Content and Yield of L-DOPA and Bioactive Compounds of Broad Bean Plants: Antioxidant and Anti-Inflammatory Activity In Vitro

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The authors in this manuscript entitled “Content and yield of L-DOPA and bioactive compounds of broad bean plants: antioxidant and anti-inflammatory activity in vitro” studied the contents and yield of chemical components specially L-DOPA, rutin, and isoorientins and the chemical classes for instance flavonoids, phenolics etc. from broad bean plant.

Careful Analyses of this research suggested following revisions:

Lack of details: How the authors confirmed the enantiomeric purity of L-DOPA.

Lack of experiments: why authors did not isolate the active constituents specially L-DOPA, rutin, and isoorientins from the plant. Isolation and characterization of these bioactive compounds using modern isolation and characterization techniques are lacking from the manuscript.

Chemical structures of bioactive compounds:  Authors should provide the chemical structures of the identified bioactive compounds from the plant in the manuscript.

-HPLC Chromatograms are blurred and must be improved.

-In chromatograms, authors should mention the name of the identified compounds for their respective peak.

- Lack of Conclusion: The conclusion does not summarize the deliverables or the implications of the results. It is essential to provide a synthesis of the key perspectives and their significance.

-There are English grammar and typographical mistakes throughout the manuscript which must be rectified.

Comments on the Quality of English Language

Moderate editing of English language required

Author Response

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Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The paper by Delgado-Alvarado and coworkers describes the content and yield of total phenolic compounds, toral flavonoids, and L-DOPA of four Mexican broad bean varieties, the flavonoid profile in the plant extract, and the antioxidant and anti-inflammatory activity in vitro. The experimental procedures have been carried out in a reliable way and the interpretations offered are all very reasonable. There are only a few minor points listed below:

1. Error bars would be needed for Figure 6 and 7.

2. I think that the section 4 (The broad bean plant as a source of L-DOPA for Parkinson’s disease patients) is too long and should be more concise. This section would be included into the section 3 as a discussion.

Author Response

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Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

Authors have responded to all queries.

Comments on the Quality of English Language

Minor editing of English language required

Author Response

Please see the attachment

Author Response File: Author Response.pdf