Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata (L.) Walp.) Landraces from Portugal and Mozambique
Abstract
:1. Introduction
2. Results
2.1. Overall Genetic Diversity
2.1.1. SSR Markers
2.1.2. SilicoDArT and SNP Markers
2.2. Diversity and Genetic Relationships between Accessions
2.2.1. SSR
2.2.2. SilicoDArT
2.2.3. SNP
2.3. Differentiation of Populations: Bayesian Approach and PCA Analysis
2.3.1. SSR
2.3.2. SilicoDArT
2.3.3. SNP
2.4. Comparison of Marker Systems
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. DNA Extraction, PCR Amplification and Fragment Sizing
4.3. Genotyping Cowpea Accessions Using SSR Markers
4.4. Genotyping Cowpea Accessions Using SilicoDArT and SNP Markers
4.5. Data Analysis
4.6. Quality Analysis of Marker Data
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Locus | Na | Ne | Ho | He | PIC |
---|---|---|---|---|---|
VM5 | 5 | 1.873 | 0.591 | 0.406 | 0.354 |
VM31 | 11 | 2.336 | 0.111 | 0.394 | 0.822 |
VM35 | 17 | 2.802 | 0.631 | 0.541 | 0.857 |
VM36 | 18 | 2.522 | 0.418 | 0.527 | 0.741 |
VM39 | 11 | 2.072 | 0.378 | 0.459 | 0.665 |
VuUGM05 | 26 | 3.439 | 0.199 | 0.462 | 0.834 |
VuUGM33 | 26 | 4.172 | 0.546 | 0.689 | 0.892 |
VuUGM71 | 27 | 4.588 | 0.682 | 0.710 | 0.851 |
CLM0634 | 4 | 1.781 | 0.053 | 0.282 | 0.403 |
CLM0721 | 16 | 1.975 | 0.100 | 0.424 | 0.618 |
CLM0767 | 7 | 1.807 | 0.003 | 0.290 | 0.401 |
CLM0792 | 15 | 1.947 | 0.187 | 0.348 | 0.645 |
Average | 15.25 | 2.61 | 0.33 | 0.46 | 0.67 |
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Guimarães, J.B.; Nunes, C.; Pereira, G.; Gomes, A.; Nhantumbo, N.; Cabrita, P.; Matos, J.; Simões, F.; Veloso, M.M. Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata (L.) Walp.) Landraces from Portugal and Mozambique. Plants 2023, 12, 846. https://doi.org/10.3390/plants12040846
Guimarães JB, Nunes C, Pereira G, Gomes A, Nhantumbo N, Cabrita P, Matos J, Simões F, Veloso MM. Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata (L.) Walp.) Landraces from Portugal and Mozambique. Plants. 2023; 12(4):846. https://doi.org/10.3390/plants12040846
Chicago/Turabian StyleGuimarães, Joana Bagoin, Cátia Nunes, Graça Pereira, Ana Gomes, Nascimento Nhantumbo, Paula Cabrita, José Matos, Fernanda Simões, and Maria Manuela Veloso. 2023. "Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata (L.) Walp.) Landraces from Portugal and Mozambique" Plants 12, no. 4: 846. https://doi.org/10.3390/plants12040846