LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
Abstract
:1. Introduction
2. Results
2.1. Non-Targeted Screening of the Latex Chloroform Extracts Using Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry Employing an Electrospray Ionization Source
2.2. Examination of the Anticancer Activity of the ES and EC Latex Extracts
3. Discussion
3.1. Non-Targeted Screening of the Latex Chloroform Extracts Using Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry Employing an Electrospray Ionization Source
3.2. Examination of the Anticancer Activity of the ES and EC Latex Extracts
4. Materials and Methods
4.1. Plant Materials
4.2. Chemicals
4.3. Sample Preparation and Liquid Chromatography-Electrospray Quadrupole Time-of-Flight Mass Spectrometry (LC-ESI QTOF MS) Measurements
4.4. Drugs
4.5. Cells and Cell Culture
4.6. Cell Viability Assay
4.7. Rhodamine 123 Accumulation Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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No. | RT (min) | Ion Species | m/z Measured | Molecular Mass Measured | Proposed Formula | Molecular Mass Calculated | Diff. (ppm) | Compound (CAS No.) [Ref.] | Class |
---|---|---|---|---|---|---|---|---|---|
1 | 5.26 | [M+H]+ [M+Na]+ [M+K]+ | 720.3018 742.2834 758.2570 | 719.2944 | C39H45NO12 | 719.2942 | 0.29 | 2595240-63-2 [41] 2595235-60-0 [41] 2595235-05-3 [41] 2595233-36-4 [41] 777896-12-5 [42] 2408424-43-9 [43] 2342577-75-5 [44] | Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Premyrsinane Premyrsinane |
2 | 6.27 | [M+H]+ [M+Na]+ [M+K]+ | 734.3173 756.2992 772.2729 | 733.3155 | C40H47NO12 | 733.3098 | 2.28 | 1615711-25-5 [45] 1380589-96-7 [44,46,47] 2112824-86-7 [48] 1980015-12-0 [49] 1778734-87-4 [50] | Tetrahydroingenoid Premyrsinane Premyrsinane Premyrsinane Premyrsinane |
3 * | 6.49 | [M+H]+ [M+Na]+ [M+K]+ | 750.3124 772.2927 788.2673 | 749.3039 | C40H47NO13 | 749.3047 | −1.11 | / | / |
4 | 6.52 | [M+H]+ [M+NH4]+ [M+Na]+ | 717.3019 734.3173 739.2826 | 716.2928 | C39H44N2O11 | 716.2945 | −2.35 | 171864-09-8 [14,46] | Myrsinane |
5 * | 7.07 | [M+H]+ [M+NH4]+ | 637.2615 654.2910 | 636.2570 | C35H40O11 | 636.2571 | −0.04 | / | / |
6 * | 7.11 | [M+H]+ [M+Na]+ [M+K]+ | 782.3168 804.2986 820.2721 | 781.3094 | C44H47NO12 | 781.3098 | −0.54 | / | / |
7 * | 7.12 | [M+H]+ [M+Na]+ [M+K]+ | 671.3060 693.2894 709.2617 | 670.2991 | C36H46O12 | 670.2989 | 0.26 | 247099-10-1 [44,51,52,53] | Premyrsinane |
8 * | 7.24 | [M+H]+ [M+Na]+ [M+K]+ | 790.3072 812.2888 828.2625 | 789.2997 | C42H47NO14 | 789.2997 | 0.12 | 1380590-01-1 [46] | Cyclomyrsinane |
9 * | 7.28 | [M+H]+ [M+NH4]+ | 654.2911 671.3058 | 653.2837 | C35H43NO11 | 653.2836 | 0.14 | 171864-14-5 [14,54,55] 1799735-20-8 [56] | Myrsinane Myrsinane |
10 | 7.33 | [M+H]+ [M+Na]+ [M+K]+ | 748.3331 770.3146 786.2883 | 747.3256 | C41H49NO12 | 747.3255 | 0.17 | 1928726-37-7 [47,57] 1380589-97-8 [46,47] | Premyrsinane Premyrsinane |
