Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Varieties from South China
Abstract
:1. Introduction
2. Results
2.1. Variability of Resistance to Rice Blast
2.2. Identification and Analysis of Rice Blast Resistance Genes among the Backbone Rice Varieties in South China
2.3. Correlation Analysis of Gene Combination Distributions and Resistance Phenotypes
3. Discussion
3.1. Distribution and Manifestation of Blast Resistance Genes in Rice Varieties
3.2. Gene Pyramiding for Broad-Spectrum Rice Blast Resistance
4. Materials and Methods
4.1. Materials and Strains
4.2. Resistance Identification
4.3. Rice Blast Resistance Gene Detection
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Number | Germplasm Origin | Rice Blast Resistance Gene | Blast Resistance Level |
---|---|---|---|
23P-1 | Sichuan | Pia+Pid2+Pid3 | MR |
23P-2 | Sichuan | Pia+Pid3 | MS |
23P-3 | Sichuan | Pid3+Pita | MR |
23P-4 | Sichuan | Pia+Pid2+Pid3+Pigm | R |
23P-5 | Sichuan | Pia+Pid2+Pid3+Pigm+pikh | R |
23P-6 | Sichuan | Pia+Pid3+pikh | MS |
23P-7 | Hubei | Pi1+Pi5 | MS |
23P-8 | Hubei | Pi2+Pid2+Pid3 | MR |
23P-9 | Hunan | Pi2+Pi5+Pita | MS |
23P-10 | Hunan | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-11 | Hunan | Pia | MS |
23P-12 | Hunan | Pi5+Pi9+Pid2+Pid3 | MR |
23P-13 | Hunan | Pi5+Pi9+Pia+Pid2+Pid3 | R |
23P-14 | Hunan | Pi5 | MR |
23P-15 | Hunan | Pia+Pid2+Pid3 | MS |
23P-16 | Jiangxi | Pi2+Pi5 | MS |
23P-17 | Jiangxi | Pid2+Pid3 | MS |
23P-18 | Jiangxi | Pi2+Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-19 | Anhui | Pia+Pid2+Pid3 | MS |
23P-20 | Anhui | Pia+Pigm | MR |
23P-21 | Fujian | Pi2+Pid2+Pid3 | R |
23P-22 | Zhejiang | Pi5+Pia+Pid2+Pid3+Pita | R |
23P-23 | Zhejiang | Pi5+Pia | R |
23P-24 | Zhejiang | Pi2+Pi5+Pita | R |
23P-25 | Zhejiang | Pi2+Pi5 | MR |
23P-26 | Zhejiang | Pi5+Pid2+Pid3+Pita | MR |
23P-27 | Zhejiang | Pid2+Pid3+Pita | MR |
23P-28 | Zhejiang | Pia+Pid2+Pid3 | MS |
23P-29 | Zhejiang | Pi5+Pid2+Pid3 | MS |
23P-30 | Zhejiang | Pia+Pid2+Pid3 | R |
23P-31 | Zhejiang | Pi5+Pid3 | MS |
23P-32 | Zhejiang | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-33 | Zhejiang | Pi5+Pia+Pid2+Pid3 | MR |
23P-34 | Zhejiang | Pi2+Pi5 | MR |
23P-35 | Zhejiang | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-36 | Zhejiang | Pia+Pid2+Pid3 | MS |
23P-37 | Zhejiang | Pia | MR |
23P-38 | Zhejiang | Pi2+Pi5+Pia+Pita | MR |
23P-39 | Zhejiang | Pi5+Pia+Pid2+Pid3 | MR |
23P-40 | Guangdong | Pi2+Pi5+Pita | R |
23P-41 | Guangdong | Pia+Pid2+Pid3 | MR |
23P-42 | Guangdong | Pid3 | MS |
23P-43 | Guangdong | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-44 | Guangdong | Pi5+Pid2+Pid3 | MS |
23P-45 | Guangdong | Pi2+Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-46 | Guangdong | Pi2+Pi5+Pia | R |
23P-47 | Guangdong | Pid2+Pid3+Pita | MS |
23P-48 | Guangdong | Pid2+Pid3+Pita | MS |
23P-49 | Guangdong | Pi2+Pi5+Pia | MR |
23P-50 | Guangdong | Pi5+Pid2+Pid3 | MR |
23P-51 | Guangdong | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-52 | Guangdong | Pia+Pid3+pikh | R |
23P-53 | Guangdong | Pia+Pid3+pikh | R |
23P-54 | Guangdong | Pi2+Pi5+Pid2+Pid3 | MR |
23P-55 | Guangdong | Pi2+Pi5+Pia+Pita | R |
23P-56 | Guangdong | Pia+Pid2+Pid3+Pita | MR |
23P-57 | Guangdong | Pi2+Pi5+Pita | R |
23P-58 | Guangdong | Pi5+Pia+Pid2+Pid3 | MR |
23P-59 | Guangdong | Pi5+Pid2+Pid3 | MR |
23P-60 | Guangdong | Pi5+Pid2+Pid3+Pita | MR |
23P-61 | Guangdong | Pi2+Pi5+Pid2+Pid3 | R |
