Biomass and Leaf Nutrition Contents of Selected Grass and Legume Species in High Altitude Rangelands of Kashmir Himalaya Valley (Jammu & Kashmir), India
by
, , , and
Javed A. Mugloo
1,
Mehraj ud din Khanday
2,
Mehraj ud din Dar
1,
Ishrat Saleem
1,
Hesham F. Alharby
3,4,
Atif A. Bamagoos
3,
Sameera A. Alghamdi
3,
Awatif M. Abdulmajeed
5,
Pankaj Kumar
6
and
Sami Abou Fayssal
7,8,*
1
Division of Silviculture and Agro Forestry, Faculty of Forestry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir 190025, India
2
Division of Soil Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir 190025, India
3
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
4
Plant Biology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
5
Biology Department, Faculty of Science, University of Tabuk, Umluj 46429, Saudi Arabia
6
Agro-Ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri (Deemed to Be University), Haridwar 249404, India
7
Department of Agronomy, Faculty of Agronomy, University of Forestry, 10 Kliment Ohridski Blvd, 1797 Sofia, Bulgaria
8
Department of Plant Production, Faculty of Agriculture, Lebanese University, Beirut 1302, Lebanon
*
Author to whom correspondence should be addressed.
Plants 2023, 12(7), 1448; https://doi.org/10.3390/plants12071448
Submission received: 12 November 2022
/
Revised: 10 February 2023
/
Accepted: 20 March 2023
/
Published: 25 March 2023
(This article belongs to the Special Issue Ecophysiology and Ecology of Grassland)
Abstract
:The yield and nutritional profile of grass and legume species in Kashmir Valley’s rangelands are scantly reported. The study area in this paper included three types of sites (grazed, protected, and seed-sown) divided into three circles: northern, central, and southern Kashmir. From each circle, three districts and three villages per district were selected. Most sites showed higher aboveground biomass (AGB) compared to belowground biomass (BGB), which showed low to moderate effects on biomass. The comparison between northern, central, and southern Kashmir regions revealed that AGB (86.74, 78.62, and 75.22 t. ha−1), BGB (52.04, 51.16, and 50.99 t. ha−1), and total biomass yield (138.78, 129.78, and 126.21 t. ha−1) were the highest in central Kashmir region, followed by southern and northern Kashmir regions, respectively. More precisely, AGB and total biomass yield recorded the highest values in the protected sites of the central Kashmir region, whereas BGB scored the highest value in the protected sites of southern Kashmir region. The maximum yield (12.5 t. ha−1) recorded among prominent grasses was attributed to orchard grass, while the highest crude fiber and crude protein contents (34.2% and 10.4%, respectively), were observed for Agrostis grass. The maximum yield and crude fiber content (25.4 t. ha−1 and 22.7%, respectively), among prominent legumes were recorded for red clover. The highest crude protein content (33.2%) was attributed to white clover. Those findings concluded the successful management of Kashmir rangelands in protected sites, resulting in high biomass yields along with the considerable nutritional value of grasses and legumes.
1. Introduction
The Jammu and Kashmir (J and K) region (India) is well known for its alpine and subalpine pastures [1], locally named “Margs” or “Bahaks”. These pastures constitute a crucial ecological resource and play a significant role in the socioeconomic state of the Himalayas Valley. The total area of Kashmir pasturelands is around 9595 km2 [2]. These pasturelands are rural areas that involve around 97% of the population in the agricultural sector. The rearing of sheep, goats, and cattle constitutes the locals’ subsidiary occupation. In addition, a huge population of nomadic Bakerwals, Gujjars, Chopans, Changpas, and Gaddies depends directly on meadow products and pasturelands for herds of maintained livestock.
The term “grassland” (also designated by “pastureland”) can be defined as land (and the vegetation growing on it) devoted to the production of introduced or indigenous forage for harvest via grazing, cutting, or both. The grassland’s vegetation includes grasses, legumes, and scantly woody species [3]. Thus, grassland is a highly dynamic ecosystem that supports fauna, flora, and human populations worldwide. It also encloses fodder crops that covered approximately 3.5 billion hectares in 2000 and contains around 20% of the world’s soil carbon stocks [4]. These stocks can be well enriched through the good management of grasslands via Voisin’s rational grazing (VRG), resulting in an increased milk production by ruminants [5]. This system allows the maximization of pasture growth associated with ruminant intake while maintaining a sustainable circular cycle [6]. The adoption of such a system is a must, especially in the Himalayan pasturelands, which was a scene of overgrazing over decades and centuries [7]. The literature reported a decrease in the available grazing area in the alpine and subalpine pasturelands of Kashmir from 0.15 ha. animal−1 to 0.10 ha. animal−1 between 1977 and 1982 [8]. Unfortunately, it is still at a continuous decrease rate according to a recent report [9].
As the globe is still facing a rise in climatic change, there is an increased demand to monitor, forecast and predict its effect on the productivity and quality of pasturelands [10]. However, the prediction of pasturelands’ biomass is sometimes not very reliable, while the accurate and precise estimation consists of traditional methods that are mainly costly, time-consuming, and non-environmentally friendly. In addition to this, the traditional influencing factors (i.e., high wind velocity, low temperature, snowstorms, and so on) and the increase in solar and ultraviolet (UV) radiations—as a direct response to climate change—have resulted in the decreased biomass production of Himalaya pasturelands [11]. Similar observations were acknowledged in Nepal [12] and in the African pasturelands of Niger and Zambia [10,13]. Thus, since the crucial role of pastureland in ecosystem functioning and soil stability is endangered, the safeguarding of the Himalayan alpine and subalpine vegetation exerts its weight [14].
Although earlier studies focused on the aboveground herbaceous species production in a very limited area in Kashmir Valley [15], none have explored the whole region’s above and belowground biomass (AGB and BGB, respectively), nor documented reliably the prominent grasses and legumes species growing there. These grasses and legumes are the main diet for sheep, goats, and cattle reared in the region. Thus, as the local population mainly relies on milk production, the compositional quality evaluation of biomass used for sheep, goats, and cattle nutrition is crucial. Therefore, the current study aimed to (a) investigate and estimate the biomass yield and leaf nutritional profile of grasses and legumes in high-altitude pasturelands of the northern, central and southern Kashmir Himalaya Valley (Jammu and Kashmir (J&K)), India; and (b) detect any possible effect (danger i.e., overgrazing) on grassland biomass in the studied zones.
