Next Article in Journal
The Effect of Wool Mulch on Plant Development in the Context of the Physical and Biological Conditions in Soil
Next Article in Special Issue
The Microbial Connection to Sustainable Agriculture
Previous Article in Journal
Effects of Co-Inoculating Saccharomyces spp. with Bradyrhizobium japonicum on Atmospheric Nitrogen Fixation in Soybeans (Glycine max (L.))
Previous Article in Special Issue
Changes in Microbial Community Structure in Response to Gummosis in Peach Tree Bark
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 12
Issue 3
10.3390/plants12030682
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops

by
Christian Drenker
1,
Doris El Mazouar
1,
Gerrit Bücker
1,2,
Sonja Weißhaupt
3,
Eveline Wienke
3,
Eckhard Koch
1,
Stefan Kunz
3,
Annette Reineke
2,
Yvonne Rondot
2 and
Ada Linkies
1,*
1
Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Biological Control, 69221 Dossenheim, Germany
2
Department of Crop Protection, Hochschule Geisenheim University, 65366 Geisenheim, Germany
3
Bio-Protect GmbH, 78467 Konstanz, Germany
*
Author to whom correspondence should be addressed.
Plants 2023, 12(3), 682; https://doi.org/10.3390/plants12030682
Submission received: 18 November 2022 / Revised: 23 January 2023 / Accepted: 30 January 2023 / Published: 3 February 2023
(This article belongs to the Special Issue Plant–Microbe Interactions for Sustainable Agriculture)
Browse Figures
Review Reports Versions Notes

Abstract

Although synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environment are urgently needed. In this context, we evaluated eight isolates of the genus Lysobacter (mainly Lysobacter enzymogenes) for their activity against plant pathogens. In vitro, the investigated Lysobacter isolates showed broad antagonistic activity against several phytopathogenic fungi, oomycetes and bacteria. Enzyme assays revealed diverse activities for the tested isolates. The most promising L. enzymogenes isolate (LEC) was used for further detailed analyses of its efficacy and effective working concentrations. The experiments included in vitro spore and sporangia germination tests and leaf disc assays as well as ad planta growth chamber trials against Alternaria solani and Phytophthora infestans on tomato plants, Pseudoperonospora cubensis on cucumbers and Venturia inaequalis on young potted apple trees. When applied on leaves, dilutions of a culture suspension of LEC had a concentration-dependent, protective effect against the tested pathogens. In all pathosystems tested, the effective concentrations were in the range of 2.5–5% and similarly efficacious to common plant protection agents containing copper hydroxide, wettable sulphur or fenhexamid. Thus, the isolate of L. enzymogenes identified in this study exhibits a broad activity against common plant pathogens and is therefore a promising candidate for the development of a microbial biocontrol agent.
Keywords: Lysobacter; biocontrol; antagonism; BCA; biological fungicide; plant protection Lysobacter; biocontrol; antagonism; BCA; biological fungicide; plant protection

Share and Cite

MDPI and ACS Style

Drenker, C.; El Mazouar, D.; Bücker, G.; Weißhaupt, S.; Wienke, E.; Koch, E.; Kunz, S.; Reineke, A.; Rondot, Y.; Linkies, A. Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops. Plants 2023, 12, 682. https://doi.org/10.3390/plants12030682

AMA Style

Drenker C, El Mazouar D, Bücker G, Weißhaupt S, Wienke E, Koch E, Kunz S, Reineke A, Rondot Y, Linkies A. Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops. Plants. 2023; 12(3):682. https://doi.org/10.3390/plants12030682

Chicago/Turabian Style

Drenker, Christian, Doris El Mazouar, Gerrit Bücker, Sonja Weißhaupt, Eveline Wienke, Eckhard Koch, Stefan Kunz, Annette Reineke, Yvonne Rondot, and Ada Linkies. 2023. "Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops" Plants 12, no. 3: 682. https://doi.org/10.3390/plants12030682

APA Style

Drenker, C., El Mazouar, D., Bücker, G., Weißhaupt, S., Wienke, E., Koch, E., Kunz, S., Reineke, A., Rondot, Y., & Linkies, A. (2023). Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops. Plants, 12(3), 682. https://doi.org/10.3390/plants12030682

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop