Plant Genotype Shapes the Soil Nematode Community in the Rhizosphere of Tomatoes with Different Resistance to Meloidognye incognita
Abstract
:1. Introduction
2. Results
2.1. Alpha Diversity Analysis
2.2. Beta Diversity Analysis
2.3. Comparison of Ecological Indices of Three Soil Nematode Communities and Disease Index
2.4. Effect of Root Exudates on Soil Nematode Community
2.5. Effects of Root Exudates on M. incognita
3. Discussion
4. Materials and Methods
4.1. Tomato and M. incognita Materials
4.2. Collection of Root Exudates and Gas Chromatography/Mass Spectrometry Analysis
4.3. Tomato Planting in the Greenhouse
4.4. Soil Nematode Isolation and DNA Extraction
4.5. PCR Amplification and Pyrosequencing
4.6. Bioinformation Analysis
4.7. Ecological Index Analysis
4.8. Effect of Root Exudates on Mortality Rate of M. incognita J2
4.9. Effect of Root Exudates on M. incognita Egg-Hatching
4.10. Effect of Root Exudates on the Chemotaxis of M. incognita J2
4.11. Data Analysis
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Root Exudates | Susceptible Cultivar | Tolerant Cultivar | Resistant Cultivar |
---|---|---|---|
Tetradecane | 7.28 ± 0.07 a | 6.85 ± 0.08 b | 8.86 ± 0.05 c |
N-dodecane | 4.94 ± 0.05 a | 4.41 ± 0.04 b | 4.31 ± 0.05 c |
1,3-ditert-butylbenzene | 1.61 ± 0.02 a | 2.26 ± 0.02 b | 3.06 ± 0.06 c |
Heptadecane | 3.06 ± 0.05 a | 3.63 ± 0.03 b | 5.85 ± 0.06 c |
N-eicosane | 9.75 ± 0.04 a | 12.20 ± 0.09 b | 12.60 ± 0.03 c |
2,6-ditert-butylp-cresol | 16.44 ± 0.04 a | 16.18 ± 0.05 b | 14.94 ± 0.05 c |
Palmitamide | 4.78 ± 0.04 a | 4.14 ± 0.03 b | 3.96 ± 0.02 c |
Oleamide | 26.62 ± 0.14 a | 25.15 ± 0.05 b | 22.29 ± 0.08 c |
1-naphthyl aminobenzene | 1.27 ± 0.03 a | 1.47 ± 0.03 b | 1.50 ± 0.05 b |
Stearamide | 4.05 ± 0.02 a | 4.27 ± 0.79 a | 3.73 ± 0.05 a |
Undecane | 2.49 ± 0.05 a | 2.91 ± 0.03 b | 2.20 ± 0.04 c |
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Wang, X.; Wang, C.; Chen, R.; Wang, W.; Wang, D.; Tian, X. Plant Genotype Shapes the Soil Nematode Community in the Rhizosphere of Tomatoes with Different Resistance to Meloidognye incognita. Plants 2023, 12, 1528. https://doi.org/10.3390/plants12071528
Wang X, Wang C, Chen R, Wang W, Wang D, Tian X. Plant Genotype Shapes the Soil Nematode Community in the Rhizosphere of Tomatoes with Different Resistance to Meloidognye incognita. Plants. 2023; 12(7):1528. https://doi.org/10.3390/plants12071528
Chicago/Turabian StyleWang, Xiangmei, Chaoyan Wang, Ru Chen, Wenxing Wang, Diandong Wang, and Xueliang Tian. 2023. "Plant Genotype Shapes the Soil Nematode Community in the Rhizosphere of Tomatoes with Different Resistance to Meloidognye incognita" Plants 12, no. 7: 1528. https://doi.org/10.3390/plants12071528