2. Results
2.1. Analysis of Above (AGB), Below Ground Biomass (BGB) and Total Biomass Yield in Northern Kashmir
In northern Kashmir region, the average AGB/BGB ratio was the highest in the grazed and seed-sown sites of Kupwara district (1.61 and 1.85, respectively), and in the protected sites of Baramulla district (1.38) (Table 1). The average ratio of protected sites biomass over grazed sites biomass (R1) was comparable between districts, whereas the average ratio of seed-sown sites’ biomass over grazed sites’ biomass (R2) was the highest in Kupwara district. The percentages of villages within districts, where AGB > BGB (P1), and seed-sown sites biomass > grazed sites biomass (P3), were the highest in Kupwara district (100%). All districts showed a comparable percentage (100%) of villages where protected sites biomass > grazed sites biomass (P2).
All northern Kashmir districts showed higher AGB by 32.0%–43.2% in grazed sites compared to BGB, except in Firozpur (Baramulla district) and Ketsan (Bandipora district) where BGB was higher (p < 0.05) by 40.0% than AGB (Table 2). In protected sites, AGB was also higher (p < 0.05) by a range of 1.4%–40.3% than BGB, except in Ketsan (Bandipora district) where the latter was higher (p < 0.05) by 12.3% than the former. In all northern Kashmir districts, seed-grown sites showed higher (p < 0.05) AGB by a range of 20.1%–56.4% than BGB, whereas BGB was more abundant than AGB (p < 0.05) by 15.3% and 12.1% in Firozpur (Baramulla district) and Ketsan (Bandipora district), respectively. It was also depicted that the average biomass was higher (p < 0.05) for AGB than BGB in all districts (24.4%–43.2%), except for Firozpur (Baramulla district) and Ketsan (Bandipora district) (14.8% and 18.6%, respectively). Rajwar (Kupwara district), Gulmarg (Baramulla district), and Cithernaar (Bandipora district) showed the highest AGB and BGB among all districts and studied sites of northern Kashmir region. On the other hand, Gulmarg (Baramulla district) had the highest (p < 0.05) AGB among all studied sites, while Rajwar (Kupwara district) and Cithernaar (Bandipora district) had the highest (p < 0.05) BGB.
2.2. Analysis of Above (AGB), Below Ground Biomass (BGB) and Total Biomass Yield in Central Kashmir
In central Kashmir region, the average AGB/BGB ratio was the highest in all studied sites of Budgam district (2.08, 1.72, and 2.11 at grazed, protected and seed-sown sites, respectively), (Table 3). R1 was comparable between Ganderbal and Srinagar districts (2.70), whereas R2 was the highest in Srinagar district (1.53). P1, P2, and P3 were comparable between all central Kashmir districts (100%).
All central Kashmir districts showed a higher AGB (p < 0.05) by 19.7%–70.2% in grazed sites than BGB (Table 4). In protected sites, AGB was also higher (p < 0.05) by a range of 12.8%–47.9% than BGB. In all central Kashmir districts, seed-grown sites showed higher (p < 0.05) AGB by a range of 18.7%–57.1% than BGB. Moreover, the average biomass was higher (p < 0.05) for AGB than BGB in all districts (20.8%–55.6%). Narang (Ganderbal district), Kanidajan (Budgam district), and Chirenbal (Srinagar district) showed the highest AGB and BGB among all districts and studied sites of central Kashmir region. On the other hand, Kanidajan (Budgam district) showed significantly higher (p < 0.05) AGB and BGB in all sites except protected ones.
2.3. Analysis of Above (AGB), Below Ground Biomass (BGB) and Total Biomass Yield in Southern Kashmir
In southern Kashmir region, the average AGB/BGB ratio was the highest in the grazed and seed-sown sites of Anantnag district (1.71 and 1.82, respectively), and the protected sites of Shopian district (1.38) (Table 5). R1 and R2, and P1 and P3 scored the highest values in Anantnag and Kulgam districts, respectively, (2.38 and 1.47, and 100%, respectively). P3 was comparable between all southern Kashmir districts (100%).
Particularly, all southern Kashmir districts showed a higher AGB (p < 005) by 31.6%–51.9% in grazed sites than BGB, except in Dabjan (Shopian district) and Astanmarg (Kulgam district) where BGB was higher (p < 0.05) by 30.6% and 42.0% than AGB, respectively (Table 6). The same trend was observed regarding protected sites where AGB were higher (p < 0.05) by a range of 2.0%–40.3% than BGB, except in Dabjan (Shopian district) and Astanmarg (Kulgam district) where BGB was higher (p < 0.05) by 5.4% and 18.0% than AGB, respectively. In all southern Kashmir districts, seed-grown sites showed higher (p < 0.05) AGB by a range of 17.4%–56.6% than BGB, whereas BGB was more abundant (p < 0.05) by 15.3% and 12.8% than AGB in Dabjan (Shopian district) and Astanmarg (Kulgam district), respectively. Also, the average biomass was higher (p < 0.05) for AGB over BGB in all districts (14.9%–44.3%), except for Dabjan (Shopian district) and Astanmarg (Kulgam district) (15.1% and 22.0%, respectively). Aru Valley (Anantnag district), Kaller (Shopian district), and Chirenbal (Kulgam district) showed the highest AGB and BGB among all districts and studied sites. On the other hand, AGB was the highest (p < 0.05) in Kaller (Shopian district) in grazed and protected sites, and in Chirenbal (Kulgam district) among seed-sown sites. BGB was the highest (p < 0.05) in Chirenbal (Kulgam district) in grazed and seed-grown sites, and in Aru Valley (Anantnag district) in protected sites.