11 | 7.67 | [M+H]+ [M+Na]+ [M+K]+ | 734.3175 756.2991 772.2719 | 733.3101 | C40H47NO12 | 733.3098 | 0.40 | 1615711-25-5 [45] 1380589-96-7 [44,46,47] 2112824-86-7 [48] 1980015-12-0 [49] 1778734-87-4 [50] | Ingenoid Premyrsinane Premyrsinane Premyrsinane Premyrsinane |
12 | 8.12 | [M+H]+ [M+Na]+ | 656.3064 678.2878 | 655.2991 | C35H45NO11 | 655.2993 | −0.32 | 2222920-06-9 [58] | Jatrophane |
13 | 8.39 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 671.3063 688.3329 693.2884 709.2621 1363.5871 | 670.2990 | C36H46O12 | 670.2989 | 0.15 | 247099-10-1 [44,51,52,53] | Premyrsinane |
14 * | 8.77 | [M+H]+ [M+Na]+ [M+K]+ | 748.3332 770.3149 786.2886 | 747.3257 | C41H49NO12 | 747.3255 | 0.37 | 1928726-37-7 [47,57] 1380589-97-8 [46,47] | Premyrsinane Premyrsinane |
15 | 9.08 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ [2M+K]+ | 629.2948 646.3220 651.2777 667.2511 1279.5629 1295.5367 | 628.2882 | C34H44O11 | 628.2884 | −0.23 | 2112824-87-8 [48] 1801541-77-4 [53] | Premyrsinane Premyrsinane |
16 * | 9.34 | [M+H]+ [M+Na]+ [M+K]+ | 704.3074 726.2892 742.2619 | 703.2999 | C39H45NO11 | 703.2993 | 0.87 | 1529776-07-5 [59] 777896-21-6 [42] | Premyrsinane Jatrophane |
17 | 9.36 | [M+H]+ [M+NH4]+ [M+Na]+ | 716.3066 733.3201 738.2873 | 715.2993 | C40H45NO11 | 715.2993 | 0.07 | 171864-12-3 [14,46,52,60] | Myrsinane |
18 | 9.62 | [M+H]+ [M+Na]+ [M+K]+ | 733.3221 755.3040 771.2779 | 732.3147 | C41H48O12 | 732.3146 | 0.15 | 2674753-70-7 [61] | Premyrsinane |
19 | 10.21 | [M+H]+ [M+Na]+ [M+K]+ [2M+Na]+ | 718.3225 740.3040 756.2777 1457.6217 | 717.3151 | C40H47NO11 | 717.3149 | 0.31 | / | / |
20 | 10.43 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 591.2949 608.3219 613.2775 629.2511 1203.5657 | 590.2881 | C35H42O8 | 590.2880 | 0.30 | 1809418-89-0 [62,63] | Lathyrane |
21 | 10.57 | [M+H]+ [M+Na]+ [M+K]+ | 744.3382 766.3201 782.2932 | 743.3308 | C42H49NO11 | 743.3306 | 0.29 | / | / |
22 | 10.69 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 733.3217 750.3487 755.3042 771.2778 | 732.3148 | C41H48O12 | 732.3146 | 0.35 | 2674753-70-7 [61] | Premyrsinane |
23 | 10.73 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 672.3380 677.2964 693.2670 1331.5980 | 654.3057 | C36H46O11 | 654.3040 | 2.64 | 1335200-98-0 [52] 1333481-71-2 [64,65] 173967-58-3 [66] | Premyrsinane Premyrsinane Premyrsinane |
24 * | 11.06 | [M+NH4]+ [M+Na]+ [M+K]+ | 698.3534 703.3089 719.2825 | 680.3197 | C38H48O11 | 680.3197 | 0.09 | 1946844-21-8 [67] | Jatrophane |
25 * | 11.95 | [M+NH4]+ [M+Na]+ [M+K]+ | 720.3378 725.2934 741.2664 | 702.3040 | C40H46O11 | 702.3040 | 0.00 | / | / |
26 | 12.70 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 734.3536 739.3092 755.2826 1455.6305 | 716.3199 | C41H48O11 | 716.3197 | 0.29 | 1529776-06-4 [59] | Premyrsinane |
27 * | 12.89 | [M+NH4]+ [M+Na]+ [M+K]+ | 760.3690 765.3247 781.2977 | 742.3353 | C43H50O11 | 742.3353 | −0.05 | / | / |
28 * | 13.01 | [M+NH4]+ [M+Na]+ [M+K]+ | 668.3791 673.3347 689.3083 | 650.3454 | C38H50O9 | 650.3455 | −0.14 | 72826-62-1 [68,69] | Tigliane |
