Effects of Exogenous Auxin on Mesocotyl Elongation of Sorghum
Abstract
:1. Introduction
2. Results
2.1. The Effect of IAA Treatment to Mesocotyl Length of Different Sorghum Lines
2.2. The Elongation of Mesocotyl by IAA Treatment Depending on the Increase of Mesocotyl Cell Length
2.3. IAA Application Promote Energy Metabolism Process
2.4. IAA Application Promote Mesocotyl Length by Membrane Acidification
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Observation of Morphological Differences of Each Part of Mesocotyl
4.3. Starch and Soluble Sugar Content Measurement
4.4. Total Amylase Activity Measurement
4.5. Hexokinase and PM H+-ATPase Activity Measurement
4.6. RNA Extraction and Quantitative Real-Time PCR
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene | PCR Primer (5′-3′) |
---|---|
SbActin | ATGGCTGACGCCGAGGATATCCA GAGCCACACGGAGCTCGTTGTAG |
SbEXPB2 | GCGAGGTGAAGACGGTGATGATC GTGCCGGAGCTTGTCGTTGAG |
SbEXPB7 | CCTCCACCAGCCGTCGTCTAC CACCACCACCACTGCCGTTG |
SbEXPB8 | TAGGTAGTTGGATCGGAGCAGAGC CAGGCAGGAGAGCGAGGACAG |
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Liu, C.; Yao, Z.; Jiang, B.; Yu, W.; Wang, Y.; Dong, W.; Li, Y.; Shi, X.; Liu, C.; Zhou, Y. Effects of Exogenous Auxin on Mesocotyl Elongation of Sorghum. Plants 2023, 12, 944. https://doi.org/10.3390/plants12040944
Liu C, Yao Z, Jiang B, Yu W, Wang Y, Dong W, Li Y, Shi X, Liu C, Zhou Y. Effects of Exogenous Auxin on Mesocotyl Elongation of Sorghum. Plants. 2023; 12(4):944. https://doi.org/10.3390/plants12040944
Chicago/Turabian StyleLiu, Chang, Ziqing Yao, Bing Jiang, Wenbo Yu, Yu Wang, Wenhui Dong, Yutong Li, Xiaolong Shi, Chunjuan Liu, and Yufei Zhou. 2023. "Effects of Exogenous Auxin on Mesocotyl Elongation of Sorghum" Plants 12, no. 4: 944. https://doi.org/10.3390/plants12040944