23P-62 | Guangdong | Pi5+Pid2+Pid3+Pita | MR |
23P-63 | Guangdong | Pia+Pid2+Pid3+Pita | MR |
23P-64 | Guangdong | Pi2+Pita | MR |
23P-65 | Guangdong | Pi5+Pid2+Pid3 | MS |
23P-66 | Guangdong | Pi5+Pid2+Pid3+Pita | MS |
23P-67 | Guangdong | Pi5+Pia+Pid2+Pid3 | S |
23P-68 | Guangdong | Pid2+Pid3+Pita | MS |
23P-69 | Guangdong | Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-70 | Guangdong | Pi2+Pi5+Pita | MR |
23P-71 | Guangdong | Pi2+Pi5+Pia+Pid2+Pid3+Pita | MR |
23P-72 | Guangdong | Pi2+Pi5+Pia+Pid2+Pid3+Pita | R |
23P-73 | Guangdong | Pi2+Pi5 | R |
23P-74 | Guangdong | Pid2+Pid3+Pita | R |
23P-75 | Guangdong | Pi2 | MS |
23P-76 | Guangdong | Pi5+Pid2+Pid3+Pita | MS |
23P-77 | Guangdong | Pi2+Pi5+Pid2+Pid3+Pita | MS |
23P-78 | Guangdong | Pi2+Pi5+Pita | R |
23P-79 | Guangdong | Pi5+Pid3 | MS |
23P-80 | Guangdong | Pi2+Pi5+Pia | MR |
23P-81 | Guangdong | Pi5+Pia+Pid2+Pid3+Pita | S |
23P-82 | Guangdong | Pia+Pid2+Pid3+Pita | S |
23P-83 | Guangdong | Pi2+Pi5+Pia+Pita | MS |
23P-84 | Guangdong | Pi5+Pia+Pid2+Pid3+Pita | MS |
23P-85 | Guangdong | Pi5+Pia+Pid2+Pid3 | S |
23P-86 | Guangdong | Pi5+Pia+Pid2+Pid3 | S |
23P-87 | Guangdong | Pi5+Pid3 | MS |
23P-88 | Guangdong | Pi2+Pita | MR |
23P-89 | Guangdong | Pi2+Pi5 | MR |
23P-90 | Guangdong | Pi2+Pita | MR |
23P-91 | Guangxi | Pid2+Pid3+Pita | MR |
The Number of Resistance Genes | The Number of Varieties | Resistance Frequency (%) | Resistance Grade of Varieties | |||
---|---|---|---|---|---|---|
R | MR | MS | S | |||
1 | 5 | 40 | 0 | 2 | 3 | 0 |
2 | 17 | 58.82 | 2 | 8 | 7 | 0 |
3 | 32 | 62.5 | 10 | 10 | 12 | 0 |
4 | 21 | 66.68 | 3 | 11 | 3 | 4 |
5 | 12 | 75 | 3 | 6 | 1 | 1 |
6 | 4 | 100 | 1 | 3 | 0 | 0 |
Gene Combination | Number of Varieties | Proportion (%) | Rice Blast Resistance Rate (%) |
---|---|---|---|
Pia+Pid2+Pid3 | 7 | 7.7 | 42.9 |
Pia+Pid3 | 1 | 1.1 | 0 |
Pid3+Pita | 1 | 1.1 | 100 |
Pia+Pid2+Pid3+Pigm | 1 | 1.1 | 100 |
Pia+Pid2+Pid3+Pigm+pikh | 1 | 1.1 | 100 |
Pia+Pid3+pikh | 3 | 3.3 | 66.7 |
Pi1+Pi5 | 1 | 1.1 | 0 |
Pi2+Pid2+Pid3 | 2 | 2.2 | 100 |
Pi2+Pi5+Pita | 6 | 6.6 | 83.3 |
Pi5+Pia+Pid2+Pid3+Pita | 9 | 9.9 | 77.8 |
Pia | 2 | 2.2 | 50 |
Pi5+Pi9+Pid2+Pid3 | 1 | 1.1 | 100 |
Pi5+Pi9+Pia+Pid2+Pid3 | 1 | 1.1 | 100 |
Pi5 | 1 | 1.1 | 100 |
Pi2+Pi5 | 5 | 5.5 | 80 |
Pid2+Pid3 | 1 | 1.1 | 0 |
Pi2+Pi5+Pia+Pid2+Pid3+Pita | 4 | 4.4 | 100 |
Pia+Pigm | 1 | 1.1 | 100 |
Pi5+Pia | 1 | 1.1 | 100 |
Pi5+Pid2+Pid3+Pita | 5 | 5.5 | 60 |
Pid2+Pid3+Pita | 6 | 6.6 | 50 |
Pi5+Pid2+Pid3 | 5 | 5.5 | 40 |
Pi5+Pid3 | 3 | 3.3 | 0 |
Pi5+Pia+Pid2+Pid3 | 6 | 6.6 | 50 |
Pi2+Pi5+Pia+Pita | 3 | 3.3 | 66.7 |
Pid3 | 1 | 1.1 | 0 |
Pi2+Pi5+Pia | 3 | 3.3 | 100 |
Pi2+Pi5+Pid2+Pid3 | 2 | 2.2 | 100 |
Pia+Pid2+Pid3+Pita | 3 | 3.3 | 33.3 |
Pi2+Pita | 3 | 3.3 | 100 |
Pi2 | 1 | 1.1 | 0 |
Pi2+Pi5+Pid2+Pid3+Pita | 1 | 1.1 | 0 |
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Tang, L.; Song, J.; Cui, Y.; Fan, H.; Wang, J. Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Varieties from South China. Plants 2024, 13, 2134. https://doi.org/10.3390/plants13152134
Tang L, Song J, Cui Y, Fan H, Wang J. Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Varieties from South China. Plants. 2024; 13(15):2134. https://doi.org/10.3390/plants13152134
Chicago/Turabian StyleTang, Liqun, Jian Song, Yongtao Cui, Honghuan Fan, and Jianjun Wang. 2024. "Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Varieties from South China" Plants 13, no. 15: 2134. https://doi.org/10.3390/plants13152134