29 | 13.92 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+NH4]+ [2M+Na]+ | 611.3574 628.3842 633.3397 649.3132 1238.7358 1243.6920 | 610.3505 | C36H50O8 | 610.3506 | -0.10 | 57672-63-6 [70,71,72,73] | Tigliane |
30 | 14.13 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 611.3575 628.3843 633.3399 649.3134 1243.6861 | 610.3508 | C36H50O8 | 610.3506 | 0.36 | 57672-63-6 [70,71,72,73] | Tigliane |
31 | 17.78 | [M+H]+ [M+Na]+ [M+K]+ [2M+Na]+ | 371.3156 393.2977 409.2714 763.6054 | 370.3085 | C22H42O4 | 370.3083 | 0.38 | / | / |
No. | RT (min) | Ion Species | m/z Measured | Molecular Mass Measured | Proposed Formula | Molecular Mass Calculated | Diff. (ppm) | Compound (CAS No.) [Ref.] | Class |
---|---|---|---|---|---|---|---|---|---|
32 | 1.31 | [M+H]+ | 146.1177 | 145.1104 | C7H15NO2 | 145.1103 | 0.77 | 407-64-7 [74] 1115-90-8 [75] | Amino acid Amino acid |
33 | 3.33 | [M+NH4]+ [M+Na]+ [M+K]+ | 648.3012 653.2569 669.2304 | 630.2676 | C33H42O12 | 630.2676 | −0.09 | 1811547-09-7 [76,77] 2049749-80-4 [78] | Jatrophane ent-Atisane |
34 * | 4.03 | [M+NH4]+ [M+Na]+ [M+K]+ | 668.3059 673.2617 689.2357 | 650.2725 | C36H42O11 | 650.2777 | −0.32 | 1254956-17-6 [79,80,81] 1210299-33-4 [81] 2002494-82-6 [82] | Daphnane Daphnane Daphnane |
35 * | 4.15 | [M+NH4]+ [M+Na]+ [M+K]+ | 588.2799 593.2356 609.2093 | 570.2463 | C31H38O10 | 570.2465 | −0.36 | 313486-57-6 [83] 313486-56-5 [83] 100288-19-5 [84] 2758418-28-7 [85] 2347529-35-3 [86] 1974283-21-0 [87] | Myrsinane Myrsinane Jatrophane Jatrophane Jatrophane Paraliane |
36 * | 5.27 | [M+H]+ [M+Na]+ [M+K]+ | 676.2749 698.2570 714.2307 | 675.2676 | C37H41NO11 | 675.2680 | −0.50 | 2685765-74-4 [88] 2685765-73-3 [88] 2685762-55-2 [88] | Ingol Ingol Ingol |
37 | 5.51 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 630.2909 635.2462 651.2199 1247.5024 | 612.2570 | C33H40O11 | 612.2571 | −0.18 | 780755-68-2 [89,90] 709002-56-2 [90,91] 566189-66-0 [86,92] 2347529-24-0 [86,93] 2347529-23-9 [86] 220705-94-2 [94,95] 371974-77-5 [95] 313486-55-4 [83] 212842-87-0 [96] 557104-67-3 [97] 608525-82-2 [98] 616217-04-0 [99] 89984-07-6 [100] 2803346-38-3 [101] | Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Lathyrane Lathyrane Lathyrane Lathyrane Myrsinol Myrsinane Myrsinane Ingol Ingol |
38 | 5.94 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 710.3168 715.2726 731.2464 1407.5551 | 692.2832 | C38H44O12 | 692.2833 | −0.11 | 100198-29-6 [102] 100198-28-5 [102] 2051585-34-1 [77,103] 2051585-29-4 [103] | Jatrophane Jatrophane Jatrophane Jatrophane |
39 | 5.97 | [M+NH4]+ [M+Na]+ [M+K]+ | 754.3429 759.2986 775.2723 | 736.3093 | C40H48O13 | 736.7395 | −0.26 | / | / |
40 | 6.17 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 710.3168 715.2722 731.2462 1407.5558 | 692.2830 | C38H44O12 | 692.2833 | −0.44 | 100198-29-6 [102] 100198-28-5 [102] 2051585-34-1 [77,103] 2051585-29-4 [103] | Jatrophane Jatrophane Jatrophane Jatrophane |
41 | 6.42 | [M+NH4]+ [M+Na]+ [M+K]+ | 690.3483 695.3040 711.2775 | 672.3147 | C36H48O12 | 672.3146 | 0.14 | / | / |