2.4. Comparison of Above (AGB), Belowground Biomass (BGB) and Total Biomass Yield between Kashmir Regions
The comparison between northern, central, and southern Kashmir regions revealed that AGB (86.74, 78.62, and 75.22 t. ha−1), BGB (52.04, 51.16, and 50.99 t. ha−1), and total biomass yield (138.78, 129.78, and 126.21 t. ha−1) were the highest in central Kashmir region, followed by southern and northern Kashmir ones, respectively, (Figure 1). More precisely, AGB and total biomass yield recorded the highest values in the protected sites of central Kashmir region (42.04 and 68.41 t. ha−1, respectively), whereas BGB scored the highest value in the protected sites of southern Kashmir region (27.00 t. ha−1).
2.5. Yield and Nutrient Profile of Prominent Grasses and Legumes
The results of prominent grasses’ yield and nutrient profile evaluation are shown in Table 7. Orchard grass (Dactylis glomerata) showed the highest yield (p < 0.05) (12.50 ± 0.6 t. ha−1) compared to other grasses, whereas, the highest nitrogen content (p < 0.05) was detected in Timothy grass (1.81 ± 0.05%). Phosphorus and crude protein contents were the most abundant (p < 0.05) in Agrostis grass (Agrostis alba) (0.34 ± 0.04% and 10.40 ± 0.5%, respectively), whereas perennial ryegrass (Lolium perenne) enclosed the highest (p < 0.05) potassium and crude fiber contents (0.44 ± 0.05% and 35.12 ± 0.8%, respectively). It should be noted that no significant difference (p > 0.05) and very low standard deviations (SDs) (0.2 < SD < 0.6, and 0.01 < SD < 0.8) were observed in terms of grass yield, and leaf nutrient content, respectively, between all studied sites in the three Kashmir regions.
Table 8 showed that red clover (Trifolium pretense) had the highest (p < 0.05) yield, nitrogen, potassium, and crude protein contents (25.40 ± 0.6 t. ha−1, 1.69 ± 0.05%, 0.45 ± 0.05%, and 22.70 ± 0.6%, respectively), among prominent legumes found in Kashmir Valley, whereas the phosphorus content was the most abundantly found (p < 0.05) in white clover (Trifolium repens) and sainfoin (Onobrychis viciifolia) (0.34 ± 0.04%). In addition, the crude fiber content scored its highest value in white clover (33.12 ± 0.8%) compared to other prominent legumes studied. It should be noted that no significant difference (p > 0.05) and very low SDs (0.3 < SD < 0.6, and 0.02 < SD < 0.8) were observed in terms of legume yield and leaf nutrient content, respectively, between all studied sites in the three Kashmir regions.
3. Discussion
3.1. Analysis of Above (AGB), Belowground Biomass (BGB), and Total Biomass Yield
The healthy functioning of an ecosystem can be estimated by the overall plant biomass it yields. Over-grazing is one of the most critical issues facing plant biomass in Kashmiri grasslands. The current study detected higher AGB compared to BGB and a positive AGB/BGB ratio in all districts of northern, central, and southern Kashmir regions. This simulates that the studied locations were not over-grazed. However, the high BGB in Aru Valley, Kaller and Chirenbal (northern Kashmir), Rajwar, Gulmarg, and Cithernaar (southern Kashmir), and Naranag, Kanidajan, and Chirenbal (central Kashmir) might reveal possible moderate grazing within these regions. Such regions might be abandoned after being heavily grazed in elder decades. In this regard, Dai et al. [16] reported that the moderate grazing promoted the root biomass of Kobresia meadow (BGB) in the northern Qinghai-Tibet pastures, which corroborates with our findings. Thus, the adaptive response of plants might occur in which they tend to increase their root development to survive [17]. Also, during the April-May period, snow melting occurs in the pasturelands of Kashmir Valley which naturally favors and promotes the growth and development of BGB. Furthermore, the protected areas (sites) by local authorities may have helped in the preservation of plant biomass away from rearing and over-grazing, thus resulting in increased biomass yields. This is consistent with the report of Lone and Pandit [18] on the Langate Forest division of Kashmir. Tittonell et al. [19] proposed an agroecological research agenda, suggesting species breeding to preserve diversity and a co-innovation of large-scale farming with farmers, policymakers, and value chains. Although site protection resulted in substantial improvements in rangeland grazing management in Namibia, it did not enhance cattle productivity nor rangeland health [20]. The interesting observation in the present study is that all studied species were found in grazed, protected, and seed-sown sites, which outlines again that grasslands were not over-grazed in the studied locations. Two decades back, it was reported that dry matter biomass yield ranged between 1.41 and 6.23 t. ha−1 in temperate pastures of the northwestern Himalayas [21], which is far below that observed in the current study. This could be explained by the fact that Kashmiri citizens are now more conscious and aware of the risks on grassland biomass and the disequilibrium of the environmental balance associated with overgrazing. On the other hand, climatic contrasts, species heterogeneity, and anthropogenic disturbances were reported to affect the biomass yield in the lesser Himalayan foothills, and northwestern regions of Kashmir Valley [22,23]. This simulates a possible inclusion of non-native species to the studied sites, resulting in the variation of soil organic carbon (SOC), and thus a variation in biomass yields [24]. Our findings outlined that AGB, BGB, and total biomass yield were the highest in central Kashmir, followed by southern and northern Kashmir. Such a variation in biomass yields was reported to be correlated with the variation in carbon sequestration potential (CSP) [25]. Panwar et al. [25] reported a high CSP in northern India (J & K as a whole state), associated with high biomass yields (AGB: 6.7–159.4 t. ha−1; BGB: 1.6–71.5 t. ha−1; total biomass yield: 15.9–202.6 t. ha−1). However, the study was very general and did not take into consideration the difference between Kashmiri regions in terms of vegetative populations nor site types (grazed, protected and seed-sown sites). Despite that, the AGB, BGB, and total biomass yields observed in the present study fall within the ranges stated in the aforementioned ones.
