Comparative Metabolome and Transcriptome Analyses Reveal Differential Enrichment of Metabolites with Age in Panax notoginseng Roots
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Tissue Collection
2.2. Metabolome Analysis
2.3. Transcriptome Analysis
2.4. Statistical Analysis
3. Results
3.1. Metabolome of P. notoginseng (PN2 vs. PN3)
3.1.1. Differential Metabolites
3.1.2. KEGG Differential Metabolites Enrichment Analysis (PN2 vs. PN3)
3.1.3. KEGG Pathways Enrichment Analysis
3.2. Transcriptome of Panax notoginseng (PN2 vs. PN3)
Differentially Expressed Gene Analysis
3.3. Gene Ontology Pathways Enrichment (GO) Analysis
3.4. Combined Metabolome and Transcriptome KEGG Enrichment Analysis
3.5. Expression Changes in Various Plants Secondary Metabolic Pathways
3.6. Expression Changes in Phytohormone Signaling Pathways
4. Discussion
4.1. Metabolomic Analysis
4.2. Transcriptome Analysis and Expression Pathways
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Yan, X.; Zhang, A.; Guan, Y.; Jiao, J.; Ghanim, M.; Zhang, Y.; He, X.; Shi, R. Comparative Metabolome and Transcriptome Analyses Reveal Differential Enrichment of Metabolites with Age in Panax notoginseng Roots. Plants 2024, 13, 1441. https://doi.org/10.3390/plants13111441
Yan X, Zhang A, Guan Y, Jiao J, Ghanim M, Zhang Y, He X, Shi R. Comparative Metabolome and Transcriptome Analyses Reveal Differential Enrichment of Metabolites with Age in Panax notoginseng Roots. Plants. 2024; 13(11):1441. https://doi.org/10.3390/plants13111441
Chicago/Turabian StyleYan, Xinru, Ao Zhang, Yiming Guan, Jinlong Jiao, Murad Ghanim, Yayu Zhang, Xiahong He, and Rui Shi. 2024. "Comparative Metabolome and Transcriptome Analyses Reveal Differential Enrichment of Metabolites with Age in Panax notoginseng Roots" Plants 13, no. 11: 1441. https://doi.org/10.3390/plants13111441