Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System
Abstract
:1. Introduction
2. Results
2.1. Photosynthetic Characteristic Changes in Response to Maize–Peanut Intercropping
2.2. Primary Transcriptome and Proteome Data Analyses
2.3. The mRNA and Protein Expression Changes in the Genome under Maize–Peanut Intercropping
2.4. Photosynthetic Response Transcriptome Differences under Maize–Peanut Intercropping
2.5. Photosynthetic Response Proteome Differences under Maize–Peanut Intercropping
2.6. Transcriptional Expression Analysis by qRT-PCR
2.7. String-Based Protein–Protein Interaction (PPI) Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Growth Condition, Treatments, and Sampling
4.2. RNA Extraction, Library Construction, and Sequencing
4.3. Protein Extraction, Concentration Determination, Enzymatic Digestion, and iTRAQ Reagent Labeling
4.4. Chromatography, LC–MS/MS Analysis, and Raw Data Analysis
4.5. Total RNA Extraction and qPCR
4.6. Assay of Photo Flux Density and Photosynthetic Characteristics
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Ma, C.; Feng, Y.; Wang, J.; Zheng, B.; Wang, X.; Jiao, N. Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System. Plants 2024, 13, 65. https://doi.org/10.3390/plants13010065
Ma C, Feng Y, Wang J, Zheng B, Wang X, Jiao N. Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System. Plants. 2024; 13(1):65. https://doi.org/10.3390/plants13010065
Chicago/Turabian StyleMa, Chao, Yalan Feng, Jiangtao Wang, Bin Zheng, Xiaoxiao Wang, and Nianyuan Jiao. 2024. "Integrative Physiological, Transcriptome, and Proteome Analyses Provide Insights into the Photosynthetic Changes in Maize in a Maize–Peanut Intercropping System" Plants 13, no. 1: 65. https://doi.org/10.3390/plants13010065