3.2. Yield and Nutrient Profile of Prominent Grasses and Legumes
The nutritional composition of grasses used for animal forage is a factor determining their growth, reproduction, and livestock production. Additionally, the climate, soil type, and degree of maturity of grasses influence their nutritional composition [26,27]. Sampling was performed during July which means that the studied grasses and legumes were at their harvest stage [28]. This simulates that their nutrient richness may have started to decline [29]. On the other hand, Hao and He [30] outlined an increased biomass yield once a nutrient loss occurs. This statement partly agrees with our findings as biomass yield was satisfying while grasses were highly nutritious. Leaf N, P, and K contents in orchard grass was several folds higher than outlined in other grasslands (N: 0.22–0.26%, p: 0.002–0.004%, K: 0.007–0.02%) [31]. A previous study on Chinese seed-sown pastures outlined leaf P and K contents in tall fescue grass higher by 1.7-fold and 4.9-fold than our findings [32]. Generally, leaf nitrogen content in grasses should not exceed 3.5% [33]; thus, our findings showed safe values. Leaf phosphorus content in grasses does not usually exceed 0.7–0.8% [34], which agrees with our findings, whereas leaf potassium content can range between 1.2 and 2.0% [35]. This simulates that the selected grasses may show some K deficiency. Moreover, Chang et al. [32] depicted a crude protein content in the range of 11–14%, being 1.5–1.9-fold higher than observed in the present study. On the other hand, the crude protein and crude fiber contents in selected legumes was promising. It was recommended that 12–19% of crude protein would be suitable for cattle feed [36]; which means that some of the selected grasses can be mixed with red or white clover to improve the protein requirements for rearing cattle. Thus, the high management of grasslands is proposed to increase the crude protein and crude fiber contents in selected grasses. In this context, Berauer et al. [37] reported that crude protein in grasses increased by 22–30% after high land management. Furthermore, the moderate nitrogen percentages in the studied grasses revealed the absence of an over-grazing activity in the studied sites. Dong et al. [38] explained that increased N rates, N mineralization, and nitrification processes occur when associated with over-grazing. It is worth noting that animals have different behaviors based on their preference. For instance, cattle and sheep diets are mainly based on grasses and legumes while goats’ diet is more related to herbal biomass [39]. Therefore, the present grasslands studied enclose nutritious grasses and legumes highly abundant for cattle and sheep foraging. This cannot be achieved without the inhibition of biomass species’ eradication unless correct and serious management of lands is performed associated with new technology that is timesaving and has a lower negative impact on the environment.
4. Materials and Methods
4.1. Study Area and Sites Description
The study area enclosed the whole Jammu and Kashmir (J and K) Valley, which was divided into three zone circles: northern, central, and southern. Within each circle, three districts were selected, and subsequently, three sites within each district were chosen (Table 9). All studied sites varied in elevation between 1450 and 4800 m above mean sea level. Studied areas enclosed: (a) Grazed Sites, (b) Protected Sites, and (c) Seed-Sown Sites. Grazed sites are grassland areas covered by grasses and legumes that are suitable for livestock grazing. Protected sites are grassland areas where carbon emissions from land use change are limited, and nutrient sapping is avoided. This included the cultivation of trees as windbreaks to reduce soil erosion and crop rotation to keep good nutrient availability in soil. Seed-sown sites are grassland areas which were seeded with native engendered species due to overgrazing or extensive agricultural exploitation. Seeded species included: orchard, tall fescue, perennial rye, and Agrostis grasses, and legumes such as: red clover, white clover, lucerne, sainfoin, and crown vetch. These species were sown in a randomized complete block design (RCBD) between mid-August and mid-September at a rate of 36 g seeds/m2. A harrowing process was also practiced for initial soil preparation in order to optimize seedling establishment.
4.2. Climate
The union territory of J and K, India (33°17′–37°20′ N latitude, 73°25′–80°30′ E longitude) comprises two main physical regions: Outer Himalayas with sub-tropical and intermediate climate (Jammu), and Inner Himalayas with a temperate climate (Kashmir). The climate varies considerably with altitude; it is mild and salubrious in lower altitudes but very cold in higher-ups. Spring is cool and rather wet. Regarding the Outer Himalayas (Jammu), average minimum and maximum temperatures vary between −11 °C and 33 °C during winter and summer, respectively. Autumn is bright and pleasant, while winter is extremely cold and experiences heavy snowfalls. Frost is experienced from the middle of November onwards. The main form of precipitation is snow in winter and some stray rains, and showering in spring. The Jammu region receives an average annual precipitation of about 1103 mm in the form of rain and snow for about 70 days. Unlike the Outer Himalayas, there is no distinct rainy season in the Inner Himalayas. The minimum temperature of the Kashmir region falls within −7 °C in winter and the maximum goes up to 35 °C in summer. It is characterized by a mean minimum temperature below 8 °C for more than six consecutive months per year. Mean annual minimum and maximum temperatures range between 6.68 and 19.31 °C, respectively, and mean annual soil temperature ranges between 8 and 15 °C, thus the area belongs to the mesic temperature regime. The mean annual rainfall in the Inner Himalayas is 710 mm and the soil in the studied area does not remain generally dry for more than 90 cumulative days. Hence, it belongs to the udic moisture regime.
4.3. Vegetation Diversity
Several types of grasses and legumes growing in the studied Kashmir regions are outlined in Table 10.
4.4. Sampling
Samples were collected following a direct field plot harvest method [40]. Briefly, three transects, divided into two blocks (100 m distant from each other) were performed. Moreover, three quadrants of 1 m2 each were performed within each block. All grasses and legumes, within these quadrants, were collected from their roots by digging 5 cm2 pits up to a depth of 30 cm. Then, they were packed in ice-cooled-bags, transported directly to the laboratory for identification, and stored at a cool temperature for further analyses.
4.5. Compositional Analyses
Before analyses, the vegetative components including roots were washed thoroughly under a jet of running tap water to remove the attached soil. Then, they were dipped in diluted HCl (1 mL concentrated HCL.L−1 water) [41]. Further washing was performed with de-ionized water. Sampled species were first identified as grasses or legumes, then the above- and belowground biomass yields were estimated in t. ha−1 [41]. Afterward, the roots were removed, and the clean leaf samples were dried in a hot air-circulating oven at 105 ℃ for 24 h until a constant weight is obtained. Then, they were girded for nutrient analysis [41].
