important and interesting problems about the molecular mechanism of early seed development and, in this regard, about grain yield and quality of crop plants are being raised and investigated.
Of course, the data obtained are worthy of publication in the «Plants». However, I have a few comments.
1. In the abstract you write (line 21):
…free quantitative proteomic analysis of developing rice seeds of the WT and osctps1-2 mutant, which…
It would be good to indicate immediately after the first mention of osctps1-2 mutant in parentheses (mutations in OsCTPS1 that encodes a cytidine triphosphate synthase (CTPS)).
2. Lines 73–75:
Both OsMADS78 and OsMADS79 interact with another type I MADS-box protein, OsMADS89, which enhances their nuclear localization of OsMADS78 and OsMADS79 [22].
More correct:
Both OsMADS78 and OsMADS79 interact with another type I MADS-box protein, OsMADS89, which enhances their nuclear localization [22].
3. Lines 211–212 onwards:
…we isolated protoplasts from OsCTPS1-GFP overexpressing transgenic plants in 10-day-old seedlings and examined the stability of the OsCTPS1-sGFP protein…
Should check should it be OsCTPS1-GFP or OsCTPS1-sGFP?
Author Response
"Please see the attachment."
Reviewer 2 Report
Comments and Suggestions for AuthorsThe paper provides valuable information on protein content during endosperm development in rice. I have only a few minor comments and suggestions.
1. I don’t see any statistics in Figure 1C.
2. What are the green and dark green bars in Figure 6E? Please mention in the legends.
Aside from this, I don't see any big issues with writing, experimental design, or data analysis. Everything appears to be in order. I'd like to advise that the author cite this paper while talking about endosperm:
Molecular and Transcriptional Regulation of Seed Development in Cereals: Present Status and Future Prospects
Author Response
Please see the attachment
Author Response File: Author Response.pdf