Proteome Analysis of Nicotiana tabacum Cells following Isonitrosoacetophenone Treatment Reveals Defence-Related Responses Associated with Priming
Abstract
:1. Introduction
2. Results
2.1. Two-Dimensional Electrophoresis
2.2. iTRAQ LC-MS Analysis
3. Discussion
3.1. Adaption-Related Proteins Responsive to INAP Treatment
3.1.1. Biosynthesis and Redirection of Resources
3.1.2. Defence
3.1.3. DNA and Transcription
3.1.4. Growth, Metabolism and Energy
3.1.5. Stress-Related Responses and Signalling Events
3.1.6. Translation
3.1.7. Transport
3.2. Basic Functionality Proteins
Mitochondrial Activity
4. Materials and Methods
4.1. Cell Suspension Cultures, INAP Treatment and Experimental Design
4.2. Protein Extraction and Two-Dimensional Gel Electrophoresis
4.3. Protein Extraction for iTRAQ
4.4. Sample Preparation and Labelling for 8-plex iTRAQ
4.5. OFFGEL Electrophoresis
4.6. LC MS/MS Analysis
4.7. Data Analysis and Database Searching
4.8. Data Analysis: Protein Identification Criteria and Bioinformatics Validation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein Name | Accession Number | 0 h (T) | 8 h (T) | 16 h (T) | 24 h (T) | Gene Accession Number | Gene Name |
---|---|---|---|---|---|---|---|
Biosynthesis | |||||||
UDP-glucose:protein transglucosylase-like protein SlUPTG1 1 | Q6IV07_SOLLC | 0.0 | 0.2 | −0.3 | 0.0 | XM 016639837.1 a | Alpha-1,4-glucan-protein synthase |
Uncharacterised protein 2 | M1C547_SOLTU | 0.0 | 0.7 | 0.3 | 0.5 | XM 016633077.1 a | Isopentenyl-diphosphate delta-isomerase I |
Uncharacterised protein 2 | M1C5Y4_SOLTU | 0.0 | 1.2 | −0.3 | 0.4 | NM 001326080.1 | UDP-glucuronic acid decarboxylase 6-like |
Uncharacterised protein 1 | K4BVZ4_SOLLC | 0.0 | −0.2 | −0.6 | −0.3 | XM 016647169.1 b | Dolichyl-diphosphooligosaccharide-protein glycosyltrasnferase |
Uncharacterised protein 1 | K4CET6_SOLLC (+1) | 0.0 | 0.2 | −0.8 | −0.3 | XM 016657513.1 b | Long chain acyl-CoA synthetase 8-like |
Cystathionine gamma-synthase 3 | Q9ZPL5_TOBAC | 0.0 | 0.0 | −0.6 | −0.4 | AF097180.1 | Alpha-1,4-glucan-protein synthase |
Defence | |||||||
Annexin 3 | Q9XEN8_TOBAC | 0.0 | 0.6 | 0.4 | 0.4 | NM 001325957.1 | |
Putative hydroxycinnamoyl transferase 5 | B5LAV0_CAPAN (+5) | 0.0 | 0.5 | −0.1 | 0.3 | NM 001325623.1 | Shikimate O-hydroxy-cinnamoyltransferase |
Superoxide dismutase (Cu-Zn) 8 | SODC_NICPL | 0.0 | −0.4 | −0.8 | −0.4 | XM 016594235.1 a | |
Polygalacturonase inhibiting protein 4 | C4PG28_9SOLN | 0.0 | −0.3 | −0.6 | −0.3 | XM 016650938.1 a | Polygalacturonase inhibitor-like |