The nitrogen content was estimated using the micro Kjeldahl method [42]. The phosphorus content was determined following the vanado-molybdo-phosphoric Acid yellow colorimetric method [43]. Briefly, the dissolved reactive phosphorus reacted with ammonium molybdate under acid conditions. Hence, the molybdo-phosphoric acid was formed, and a yellow vanado-molybdo-phosphoric acid was obtained in the presence of vanadium. This corresponds to the phosphorus concentration. Such concentration was detected at a wavelength of 470 nm using Thermo Spectronic Helios Gamma UV (Hellma model: 178.712-QS, flow cell: 10 mm light path, inner optical volume: 30 L), connected to a Kipp & Zonen BD112 recorder [43].
The potassium content was determined using the FLAPHO flame photometer method [44]. Briefly, the instrument was warmed up for 10 min and distilled water was fed to the instrument. Then, the indicators were adjusted to 0 (reading). The concentrated standard solution was aspirated, and the readout was adjusted to 90 (on the uppermost scale). Afterward, the distilled water was aspirated, and the instrument read 0. All standards and standard solutions were aspirated, and results were recorded. Then, the calibration curves were drawn; the potassium concentration corresponded to the abscissa, whereas the instrument readouts corresponded to the ordinate. Finally, the potassium concentration was noted.
Using the acid–alkali digestion method, the residue after acid and alkaline digestion (determined gravimetrically) corresponded to the crude fiber content [45]. The crude protein content was calculated after the determination of the leaf nitrogen via the micro Kjeldahl method. The leaf nitrogen content value was multiplied by a coefficient factor of 6.25 [46]. All compositional elements were expressed as percentage (%) dry matter.
4.6. Statistical Analysis
One-way ANOVA, Duncan, and Student’s t tests were applied for data analysis using the SPSS 25® program. A confidence level of 95% (p = 0.05) was adopted for all statistical tests. A comparison between regions districts was performed (different lower-case letters refer to a significant difference) in terms of AGB/BGB, R1, R2, P1, P2, and P3 (Table 1, Table 3, and Table 5). A comparison between villages of different districts was performed (different capital letters refer to a significant difference) as well as between AGB and BGB (different lowercase letters refer to a significant difference (Table 2, Table 4, and Table 6). In a similar vein, a comparison between grasses and legume types in terms of yield, and compositional elements (N, P, K, crude fiber and crude protein), was performed (different letters refer to a significant difference) (Table 7 and Table 8).
5. Conclusions
Kashmir Valley rangelands (northern, central and southern Kashmir regions) were investigated for their AGB, BGB, and total biomass (grasses/legumes) yields in three site types (grazed, protected, and seed-sown) along with their leaf nutritional profiles (N, P, K, crude fiber, and crude protein contents). Results showed an overall moderate grazing with a low to moderate effect on biomass. AGB, BGB, and total biomass yields were the highest in central Kashmir, followed by southern, and northern Kashmir. AGB and total biomass yields recorded the highest values in the protected sites of central Kashmir region, whereas, BGB yield scored the highest value in the protected sites of southern Kashmir region. On the other hand, Agrostis grass showed the highest crude fiber and crude protein contents among grasses found in the studied regions, whereas the highest crude fiber and crude protein contents among prominent legumes were recorded for red clover and white clover, respectively. Those findings concluded the successful management of Kashmir rangelands in protected sites, resulting in high biomass yields along with the considerable nutritional value of grasses and legumes.
Author Contributions
Conceptualization, J.A.M. and M.u.d.K.; methodology, J.A.M. and M.u.d.K.; software, P.K. and S.A.F.; validation, M.u.d.D., I.S., H.F.A., A.A.B., S.A.A., A.M.A., P.K. and S.A.F.; formal analysis, S.A.F.; investigation, S.A.F.; resources, H.F.A., A.A.B., S.A.A. and A.M.A.; data curation, S.A.F.; writing—original draft preparation, M.u.d.K. and S.A.F.; writing—review and editing, I.S., P.K. and S.A.F.; visualization, M.u.d.D., I.S., H.F.A., P.K. and S.A.F.; supervision, M.u.d.K. and S.A.F.; project administration, S.A.F.; funding acquisition, H.F.A. and S.A.F. All authors have read and agreed to the published version of the manuscript.
Funding
This work was funded by Institutional Fund Projects under grant No. (IFPIP: 394-130-1443), Ministry of Education in Saudi Arabia.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
All data used in the present study are included in the manuscript.
Acknowledgments
This research work was funded by Institutional Fund Projects under grant No. (IFPIP: 394-130-1443). The authors gratefully acknowledge technical and financial support provided by the Ministry of Education and King Abdulaziz University, DSR, Jeddah, Saudi Arabia.
Conflicts of Interest
The authors declare no conflict of interest.
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Figure 1.
AGB, BGB and total biomass yield (t. ha−1) in studied circles.
Table 1.
Comparison between northern Kashmir districts in terms of biomass production.
| Parameter | Kupwara | Baramulla | Bandipora | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | |
| AGB/BGB | 1.61a | 1.32b | 1.85a | 1.31b | 1.38a | 1.65c | 1.29b | 1.15c | 1.81b |
| R1 | 2.37a | 2.33a | 2.36a | ||||||
| R2 | 1.48a | 1.12b | 1.43b | ||||||
| P1 (%) | 100.00a | 77.78b | 66.67c | ||||||
| P2 (%) | 100.00a | 100.00a | 100.00a | ||||||
| P3 (%) | 100.00a | 33.33c | 66.67b | ||||||
AGB: above-ground biomass; BGB: below-ground biomass; R1: average ratio of protected sites biomass over grazed sites biomass (protected sites biomass/grazed sites biomass); R2: average ratio of seed-sown sites biomass over grazed sites biomass (seed-sown sites biomass/grazed sites biomass); P1: percentage of villages within district where ABG > BGB; P2: percentage of villages within district where protected sites biomass > grazed sites biomass; P3: percentage of villages within district where seed-sown sites biomass > grazed sites biomass. Values are means; means within the same row followed by different letters are significantly different at p ˂ 0.05 according to Duncan’s multiple range test.
