Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways
Abstract
:1. Introduction
2. Results
2.1. cDNA Libraries Aligning on Reference Transcriptome
2.2. Differential Expression and Gene Ontology Analysis of Genes of Control and UV-B-Treated Peach Fruit
2.3. Cellular Function Clustering of DEGs Exposed to UV-B
2.4. Co-Expression Network Analysis of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Material and UV-B Treatment
4.2. RNA Isolation
4.3. cDNA Libraries Preparation
4.4. Differential Expression Analysis
4.5. Gene Ontology (GO) and DEGs Functional Classification
4.6. Gene Co-Expression Network Analysis and Metabolic Data Correlation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Library | Replicate | Raw Reads Per Library (N) | Trimmed Reads Per Library (N) | Aligned Reads on P. persica Reference Transcriptome (N) | Aligned Reads on P. persica Reference Transcriptome (%) |
---|---|---|---|---|---|
CTR_1 h | 1 | 33,257,571 | 32,949,871 | 31,328,689 | 95.08 |
2 | 28,285,172 | 28,032,925 | 26,375,914 | 94.09 | |
3 | 27,168,547 | 26,980,657 | 25,341,162 | 93.92 | |
UV-B_1 h | 1 | 27,439,039 | 27,181,626 | 25,618,232 | 94.25 |
2 | 27,978,969 | 27,717,323 | 26,033,386 | 93.92 | |
3 | 30,422,616 | 30,161,462 | 28,388,389 | 94.12 | |
CTR_3 h | 1 | 26,980,674 | 26,692,135 | 25,040,323 | 93.81 |
2 | 28,983,969 | 28,740,615 | 26,775,717 | 93.16 | |
3 | 24,796,737 | 24,601,286 | 22,984,308 | 93.43 | |
UV-B_3 h | 1 | 28,917,100 | 28,587,951 | 27,065,772 | 94.68 |
2 | 31,460,791 | 31,111,037 | 29,191,493 | 93.83 | |
3 | 23,325,335 | 23,126,890 | 21,689,653 | 93.79 |
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Santin, M.; Simoni, S.; Vangelisti, A.; Giordani, T.; Cavallini, A.; Mannucci, A.; Ranieri, A.; Castagna, A. Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways. Plants 2023, 12, 1818. https://doi.org/10.3390/plants12091818
Santin M, Simoni S, Vangelisti A, Giordani T, Cavallini A, Mannucci A, Ranieri A, Castagna A. Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways. Plants. 2023; 12(9):1818. https://doi.org/10.3390/plants12091818
Chicago/Turabian StyleSantin, Marco, Samuel Simoni, Alberto Vangelisti, Tommaso Giordani, Andrea Cavallini, Alessia Mannucci, Annamaria Ranieri, and Antonella Castagna. 2023. "Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways" Plants 12, no. 9: 1818. https://doi.org/10.3390/plants12091818