Effect of CO2 Elevation on Tomato Gas Exchange, Root Morphology and Water Use Efficiency under Two N-Fertigation Levels
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Setup
2.2. Treatments
2.3. Measurements
2.3.1. Leaf Gas Exchange
2.3.2. Leaf Water Relation and ABA
2.3.3. Stomatal Aperture and Density
2.3.4. Root Morphology
2.3.5. Leaf Area and Plant Water Use Efficiency
2.3.6. Statistics Analysis
3. Results
3.1. Gas Exchange and WUE at Leaf Level
3.2. Plant Water Relation and Stomatal Morphology
3.3. Root Morphology
3.4. Leaf Area and Specific Leaf Area
3.5. Dry Matter and WUE at Plant Level
3.6. Principal Component Analysis (PCA) of Tomato Physiology, Growth and WUE
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Factors | Pn | gs | Tr | [ABA]leaf | WUEi | WUEn | Ψl | Kl | SA | SD |
---|---|---|---|---|---|---|---|---|---|---|
[CO2] | *** | ns | ns | *** | *** | *** | ns | ns | ns | ** |
[FN] | *** | *** | *** | * | ns | ns | ns | *** | ns | ns |
[N] | * | ** | ** | * | ** | * | * | *** | *** | ns |
[CO2] × [FN] | ns | * | * | ns | ns | ns | ns | * | ns | ns |
[CO2] × [N] | ns | ns | * | ns | ns | * | ns | * | ns | ns |
[FN] × [N] | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
[CO2] × [FN] × [N] | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
[CO2] | [FN] | [N] | RL (m) | RD (µm) | RSA (cm2) | RV (cm3) | SRL (m g−1) | SRSA (cm2 g−1) | RLD (cm cm−3) | RTD (mg cm−3) |
---|---|---|---|---|---|---|---|---|---|---|
a[CO2] | FIN | N1 | 223.53 ± 8.10 | 310.56 ± 5.12 | 2149.66 ± 57.74 | 16.56 ± 0.33 | 74.04 ± 2.52 a | 711.98 ± 15.64 a | 4.97 ± 0.18 | 182.40 ± 1.98 |
N2 | 191.88 ± 14.66 | 371.53 ± 13.45 | 2169.06 ± 127.97 | 19.70 ± 0.84 | 56.21 ± 3.18 d | 636.01 ± 23.28 b | 4.26 ± 0.33 | 173.19 ± 4.12 | ||
DIN | N1 | 211.36 ± 9.49 | 319.42 ± 4.67 | 2089.22 ± 72.19 | 16.53 ± 0.42 | 68.36 ± 3.37 abc | 675.92 ± 28.73 ab | 4.70 ± 0.21 | 187.80 ± 7.46 | |
N2 | 223.91 ± 10.31 | 355.86 ± 6.19 | 2434.21 ± 138.69 | 21.29 ± 1.52 | 61.79 ± 1.62 cd | 670.94 ± 20.93 ab | 4.98 ± 0.23 | 171.62 ± 7.58 | ||
e[CO2] | FIN | N1 | 219.20 ± 15.75 | 312.70 ± 4.29 | 2101.56 ± 176.43 | 16.17 ± 1.55 | 72.68 ± 1.92 ab | 695.18 ± 21.19 ab | 4.87 ± 0.35 | 188.24 ± 7.23 |
N2 | 231.50 ± 11.10 | 326.29 ± 4.52 | 2332.94 ± 118.96 | 18.90 ± 1.12 | 65.09 ± 2.35 bc | 655.54 ± 22.41 ab | 5.14 ± 0.25 | 189.28 ± 7.39 | ||
DIN | N1 | 206.29 ± 7.89 | 305.95 ± 2.52 | 1946.95 ± 76.25 | 14.70 ± 0.61 | 70.22 ± 1.65 ab | 662.68 ± 15.28 ab | 4.58 ± 0.18 | 200.28 ± 5.27 | |
N2 | 217.64 ± 14.69 | 331.67 ± 5.85 | 2240.50 ± 140.74 | 18.47 ± 1.15 | 55.08 ± 2.49 d | 566.91 ± 20.46 c | 4.84 ± 0.33 | 214.68 ± 6.99 | ||
ANOVA factor | ||||||||||
[CO2] | ns | *** | ns | ns | ns | ns | ns | *** | ||
[FN] | ns | ns | ns | ns | ns | ns | ns | * | ||
[N] | ns | *** | * | *** | *** | ** | ns | ns | ||
[CO2] × [FN] | ns | ns | ns | ns | ns | ns | ns | ns | ||
[CO2] × [N] | ns | ** | ns | ns | ns | ns | ns | * | ||
[FN] × [N] | ns | ns | ns | ns | ns | ns | ns | ns | ||
[CO2] × [FN] × [N] | ns | ns | ns | ns | * | * | ns | ns |
Factors | LA | SLA | LDM | SDM | RDM | TDM | PWU | WUEp |
---|---|---|---|---|---|---|---|---|
[CO2] | *** | *** | ns | *** | ns | ** | *** | *** |
[FN] | ns | ns | * | * | ns | ** | *** | *** |
[N] | *** | ns | *** | *** | *** | *** | *** | ** |
[CO2] × [FN] | * | ns | ns | ns | ns | ns | * | ns |
[CO2] × [N] | ns | ns | ns | * | ns | * | ns | *** |
[FN] × [N] | ** | * | ns | ns | ns | ns | ns | ns |
[CO2] × [FN] × [N] | *** | ** | ns | ns | ns | ns | ns | ns |
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Zhang, M.; Zhao, W.; Liu, C.; Xu, C.; Wei, G.; Cui, B.; Hou, J.; Wan, H.; Chen, Y.; Zhang, J.; et al. Effect of CO2 Elevation on Tomato Gas Exchange, Root Morphology and Water Use Efficiency under Two N-Fertigation Levels. Plants 2024, 13, 2373. https://doi.org/10.3390/plants13172373
Zhang M, Zhao W, Liu C, Xu C, Wei G, Cui B, Hou J, Wan H, Chen Y, Zhang J, et al. Effect of CO2 Elevation on Tomato Gas Exchange, Root Morphology and Water Use Efficiency under Two N-Fertigation Levels. Plants. 2024; 13(17):2373. https://doi.org/10.3390/plants13172373
Chicago/Turabian StyleZhang, Manyi, Wentong Zhao, Chunshuo Liu, Changtong Xu, Guiyu Wei, Bingjing Cui, Jingxiang Hou, Heng Wan, Yiting Chen, Jiarui Zhang, and et al. 2024. "Effect of CO2 Elevation on Tomato Gas Exchange, Root Morphology and Water Use Efficiency under Two N-Fertigation Levels" Plants 13, no. 17: 2373. https://doi.org/10.3390/plants13172373