Heterologous Expression of Chrysanthemum TCP Transcription Factor CmTCP13 Enhances Salinity Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of CmTCP13
2.2. Subcellular Localization of CmTCP13
2.3. Transcript Profile of CmTCP13 in Response to Salinity
2.4. Cis-Acting Regulatory Element Analysis of CmTCP13 Promoter Sequence
2.5. CmTCP13 Overexpression in Arabidopsis Enhanced Salinity Tolerance
2.6. CmTCP13 Overexpression Altered the Expression of Stress-Responsive Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Salinity Stress Treatments
4.2. Isolation and Sequence Analysis of CmTCP13
4.3. Subcellular Localization of CmTCP13
4.4. Ectopic Expression of CmTCP13 in Arabidopsis
4.5. Cis-Acting Element Analysis in the Promoter Region
4.6. Salinity Tolerance Assay of Transgenic Arabidopsis Plants
4.7. Measurements of Electrolyte Leakage and Chlorophyll Content
4.8. qRT-PCR Analyses
4.9. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Motif Name | Motif Sequence | Function | No. | Position |
---|---|---|---|---|
ABRE | ACGTG/CACGTG/GCAACGTGTC | Abscisic acid response element | 5 | 1704, −2123, −1780, −1778, 1781 |
ARE | AAACCA | Anaerobic-responsive element | 6 | 195, −1655, −1191, 1903, −871, 1536 |
ATC-motif | AGTAATCT | Light-responsive element | 1 | 1722 |
AuxRR-core | GGTCCAT | Auxin response element | 1 | 555 |
Box 4 | ATTAAT | Light-responsive element | 1 | 905 |
Box III | atCATTTTCACt | Protein binding site | 1 | 291 |
CGTCA-motif | CGTCA | MeJA-responsive element | 2 | 75, −1715 |
ERE | ATTTTAAA | Ethylene-responsive element | 1 | 636 |
G-box | CACGTT/CACGTG/ACACGTGGC/TACGTG | Light-responsive element | 4 | −1703, −1780, 1799, −2123 |
GA-motif | ATAGATAA | Light-responsive element | 1 | 606 |
GATA-motif | AAGATAAGATT/AAGGATAAGG | Light-responsive element | 3 | −211, 2302, −2164 |
GT-1 motif | GAAAAA | Salinity stress-inducible element | 7 | 95, 425, 1385, 2048, −294, −1145, −2342 |
HD-Zip 3 | GTAAT(G/C)ATTAC | Protein binding site | 1 | 701 |
I-box | AGATAAGG/cCATATCCAAT | Part of a light-responsive element | 2 | 239, −1832 |
LTR | CCGAAA | Low-temperature response element | 1 | 423 |
MBS | CAACTG | MYB binding site involved in drought-inducibility | 1 | 1267 |
MYB | TAACCA/CAACCA/CAACAG | MYB/YB recognition site | 5 | −1027, −2065, −1844, −1840, −2003 |
MYC | CATGTG | MYC/YC recognition site | 1 | 1283 |
P-box | CCTTTTG | Gibberellin-responsive element | 1 | 695 |
TCA-element | CCATCTTTTT | Salicylic acid-responsive element | 1 | 289 |
TGA-element | AACGAC | Auxin-responsive element | 1 | −44 |
TGACG-motif | TGACG | MeJA-responsive element | 2 | 5, −5 |
WUN-motif | AAATTTCCT | Wound-responsive element | 1 | −111 |
Circadian | CAAAGATATC | Circadian regulation element | 1 | −214 |
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Chong, X.; Liu, Y.; Li, P.; Wang, Y.; Zhou, T.; Chen, H.; Wang, H. Heterologous Expression of Chrysanthemum TCP Transcription Factor CmTCP13 Enhances Salinity Tolerance in Arabidopsis. Plants 2024, 13, 2118. https://doi.org/10.3390/plants13152118
Chong X, Liu Y, Li P, Wang Y, Zhou T, Chen H, Wang H. Heterologous Expression of Chrysanthemum TCP Transcription Factor CmTCP13 Enhances Salinity Tolerance in Arabidopsis. Plants. 2024; 13(15):2118. https://doi.org/10.3390/plants13152118
Chicago/Turabian StyleChong, Xinran, Yanan Liu, Peiling Li, Yue Wang, Ting Zhou, Hong Chen, and Haibin Wang. 2024. "Heterologous Expression of Chrysanthemum TCP Transcription Factor CmTCP13 Enhances Salinity Tolerance in Arabidopsis" Plants 13, no. 15: 2118. https://doi.org/10.3390/plants13152118