42 | 6.95 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 650.2952 655.2513 671.2250 1287.5141 | 632.2620 | C36H40O10 | 632.2621 | −0.20 | 2561483-25-6 [104] | Lathyrane |
43 * | 7.76 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 720.3016 737.3355 742.2842 758.2579 | 719.2942 | C39H45NO12 | 719.2942 | 0.37 | 2595240-63-2 [41] 2595235-60-0 [41] 2595235-05-3 [41] 2595233-36-4 [41] 777896-12-5 [42] 2408424-43-9 [43] 2342577-75-5 [44] | Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Premyrsinane Premyrsinane |
44 * | 7.88 | [M+NH4]+ [M+Na]+ [M+K]+ | 692.3067 697.2621 713.2401 | 674.2734 | C38H42O11 | 674.2727 | 0.97 | 2685775-35-1 [88,101] 2685775-67-9 [88] 2750352-31-7 [105] 2347529-31-9 [86] | Ingol Ingol Ingol Jatrophane |
45 * | 8.37 | [M+NH4]+ [M+Na]+ [M+K]+ | 752.3276 757.2829 773.2562 | 734.2937 | C40H46O13 | 734.2938 | −0.23 | 2051585-33-0 [77,103] 2891708-35-1 [106] | Jatrophane Jatrophane |
46 * | 8.60 | [M+NH4]+ [M+Na]+ [M+K]+ | 844.3174 849.2731 865.2466 | 826.2837 | C45H46O15 | 826.2837 | −0.01 | 2595253-64-6 [41] | Jatrophane |
47 | 8.70 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ [2M+K]+ | 675.2774 692.3069 697.2625 713.2437 1371.5372 1387.5098 | 674.2747 | C38H42O11 | 674.2727 | 2.97 | 2685775-35-1 [88,101] 2685775-67-9 [88] 2750352-31-7 [105] 2347529-31-9 [86] | Ingol Ingol Ingol Jatrophane |
48 | 9.26 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 717.2887 734.3177 739.2728 755.2465 | 716.2837 | C40H44O12 | 716.2833 | 0.53 | 2685775-23-7 [88] 2685765-76-6 [88] 2347529-37-5 [86] 2347529-36-4 [86] 1342887-24-4 [93,107] | Ingol Ingol Jatrophane Jatrophane Jatrophane |
49 * | 9.36 | [M+NH4]+ [M+Na]+ [M+K]+ | 812.3277 817.2833 833.2617 | 794.2944 | C45H46O13 | 794.2938 | 0.71 | / | / |
50 | 9.47 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 734.3173 739.2728 755.2576 1455.5590 | 716.2843 | C40H44O12 | 716.2833 | 1.36 | 2685775-23-7 [88] 2685765-76-6 [88] 2347529-37-5 [86] 2347529-36-4 [86] 1342887-24-4 [93,107] | Ingol Ingol Jatrophane Jatrophane Jatrophane |
51 | 9.66 | [M+NH4]+ [M+Na]+ [M+K]+ [2M+Na]+ | 676.3117 681.2672 697.2416 1339.5422 | 658.2779 | C38H42O10 | 658.2778 | 0.19 | 2366129-51-1 [60] 2366129-44-2 [60] 2750352-32-8 [105] 1613699-93-6 [108] 1151831-79-6 [109] | Myrsinane Myrsinane Ingol Ingol Ingol |
52 | 9.70 | [M+NH4]+ [M+Na]+ [M+K]+ | 856.3539 861.3090 877.2825 | 838.3198 | C47H50O14 | 838.3201 | −0.29 | / | / |
53 | 10.02 | [M+Na]+ [M+K]+ [2M+Na]+ | 579.2356 595.2091 1135.4805 | 556.2463 | C34H36O7 | 556.2461 | 0.32 | 59086-90-7 [110] 91413-70-6 [111] 91413-69-3 [111] 174389-91-4 [112] 92118-01-9 [113] | Ingenane Ingenane Ingenane Ingenane Tigliane |
54 | 10.19 | [M+NH4]+ [M+Na]+ [M+K]+ | 714.3484 719.3038 735.2776 | 696.3145 | C38H48O12 | 696.3146 | −0.07 | 284666-41-7 [114] 606136-90-7 [115] 1977558-48-7 [57] | Jatrophane Jatrophane Myrsinane |
55 * | 10.30 | [M+NH4]+ [M+Na]+ [M+K]+ | 772.3330 777.2879 793.2610 | 754.2985 | C43H46O12 | 754.2989 | −0.03 | 1449465-16-0 [80] | Daphnane |
