In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil)
Abstract
:1. Introduction
2. Results
2.1. Effects of Tulsi Leaf Extracts on Organogenesis
2.2. Differences in the Mode of Regeneration of N. tabacum Explants
2.3. Effect of the Extracts on PGRs
2.3.1. Endogenous Levels of PGRs in Tulsi Leaf Extracts
2.3.2. Interplay among PGRs
Indole-3-Acetic Acid (IAA)
Abscisic Acid (ABA)
Gibberellic Acid (GA3)
6-Benzylaminopurine (BA)
Zeatin
6-(γ, γ-Dimethylallylamino)Purine (2iP)
Jasmonic Acid (JA)
2.3.3. Interaction between Zeatin and Abscisic Acid in Tobacco Leaf Explants
3. Discussion
4. Materials and Methods
4.1. Growth of Plant Materials and Preparation of Extracts
4.2. Nodal Culture Establishment of Tobacco Explants
Tobacco Leaf Disc Explants Culture and Sample Collection
4.3. Detection and Quantification of Hormones
4.3.1. Sample Collection and Extraction
4.3.2. Separation and Quantification
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Treatment | Average Number of Regenerants/Explant | Average Number of Shoots Greater than 1 cm/Explant | Average Number of Embryo-Like Structures |
---|---|---|---|
Control | 6.14 ± 0.5 c | 6.14 ± 0.5 c | 0 c |
1% Tulsi leaf extract | 9.60 ± 0.5 b | 8.75 ± 0.5 b | 0.98 ± 0.5 cb |
10% Tulsi leaf extract | 12.58 ± 0.5 a | 10.58 ± 0.5 a | 2.50 ± 0.5 a |
20% Tulsi leaf extract | 13.07 ± 0.5 a | 12.05 ± 0.5 a | 1.53 ± 0.5 b |
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Vongnhay, V.; Shukla, M.R.; Ayyanath, M.-M.; Sriskantharajah, K.; Saxena, P.K. In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil). Plants 2024, 13, 2002. https://doi.org/10.3390/plants13142002
Vongnhay V, Shukla MR, Ayyanath M-M, Sriskantharajah K, Saxena PK. In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil). Plants. 2024; 13(14):2002. https://doi.org/10.3390/plants13142002
Chicago/Turabian StyleVongnhay, Vanessa, Mukund R. Shukla, Murali-Mohan Ayyanath, Karthika Sriskantharajah, and Praveen K. Saxena. 2024. "In Vitro Morphogenesis of Tobacco: Modulation of Endogenous Growth Regulators by Tulsi (Holy Basil)" Plants 13, no. 14: 2002. https://doi.org/10.3390/plants13142002