Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple)

Ma, Jingze; Fan, Junjun; Zhang, Wangxiang; Zhou, Ruomiao; Shen, Yiting; Peng, Qin; Li, Huimin; Lei, Cong

doi:10.3390/plants13152080

Open AccessArticle

Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple)

by

Jingze Ma

^1,2,3,†,

Junjun Fan

^2,*,†

,

Wangxiang Zhang

^1,3,*,

Ruomiao Zhou

¹,

Yiting Shen

¹,

Qin Peng

¹

,

Huimin Li

¹ and

Cong Lei

¹

College of Forestry, Nanjing Forestry University, Nanjing 210037, China

²

College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China

³

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Plants 2024, 13(15), 2080; https://doi.org/10.3390/plants13152080

Submission received: 1 July 2024 / Revised: 20 July 2024 / Accepted: 25 July 2024 / Published: 26 July 2024

(This article belongs to the Section Horticultural Science and Ornamental Plants)

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Abstract

Malus ‘Baiyun’ (registration no. 20210210), a new crabapple cultivar, was registered in 2021 by the Nanjing Forestry Unversity. However, the difficult rooting has greatly limited the production of high-quality M. ‘Baiyun’ in industrialization development. There is thus a pressing need to develop an organogenesis protocol for the in vitro propagation of M. ‘Baiyun’ to alleviate a shortage of high-quality M. ‘Baiyun’ seedlings. The results showed that choosing the apical bud in mid-March was an excellent explant material. To promote proliferation, the highest proliferation (6.27) of apical shoots was cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg·L⁻¹ 6-benzylaminopurine(6-BA) + 0.05 mg·L⁻¹ indole-3-butyric acid (IBA). Subsequently, a 100% rooting rate, average number of roots per shoot of 6.2 and maximum length of roots of 4.96 cm were obtained on half-strength Murashige and Skoog (1/2 MS) medium with the application of 0.5 mg·L⁻¹ naphthaleneacetic acid (NAA) or 0.6 mg·L⁻¹ NAA + 0.7 mg·L⁻¹ IBA. Additionally, thick and lateral roots were obtained with 0.6 mg·L⁻¹ NAA + 0.7 mg·L⁻¹ IBA. Our study is the first to establish an effective organogenesis protocol for new crabapple cultivars using stem segments.

Keywords: Malus ‘Baiyun’; tissue culture; ornamental crabapple; shoot proliferation; rooting

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MDPI and ACS Style

Ma, J.; Fan, J.; Zhang, W.; Zhou, R.; Shen, Y.; Peng, Q.; Li, H.; Lei, C. Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple). Plants 2024, 13, 2080. https://doi.org/10.3390/plants13152080

AMA Style

Ma J, Fan J, Zhang W, Zhou R, Shen Y, Peng Q, Li H, Lei C. Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple). Plants. 2024; 13(15):2080. https://doi.org/10.3390/plants13152080

Chicago/Turabian Style

Ma, Jingze, Junjun Fan, Wangxiang Zhang, Ruomiao Zhou, Yiting Shen, Qin Peng, Huimin Li, and Cong Lei. 2024. "Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple)" Plants 13, no. 15: 2080. https://doi.org/10.3390/plants13152080

APA Style

Ma, J., Fan, J., Zhang, W., Zhou, R., Shen, Y., Peng, Q., Li, H., & Lei, C. (2024). Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple). Plants, 13(15), 2080. https://doi.org/10.3390/plants13152080

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Tissue Culture Response and In Vitro Plant Regeneration of Malus ‘Baiyun’ (a New Cultivar of Ornamental Crabapple)

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