Table 2.
Above (AGB) and below Ground Biomass (BGB) (t/ha) in the pasture of northern Kashmir.
| District | Village | Biomass Type | Grazed Sites | Protected Sites | Seed-Sown Sites | Average Biomass |
|---|---|---|---|---|---|---|
| Kupwara | Bangas Valley | AGB | 0.75Da | 1.46Da | 1.34Ea | 1.19Ea |
| BGB | 0.51Eb | 1.06Gb | 1.07Eb | 0.80Eb | ||
| Wogubal | AGB | 1.11Ca | 3.58Ca | 1.81Ca | 2.16Da | |
| BGB | 0.63Db | 2.53Cb | 0.81Fb | 1.32Cb | ||
| Rajwar | AGB | 4.26Ba | 5.98Ba | 4.83Aa | 5.02Ba | |
| BGB | 2.66Bb | 5.17Ab | 2.32Ab | 3.38Ab | ||
| Baramulla | Firozpur | AGB | 0.51Eb | 1.38Ea | 1.05Fb | 0.98Fb |
| BGB | 0.85Ca | 1.36Fb | 1.24Da | 1.15Da | ||
| Dragbah | AGB | 1.11Ca | 3.58Ca | 1.65Da | 2.11Da | |
| BGB | 0.63Db | 2.43Db | 0.85Fb | 1.30Cb | ||
| Gulmarg | AGB | 4.46Aa | 6.98Aa | 4.81Aa | 5.41Aa | |
| BGB | 2.82Ab | 4.17Bb | 2.22Bb | 3.07Bb | ||
| Bandipora | Ketsan | AGB | 0.51Eb | 1.28Fb | 1.09Fb | 0.96Fb |
| BGB | 0.85Ca | 1.46Ea | 1.24Da | 1.18Da | ||
| Awathwooth | AGB | 1.11Ca | 3.58Ca | 1.95Ba | 2.21Ca | |
| BGB | 0.63Db | 2.53Cb | 0.85Fb | 1.33Cb | ||
| Cithernaar | AGB | 4.26Ba | 5.98Ba | 4.81Aa | 5.01Ba | |
| BGB | 2.82Ab | 5.17Ab | 2.12Cb | 3.37Ab |
Values are means (3 replicates of each biomass type); means within the same column (comparison between villages in terms of AGB/BGB) followed by different capital letters are significantly different at p < 0.05 according to Duncan test; means within the same column (comparison between AGB and BGB within each village) followed by different lowercase letters are significantly different at p ˂ 0.05 according to Student’s t test.
Table 3.
Comparison between central Kashmir districts in terms of biomass production.
| Parameter | Ganderbal | Budgam | Srinagar | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | |
| AGB/BGB | 1.70b | 1.46c | 1.93b | 2.08a | 1.72a | 2.11a | 1.45c | 1.57b | 1.83c |
| R1 | 2.70a | 2.62b | 2.70a | ||||||
| R2 | 1.47b | 1.32c | 1.53a | ||||||
| P1 (%) | 100.00a | 100.00a | 100.00a | ||||||
| P2 (%) | 100.00a | 100.00a | 100.00a | ||||||
| P3 (%) | 100.00a | 100.00a | 100.00a | ||||||
AGB: above-ground biomass; BGB: below-ground biomass; R1: average ratio of protected sites biomass over grazed sites biomass (protected sites biomass/grazed sites biomass); R2: average ratio of seed-sown sites biomass over grazed sites biomass (seed-sown sites biomass/grazed sites biomass); P1: percentage of villages within district where ABG > BGB; P2: percentage of villages within district where protected sites biomass > grazed sites biomass; P3: percentage of villages within district where seed-sown sites biomass > grazed sites biomass. Values are means; means within the same row followed by different letters are significantly different at p ˂ 0.05 according to Duncan’s multiple range test.
Table 4.
Above (AGB) and belowground biomass (BGB) (t/ha) in the pasture of central Kashmir.
| District | Village | Biomass Type | Grazed Sites | Protected Sites | Seed-Sown Sites | Average Biomass |
|---|---|---|---|---|---|---|
| Ganderbal | Rayil | AGB | 0.85Ea | 1.56Ga | 1.34Fa | 1.25Ga |
| BGB | 0.54Gb | 1.36Fb | 1.09Db | 0.99Gb | ||
| Wangeth | AGB | 1.11Ca | 4.58Da | 1.95Da | 2.54Ea | |
| BGB | 0.63Fb | 2.93Db | 0.85Fb | 1.47Fb | ||
| Naranag | AGB | 4.26Ba | 6.98Ba | 4.81Ba | 5.35Ca | |
| BGB | 2.42Cb | 4.17Cb | 2.13Cb | 2.91Db | ||
| Budgam | Yousmarg | AGB | 0.94Da | 1.88Ea | 1.19Ga | 1.33Fa |
| BGB | 0.28Hb | 0.98Hb | 0.51Gb | 0.59Hb | ||
| Dodhpathri | AGB | 1.18Ca | 4.68Ca | 1.98Da | 2.61Da | |
| BGB | 0.72Eb | 2.97Db | 0.87Fb | 1.52Eb | ||
| Kanidajan | AGB | 4.76Aa | 7.99Aa | 5.88Aa | 6.21Aa | |
| BGB | 3.82Ab | 4.80Bb | 3.42Ab | 4.01Ab | ||
| Srinagar | Astanmarg | AGB | 0.73Fa | 1.78Fa | 1.41Ea | 1.31Fa |
| BGB | 0.55Gb | 1.16Gb | 1.09Db | 0.94Gb | ||
| Zahgemarg | AGB | 1.11Ca | 4.61Ca | 2.09Ca | 2.61Da | |
| BGB | 0.83Db | 2.83Eb | 0.95Eb | 1.54Eb | ||
| Chirenbal | AGB | 4.26Ba | 7.98Aa | 4.85Ba | 5.71Ba | |
| BGB | 2.52Bb | 5.17Ab | 2.42Bb | 3.37Bb |
Values are means (3 replicates of each biomass type); means within the same column (comparison between villages in terms of AGB/BGB) followed by different capital letters are significantly different at p < 0.05 according to Duncan test; means within the same column (comparison between AGB and BGB within each village) followed by different lowercase letters are significantly different at p ˂ 0.05 according to the Student’s t-test.
