The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Analysis of STRP Levels and STRP Expression under Salt Stress
2.2. STRP Overexpression
2.3. Physiological Responses to Salt Stress of the Strp Mutant and STRP OE Plants
2.4. Biochemical Responses to Salt Stress in the Strp Mutant and STRP OE Plants
2.5. ABA Accumulation and NCED3 Expression under Salt Stress in wt, strp, and STRP OE Plants
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Vector Construction and Agrobacterium–Mediated Transformation of A. thaliana Plants
4.3. Growth Conditions and Salt Stress Treatment
4.4. Germination Assay, Primary Root Elongation, and Leaf Area Measurement
4.5. Whole Cellular Extract Preparation
4.6. Western Blot
4.7. Ascorbate Peroxidase Activity Assay
4.8. Gene Expression Analysis
4.9. Lipid Peroxidation and Relative Electrolyte Conductivity
4.10. Determination of Free Proline
4.11. Total Chlorophyll Assay
4.12. Determination of Hydrogen Peroxide by DAB Staining
4.13. HPLC Analysis of Abscisic Acid
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fiorillo, A.; Manai, M.; Visconti, S.; Camoni, L. The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana. Plants 2023, 12, 1704. https://doi.org/10.3390/plants12081704
Fiorillo A, Manai M, Visconti S, Camoni L. The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana. Plants. 2023; 12(8):1704. https://doi.org/10.3390/plants12081704
Chicago/Turabian StyleFiorillo, Anna, Michela Manai, Sabina Visconti, and Lorenzo Camoni. 2023. "The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana" Plants 12, no. 8: 1704. https://doi.org/10.3390/plants12081704