Uncharacterised protein 1 | K4ASK1_SOLLC | 0.0 | 0.1 | −0.9 | −0.3 | XM 016578137.1 a | Callose synthase 9-like |
DNA and Transcription | |||||||
Uncharacterised protein 1 | K4CMV1_SOLLC | 0.0 | −0.6 | −0.7 | −0.9 | XR 001654730.1 b | Replication protein A 70kDa DNA-binding |
XM 016640989.1 b | |||||||
Endonuclease 1 1 | G3XKQ7_SOLLC (+2) | 0.0 | −0.2 | −0.6 | −0.5 | XM 016636178.1 a | |
Histone H2B 6 | A2IBL2_NICBE | 0.0 | 0.1 | −0.6 | −0.2 | ||
Histone H2B 3 | H2B_TOBAC | 0.0 | −0.4 | −0.7 | −0.2 | ||
Growth | |||||||
Uncharacterised protein 1 | K4BLX1_SOLLC | 0.0 | 0.0 | −0.7 | −0.4 | XM 016647000.1 b | BONZAI 1-like |
XM 016649340.1 b | |||||||
DWARF1/DIMINUTO 1 | Q66YT8_SOLLC | 0.0 | −0.2 | −0.7 | −0.4 | XM 016612143.1 a | Delta(24)-sterol reductase-like |
Uncharacterised protein 1 | K4BGV0_SOLLC | 0.0 | 0.1 | 0.7 | 0.0 | XM 016614569.1 b | CDK5RAP3-like protein |
Metabolism and Energy | |||||||
Uncharacterised protein 1 | K4BDG0_SOLLC (+2) | 0.0 | −0.1 | −1.3 | −0.7 | XM 016594393.1 b | Very-long-chain 3-oxoacyl-CoA reductase 1-like |
XM 016626206.1 b | |||||||
Uncharacterised protein 2 | M1A251_SOLTU | 0.0 | −0.4 | −0.8 | −0.5 | XM 016617189.1 b | Suberization-associated anionic peroxidase 1-like |
Probable pectate lyase P18 1 | K4BDF4_SOLLC | 0.0 | 0.1 | −0.2 | 0.0 | XM 016621815.1 b | |
Uncharacterised protein 1 | K4AT35_SOLLC (+2) | 0.0 | −0.2 | 0.0 | 0.6 | XM 0166417471 a | 26S Proteasome non-ATPase |
XM 016629739.1 a | |||||||
Glycylpeptide N-tetradecanoyltransferase 2 | M0ZRQ4_SOLTU | 0.0 | 0.5 | 0.1 | 0.4 | XM 016628510.1 a | |
Methylenetetrahydro-folate reductase 1 | K4C2P8_SOLLC (+2) | 0.0 | 0.7 | 0.4 | 0.5 | XM 016660349.1 a | |
XM 016660348.1 a XM 016660347.1 a | |||||||
Response | |||||||
Glycine-rich RNA-binding protein 3 | B2YKT9_TOBAC | 0.0 | 0.1 | 0.4 | 0.6 | ||
Putative methyltransferase 2 | A4UV20_SOLTU (+3) | 0.0 | −0.3 | −0.8 | −0.4 | NM 001324795.1 | |
LC052785.1 | |||||||
Uncharacterised protein 2 | M1C4X5_SOLTU | 0.0 | −0.3 | −0.9 | −0.9 | XM 016650151.1 b XM 016650150.1 b | Probable methyltransferase |
Signalling | |||||||
Ras-related GTP-binding protein 3 | Q40569_TOBAC | 0.0 | 0.1 | −0.7 | −0.4 | NM 001325922.1 X72212.1 | |
Uncharacterised protein 2 | M1B9Y5_SOLTU | 0.0 | 0.0 | −0.7 | −0.3 | XM 016620909.1 b | Putative receptor kinase |
Calcium-dependent protein kinase 3 | O81390_TOBAC | 0.0 | −0.5 | −0.7 | −0.5 | NM 001324640.1 AF072908.1 | |
Beta-tubulin 9 | Q676U1_NICAT | 0.0 | 0.6 | 0.2 | 0.2 | NM 001325510.1 | |
KP316400.1 | |||||||
Alpha subunit of SnRK1 10 | M1LH79_9SOLN | 0.0 | 0.8 | 0.1 | 0.2 | XM 016633060.1 a | |