Table 5.
Comparison between southern Kashmir districts in terms of biomass production.
| Parameter | Anantnag | Shopian | Kulgam | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | Grazed Sites | Protected Sites | Seed-Sown Sites | |
| AGB/BGB | 1.71a | 1.18b | 1.82a | 1.31c | 1.38a | 1.69c | 1.43b | 1.13b | 1.71b |
| R1 | 2.38a | 2.19c | 2.23b | ||||||
| R2 | 1.43b | 1.31c | 1.47a | ||||||
| P1 (%) | 100.00a | 66.67b | 66.67b | ||||||
| P2 (%) | 100.00a | 100.00a | 100.00a | ||||||
| P3 (%) | 66.67b | 66.67b | 100.00a | ||||||
AGB: above-ground biomass; BGB: below-ground biomass; R1: average ratio of protected sites biomass over grazed sites biomass (protected sites biomass/grazed sites biomass); R2: average ratio of seed-sown sites biomass over grazed sites biomass (seed-sown sites biomass/grazed sites biomass); P1: percentage of villages within district where ABG > BGB; P2: percentage of villages within district where protected sites biomass > grazed sites biomass; P3: percentage of villages within district where seed-sown sites biomass > grazed sites biomass. Values are means; means within the same row followed by different letters are significantly different at p < 0.05 according to Duncan’s multiple range test.
Table 6.
Above (AGB) and belowground biomass (BGB) (t/ha) in the pasture of southern Kashmir.
| District | Village | Biomass Type | Grazed Sites | Protected Sites | Seed-Sown Sites | Average Biomass |
|---|---|---|---|---|---|---|
| Anantnag | Daksum | AGB | 0.79Ga | 1.51Fa | 1.32Fa | 1.21Ea |
| BGB | 0.54Fb | 1.48Gb | 1.09Eb | 1.03Eb | ||
| Alhan | AGB | 1.25Ea | 3.74Ca | 1.98Da | 2.32Ca | |
| BGB | 0.65Eb | 2.58Db | 0.91Fb | 1.38Cb | ||
| Aru Valley | AGB | 4.69Aa | 5.98Ba | 4.81Ba | 5.16Ba | |
| BGB | 2.68Bb | 5.57Ab | 2.32Bb | 3.52Ab | ||
| Shopian | Dabjan | AGB | 0.59Hb | 1.41Gb | 1.05Gb | 1.01Fb |
| BGB | 0.85Ca | 1.49Ga | 1.24Da | 1.19Da | ||
| Hirpora | AGB | 1.21Fa | 3.68Da | 1.85Ea | 2.24Da | |
| BGB | 0.77Db | 2.43Eb | 0.92Fb | 1.37Cb | ||
| Kaller | AGB | 4.70Aa | 6.98Aa | 4.91Ba | 5.51Aa | |
| BGB | 2.82Ab | 4.17Cb | 2.22Cb | 3.07Bb | ||
| Kulgam | Astanmarg | AGB | 0.51Hb | 1.28Hb | 1.09Gb | 0.96Gb |
| BGB | 0.88Ca | 1.56Fa | 1.25Da | 1.23Da | ||
| Zahgemarg | AGB | 1.31Da | 3.58Ea | 2.05Ca | 2.31Ca | |
| BGB | 0.63Eb | 2.53Db | 1.05Eb | 1.40Cb | ||
| Chirenbal | AGB | 4.56Ba | 5.98Ba | 5.81Aa | 5.45Aa | |
| BGB | 2.82Ab | 5.19Bb | 2.52Ab | 3.51Ab |
Values are means (3 replicates of each biomass type); means within the same column (comparison between villages in terms of AGB/BGB) followed by different capital letters are significantly different at p < 0.05 according to Duncan test; means within the same column (comparison between AGB and BGB within each village) followed by different lowercase letters are significantly different at p< 0.05 according to the Student’s t test.
Table 7.
Yield and leaf nutrient profile of prominent grasses.
| Grasses | Yield (t. ha−1) | N (%) | p (%) | K (%) | Crude Fiber (%) | Crude Protein (%) |
|---|---|---|---|---|---|---|
| Dactylis glomerata (Orchard grass) | 12.50 ± 0.6a | 1.51 ± 0.04b | 0.07 ± 0.01d | 0.31 ± 0.04c | 15.30 ± 0.6b | 10.30 ± 0.5a |
| Festuca arundinacea (Tall fescue grass) | 11.80 ± 0.6ab | 1.22 ± 0.03c | 0.18 ± 0.02c | 0.41 ± 0.05ab | 11.20 ± 0.6b | 7.50 ± 0.3b |
| Lolium perenne (Perennial rye grass) | 10.23 ± 0.5b | 1.29 ± 0.03c | 0.29 ± 0.03b | 0.44 ± 0.05a | 35.12 ± 0.8a | 5.10 ± 0.2c |
| Phleum pratense (Timothy grass) | 8.57 ± 0.4c | 1.81 ± 0.05a | 0.21 ± 0.03c | 0.39 ± 0.04b | 31.20 ± 0.8a | 9.50 ± 0.4a |
| Agrostis alba (Agrostis grass) | 3.51 ± 0.2d | 1.23 ± 0.03c | 0.34 ± 0.04a | 0.23 ± 0.03d | 34.20 ± 0.8a | 10.40 ± 0.5a |
Values are means (3 replicates of each grass); means within the same column followed by different letters are significantly different at p< 0.05 according to Duncan’s multiple range test.
Table 8.