56 | 10.33 | [M+NH4]+ [M+Na]+ [M+K]+ | 714.3486 719.3039 735.2774 | 696.3147 | C38H48O12 | 696.3146 | 0.14 | 284666-41-7 [114] 606136-90-7 [115] 1977558-48-7 [57] | Jatrophane Jatrophane Myrsinane |
57 | 10.43 | [M+NH4]+ [M+Na]+ [M+K]+ | 760.3326 765.2876 781.2606 | 742.2985 | C42H46O12 | 742.2989 | −0.52 | / | / |
58 | 10.47 | [M+NH4]+ [M+Na]+ [M+K]+ | 600.3164 605.2721 621.2459 | 582.2828 | C33H42O9 | 582.2829 | −0.13 | 81557-52-0 [116] 126372-45-0 [117] 126372-52-9 [117] 126372-50-7 [117] 515854-87-2 [118] 515854-85-0 [118] 515854-83-8 [118] 1253641-57-4 [119] 586971-22-4 [90,120] 1010414-43-3 [121] 944799-48-8 [122] | Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Jatrophane Lathyrane |
59 | 10.49 | [M+NH4]+ [M+Na]+ | 656.3428 661.2979 | 638.3087 | C36H46O10 | 638.3091 | −0.66 | / | / |
60 * | 10.54 | [M+NH4]+ [M+Na]+ [M+K]+ | 720.3378 725.2933 741.2679 | 702.3040 | C40H46O11 | 702.3040 | −0.06 | / | / |
61 | 11.33 | [M+NH4]+ [M+Na]+ [M+K]+ | 796.3330 801.2882 817.2620 | 778.2991 | C45H46O12 | 778.2989 | 0.22 | / | / |
62 | 11.41 | [M+H]+ [M+Na]+ [M+K]+ [2M+Na]+ | 551.2998 573.2823 589.2584 1123.5752 | 550.2931 | C33H42O7 | 550.2931 | 0.11 | 1010806-00-4 [122] 1811530-78-5 [123] 62820-23-9 [124] | Ingenane Ingenane Lathyrane |
63 * | 11.64 | [M+Na]+ [M+K]+ | 527.3705 543.3547 | 504.3819 | C31H52O5 | 504.3815 | 0.85 | / | / |
64 * | 12.51 | [M+NH4]+ [M+Na]+ | 812.3276 817.2827 | 794.2936 | C45H46O13 | 794.2938 | −0.27 | / | / |
65 | 12.81 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+NH4]+ [2M+Na]+ | 545.3471 562.3685 567.3292 583.3033 1106.7130 1111.6693 | 544.3400 | C32H48O7 | 544.3400 | -0.08 | 478243-87-7 [125] 92117-95-8 [113] 1020102-66-2 [126] 100217-91-2 [127] | Ingenane Tigliane Tigliane Tigliane |
66 | 12.96 | [M+H]+ [M+NH4]+ [M+Na]+ | 675.4103 692.4372 697.3922 | 674.4034 | C38H58O10 | 674.4030 | 0.61 | 76663-59-7 [30] 76663-58-6 [30] 76663-57-5 [30] 1362115-49-8 [128] | Ingenane Ingenane Ingenane Tigliane |
67 | 13.66 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+NH4]+ [2M+Na]+ | 623.3578 640.3875 645.3431 661.3140 1262.7365 1267.6896 | 622.3507 | C37H50O8 | 622.3506 | 0.20 | 149725-35-9 [129] | Daphnane |
68 * | 14.03 | [M+Na]+ [M+K]+ | 455.3518 471.3101 | 454.3453 | C30H46O3 | 454.3447 | 1.31 | 125456-55-5 [130,131] 125456-62-4 [130] 132831-05-1 [131] 94530-05-9 [132] 242814-44-4 [133] 1000000-03-2 [134] 1000000-04-3 [134] 2411214-36-1 [135] 2727156-37-6 [136] 2101307-34-8 [137] | Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene |
69 * | 14.22 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 651.4051 668.4155 673.3711 689.3442 | 650.3821 | C39H54O8 | 650.3819 | 0.30 | 184221-48-5 [138] 184221-44-1 [138] | Ingenane Ingenane |
70 * | 14.59 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 667.4206 684.4463 689.4023 705.3752 | 666.4132 | C40H58O8 | 666.4132 | −0.02 | / | / |
71 | 14.98 | [M+H]+ [M+Na]+ [M+K]+ | 617.4034 639.3868 655.3600 | 616.3975 | C36H56O8 | 616.3975 | −0.11 | 76663-56-4 [30] 1333380-60-1 [139] | Ingenane Ingenane |