Phospholipase D 1 | K4BAK2_SOLLC | 0.0 | 0.6 | −1.1 | −0.6 | XM 016601192.1 b | Ras-related protein RAB8-1 |
Translation | |||||||
Peptidyl-prolyl cis-trans isomerase 5 | B1PDK0_CAPAN | 0.0 | 0.4 | 0.6 | 0.2 | XM 016614927.1 b | |
Transport | |||||||
Clathrin heavy chain 1 | K4C1T2_SOLLC | 0.0 | −0.2 | −0.6 | −0.5 | XM 016641028.1 a | Clathrin heavy chain 1 |
Plasma membrane ATPase 4 8 | PMA4_NICPL | 0.0 | 0.0 | −0.7 | −0.4 | XM 016619330.1 a | |
Uncharacterised protein 2 | M1D6E0_SOLTU | 0.0 | −0.1 | −0.7 | −0.4 | XM 016602228.1 a | ATPase 2 |
Pleiotropic drug resistance protein 1 8 | PDR1_NICPL | 0.0 | −0.2 | −1.1 | −0.5 | XM 016622655.1 a | |
Clathrin heavy chain 1 | K4C5S4_SOLLC | 0.0 | −0.3 | −0.8 | −0.8 | XM 016613710.1 a | |
Reticulon-like protein 2 | M0ZGB8_SOLTU | 0.0 | 0.1 | −0.8 | 0.0 | XM 016611959.1 b | |
Uncharacterised protein 1 | K4CIM3_SOLLC (+2) | 0.0 | −0.4 | −0.9 | −1.0 | XM 016622951.1 b | Protein transport protein SEC16B |
Basic Functionality Proteins | |||||||
Glycine dehydrogenase (decarboxylating) 2 | GCSP_SOLTU (+2) | 0.0 | 0.4 | 0.5 | 0.5 | XM 016601634.1 a | |
Aspartate aminotransferase 1 | K4CG60_SOLLC | 0.0 | 0.5 | 0.5 | 0.6 | XM 016642299.1 a | |
Uncharacterised protein 1 | K4C412_SOLLC | 0.0 | −0.2 | −0.4 | −0.6 | XM 016617152.1 a | Fumarate hydratase 1 |
Tobacco pre-pro-cysteine proteinase3 | Q43579_TOBAC (+2) | 0.0 | 0.1 | 0.0 | 0.3 | Z13959.1 | |
Cytochrome c oxidase subunit 2 3 | Q5MA02_TOBAC | 0.0 | 0.1 | −0.7 | −0.5 | ||
Mitochondrial small heat shock protein 5 | D9IAX1_CAPAN (+1) | 0.0 | −0.1 | −0.6 | 0.0 | ||
Uncharacterised protein 2 | M0ZUV5_SOLTU | 0.0 | −0.2 | −0.8 | −0.4 | XM 016634781.1 b | Cytochrome P450 |
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da Camara, N.; Dubery, I.A.; Piater, L.A. Proteome Analysis of Nicotiana tabacum Cells following Isonitrosoacetophenone Treatment Reveals Defence-Related Responses Associated with Priming. Plants 2023, 12, 1137. https://doi.org/10.3390/plants12051137
da Camara N, Dubery IA, Piater LA. Proteome Analysis of Nicotiana tabacum Cells following Isonitrosoacetophenone Treatment Reveals Defence-Related Responses Associated with Priming. Plants. 2023; 12(5):1137. https://doi.org/10.3390/plants12051137
Chicago/Turabian Styleda Camara, Nikita, Ian A. Dubery, and Lizelle A. Piater. 2023. "Proteome Analysis of Nicotiana tabacum Cells following Isonitrosoacetophenone Treatment Reveals Defence-Related Responses Associated with Priming" Plants 12, no. 5: 1137. https://doi.org/10.3390/plants12051137