Yield and leaf nutrient profile of prominent legumes.
| Legumes | Yield (t. ha−1) | N (%) | p (%) | K (%) | Crude Fiber (%) | Crude Protein (%) |
|---|---|---|---|---|---|---|
| Trifolium pratense (Red clover) | 25.40 ± 0.6a | 1.69 ± 0.05a | 0.33 ± 0.04a | 0.45 ± 0.05a | 29.89 ± 0.7a | 22.70 ± 0.6a |
| Trifolium repens (White clover) | 24.20 ± 0.6ab | 1.23 ± 0.03c | 0.34 ± 0.04a | 0.25 ± 0.03c | 33.12 ± 0.8a | 21.10 ± 0.6a |
| Medicago sativa L. (Lucerne) | 23.40 ± 0.6b | 1.67 ± 0.05a | 0.31 ± 0.04a | 0.43 ± 0.05a | 11.50 ± 0.6b | 18.80 ± 0.5b |
| Onobrychis viciifolia (Sainfoin) | 7.50 ± 0.3d | 1.19 ± 0.02c | 0.34 ± 0.04a | 0.23 ± 0.03c | 10.20 ± 0.5b | 15.40 ± 0.5c |
| Securigera varia (Crown vetch) | 9.30 ± 0.4c | 1.47 ± 0.04b | 0.11 ± 0.02b | 0.35 ± 0.04b | 9.23 ± 0.4b | 11.56 ± 0.5d |
Values are means (3 replicates of each grass); means within the same column followed by different letters are significantly different at p< 0.05 according to Duncan’s multiple range test.
Table 9.
Spatial distribution of studied sites.
| Zone Name | District Name | Village Name | District Coordinates | District Altitude |
|---|---|---|---|---|
| Northern Kashmir | Kupwara | Bangus Valley, Wogubal, Rajwar | 34°18′–34°47′ N, 73°45′–74°30′ E | 2000–3500 m |
| Baramulla | Firozpur, Dragbah, Gulmarg | 34°11′–34°19′ N, 74°21′–74°36′ E | 1630–2085 m | |
| Bandipora | Ketsan, Awathwooth, Cithernaar | 34°25′–34°41′ N, 74°39′–74°65′ E | 2700–4800 m | |
| Central Kashmir | Srinagar | Baedhmargh Reshipora, Astanmarg Dara, Syedpora Bla | 34°05′–34°08′ N, 74°50′–74°83′ E | 1450–3942 m |
| Budgam | Yousmarg, Dodhpathri, Kanidajan | 33°93′–34°02′ N, 74°69′–74°79′ E | 2000–2730 m | |
| Ganderbal | Rayil, Wangeth, Naranag | 33°44′–33°73′ N, 75°09′–75°15′ E | 1716–3397 m | |
| Southern Kashmir | Anantnag | Daksum, Ahlan, Aru Valley | 33°36′–34°25′ N, 75°02′–75°59′ E | 1600–1723 m |
| Shopian | Dabjan, Hirpora, Kaller | 33°43′–33°72′ N, 74°50′–74°83′ E | 1650–4720 m | |
| Kulgam | Astanmarg, Zahgemarg, Chirenbal | 33°55′–33°78′ N, 74°90′–75°17′ E | 1740–4800 m |
Table 10.
Diversity of grasses and legumes in the studied Kashmir regions.
| Zone | Grasses | Legumes |
|---|---|---|
| Northern Kashmir | Dactylis glomerata | Trifolium pratense |
| Festuca arundinacea | Trifolium repens | |
| Lolium perenne | Onobrychis viciifolia | |
| Phleum pratense | Medicago sativa | |
| Bromus unioloides | Securigera varia | |
| Phalaris spp. | ||
| Poa pratensis | ||
| Lolium multiflorum | ||
| Agrostis alba | ||
| Avena sativa | ||
| Central Kashmir | Dactylis glomerata | Trifolium alexandrinum |
| Festuca arundinacea | Stylosanthus hamata | |
| Lolium perenne | Macroptilium atropupreum | |
| Dicanthium annulatum | Trifolium pratense | |
| Chloris gayana | Trifolium repens | |
| Chrysopogon fulvus | Onobrychis viciifolia | |
| Heteropogon contortus | Medicago sativa | |
| Agrostis alba | Securigera varia | |
| Setaria spp. | ||
| Avena sativa | ||
| Phleum pratense | ||
| Southern Kashmir | Dactylis glomerata | Trifolium pratense |
| Festuca arundinacea | Trifolium repens | |
| Lolium perenne | Onobrychis viciifolia | |
| Agrostis alba | Medicago sativa | |
| Phleum pratense | Securigera varia | |
| Avena sativa | ||
| Setaria spp. |
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Mugloo, J.A.; Khanday, M.u.d.; Dar, M.u.d.; Saleem, I.; Alharby, H.F.; Bamagoos, A.A.; Alghamdi, S.A.; Abdulmajeed, A.M.; Kumar, P.; Abou Fayssal, S. Biomass and Leaf Nutrition Contents of Selected Grass and Legume Species in High Altitude Rangelands of Kashmir Himalaya Valley (Jammu & Kashmir), India. Plants 2023, 12, 1448. https://doi.org/10.3390/plants12071448
AMA Style
Mugloo JA, Khanday Mud, Dar Mud, Saleem I, Alharby HF, Bamagoos AA, Alghamdi SA, Abdulmajeed AM, Kumar P, Abou Fayssal S. Biomass and Leaf Nutrition Contents of Selected Grass and Legume Species in High Altitude Rangelands of Kashmir Himalaya Valley (Jammu & Kashmir), India. Plants. 2023; 12(7):1448. https://doi.org/10.3390/plants12071448
Chicago/Turabian StyleMugloo, Javed A., Mehraj ud din Khanday, Mehraj ud din Dar, Ishrat Saleem, Hesham F. Alharby, Atif A. Bamagoos, Sameera A. Alghamdi, Awatif M. Abdulmajeed, Pankaj Kumar, and Sami Abou Fayssal. 2023. "Biomass and Leaf Nutrition Contents of Selected Grass and Legume Species in High Altitude Rangelands of Kashmir Himalaya Valley (Jammu & Kashmir), India" Plants 12, no. 7: 1448. https://doi.org/10.3390/plants12071448
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