72 | 15.63 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ [2M+NH4]+ [2M+Na]+ | 651.3885 668.4215 673.3714 689.3450 1318.8020 1323.7525 | 650.3826 | C39H54O8 | 650.3819 | 1.14 | 184221-48-5 [138] 184221-44-1 [138] | Ingenane Ingenane |
73 * | 15.84 | [M+H]+ [M+Na]+ [M+K]+ | 631.4203 653.4026 669.3765 | 630.4133 | C37H58O8 | 630.4132 | 0.23 | 57716-89-9 [140] 182997-47-3 [141] | Ingenane Tigliane |
74 * | 16.18 | [M+H]+ | 441.3727 | 440.3655 | C30H48O2 | 440.3654 | 0.06 | 142449-67-0 [131] 242814-43-3 [133] 242814-43-3 [133] 2067-65-4 [142] 110011-56-8 [34,142] 112406-53-8 [142] 122272-22-4 [143] 1650569-06-4 [144] 2413472-28-1 [145] 38242-02-3 [146] 6060-07-7 [146] 2852676-92-5 [146] 3866-77-1 [146,147] 543691-16-3 [148] 543691-17-4 [149] 543691-19-6 [149] 22478-71-3 [150] 1384465-02-4 [151] 138994-69-1 [152] 2004651-44-7 [153] 13159-28-9 [154] | Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene Triterpene |
75 | 17.25 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 653.5347 670.5604 675.5170 691.4930 | 652.5276 | C39H72O7 | 652.5278 | −0.34 | / | / |
76 | 17.28 | [M+Na]+ [M+K]+ | 623.3920 639.3661 | 600.4028 | C36H56O7 | 600.4026 | 0.33 | 1020102-70-8 [126] 349152-28-9 [155] | Tigliane Tigliane |
77 | 17.59 | [M+H]+ [M+Na]+ [M+K]+ [2M+NH4]+ [2M+Na]+ | 645.4368 667.4182 683.3931 1306.8935 1311.8487 | 644.4290 | C38H60O8 | 644.4288 | 0.27 | 76663-53-1 [30] 76663-55-3 [30] 76663-54-2 [30] 54706-69-3 [139,156] 2254317-50-3 [157] 20839-12-7 [128,158] 67492-54-0 [158] 73089-77-7 [158] | Ingenane Ingenane Ingenane Ingenane Ingenane Tigliane Tigliane Tigliane |
78 * | 17.78 | [M+H]+ [M+NH4]+ [M+Na]+ [M+K]+ | 371.3155 388.3419 393.2975 409.2714 | 370.3083 | C22H42O4 | 370.3083 | −0.08 | / | / |
79 * | 18.25 | [M+NH4]+ [M+Na]+ [M+K]+ | 652.4261 657.3764 673.3503 | 634.3871 | C39H54O7 | 634.3870 | 0.26 | / | / |
80 | 21.18 | [M+H]+ [M+Na]+ [M+K]+ | 629.4391 651.4233 667.3972 | 628.4340 | C38H60O7 | 628.4339 | 0.21 | 672945-80-1 [128,138] 1407160-19-3 [159] 1020102-72-0 [126] | Ingenane Ingenane Tigliane |
Extract | IC50, μg mL−1 | ||||
---|---|---|---|---|---|
NCI-H460 | NCI-H460/R | U87 | U87-TxR | MRC-5 | |
EC | 8.89 ± 2.55 | 33.48 ± 8.90 | 12.96 ± 4.14 | 12.22 ± 4.23 | 6.55 ± 2.64 |
ES | 20.11 ± 6.38 | 37.99 ± 18.72 | 15.71 ± 4.57 | 17.26 ± 4.10 | 5.89 ± 2.21 |
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Jadranin, M.; Savić, D.; Lupšić, E.; Podolski-Renić, A.; Pešić, M.; Tešević, V.; Milosavljević, S.; Krstić, G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. Plants 2023, 12, 4181. https://doi.org/10.3390/plants12244181
Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. Plants. 2023; 12(24):4181. https://doi.org/10.3390/plants12244181
Chicago/Turabian StyleJadranin, Milka, Danica Savić, Ema Lupšić, Ana Podolski-Renić, Milica Pešić, Vele Tešević, Slobodan Milosavljević, and Gordana Krstić. 2023. "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" Plants 12, no. 24: 4181. https://doi.org/10.3390/plants12244181