Genome-Wide Identification of GH17s Family Genes and Biological Function Analysis of SlA6 in Tomato
Abstract
:1. Introduction
2. Results
2.1. Identification of GH17 Family Members and Analysis of Their Physicochemical Properties
2.2. Protein Motifs, Conserved Domains, and Gene Structure Analysis of GH17 Family Members
2.3. Evolutionary and Covariance Analyses of GH17 Family Members
2.4. Cis-Acting Elements Analysis of the Promoter Region of GH17 Family Members
2.5. Expression Pattern Analysis of the SlGH17s Family Members in Tomato Tissues
2.6. Expression Pattern Analysis of SlA6 during Tomato Flower Development and Response to Phytohormones
2.7. Knocking Out of SlA6 Affects Pollen and Fruit Development in Tomato
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of GH17 Family Members
4.3. Structural and Evolutionary Analyses of GH17 Family Members
4.4. Expression Pattern Analysis of GH17 Family Members
4.5. Expression Pattern Analysis of SlA6
4.6. Quantitative Real-Time PCR Analysis
4.7. Generating SlA6 Mutants Using CRISPR/Cas9 Technology
4.8. Identification of Transgenic Plant Phenotypes
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Name | ID | Protein Length/bp | Molecular Weight/Da | Theoretical pI | Instability Coefficient | Aliphatic Amino Acid Index | Hydrophilicity Coefficient |
---|---|---|---|---|---|---|---|
SlGH17-1 | Solyc01g008610 | 348 | 39.23 | 5.78 | 34.29 | 93.3 | −0.108 |
SlGH17-2 | Solyc01g008620 | 336 | 37.45 | 6.09 | 38.35 | 87.41 | −0.289 |
SlGH17-3 | Solyc01g059965 | 360 | 39.72 | 7.84 | 46.03 | 89.08 | −0.156 |
SlGH17-4 | Solyc01g060020 | 360 | 39.72 | 7.84 | 46.03 | 89.08 | −0.156 |
SlGH17-5 | Solyc01g109570 | 473 | 50.30 | 4.95 | 38.16 | 81.71 | −0.079 |
SlGH17-6 | Solyc02g070450 | 399 | 44.69 | 6.12 | 41.81 | 91.45 | 0.017 |
SlGH17-7 | Solyc02g080660 | 413 | 45.27 | 5.46 | 41.71 | 83.56 | −0.105 |
SlGH17-8 | Solyc02g086700 | 362 | 40.82 | 5.9 | 32.19 | 90.19 | −0.087 |
SlGH17-9 | Solyc03g025650 | 337 | 36.56 | 5 | 29.84 | 103.83 | 0.159 |
SlGH17-10 | Solyc03g058450 | 502 | 54.68 | 5.68 | 35.14 | 78.25 | −0.217 |
SlGH17-11 | Solyc03g082900 | 378 | 41.68 | 5.15 | 31.76 | 107.28 | 0.226 |
SlGH17-12 | Solyc04g007910 | 505 | 54.64 | 5.33 | 40.92 | 90.2 | 0.055 |
SlGH17-13 | Solyc04g011720 | 462 | 52.12 | 7.06 | 30.75 | 85.82 | −0.187 |
SlGH17-14 | Solyc04g011730 | 466 | 52.98 | 8.73 | 32.12 | 83.03 | −0.248 |
SlGH17-15 | Solyc04g015190 | 493 | 53.91 | 5.9 | 40.06 | 94.89 | 0.126 |
SlGH17-16 | Solyc04g051590 | 476 | 52.85 | 6.66 | 31.22 | 83.57 | −0.097 |
SlGH17-17 | Solyc05g006210 | 494 | 55.02 | 5.69 | 39.82 | 89.03 | −0.016 |
SlGH17-18 | Solyc05g015160 | 470 | 53.50 | 6.72 | 29.86 | 82.13 | −0.233 |
SlGH17-19 | Solyc05g015170 | 420 | 47.84 | 6.46 | 26.5 | 81.64 | −0.199 |
SlGH17-20 | Solyc05g025500 | 482 | 52.71 | 6.32 | 31.91 | 83.36 | −0.1 |
SlGH17-21 | Solyc05g054440 | 526 | 57.62 | 9.13 | 39.78 | 76.16 | −0.227 |
SlGH17-22 | Solyc06g073710 | 420 | 46.91 | 8.67 | 38.68 | 97.79 | −0.027 |
SlGH17-23 | Solyc06g076170 | 449 | 49.37 | 4.93 | 29.93 | 82.09 | −0.041 |
SlGH17-24 | Solyc07g005330 | 459 | 50.92 | 9.05 | 37.72 | 77.52 | −0.25 |
SlGH17-25 | Solyc07g008150 | 390 | 42.83 | 8.73 | 26.21 | 93.1 | −0.051 |
SlGH17-26 | Solyc07g017730 | 509 | 56.33 | 7.19 | 33.18 | 88.82 | −0.092 |
SlGH17-27 | Solyc07g049370 | 468 | 51.32 | 6.72 | 33.84 | 81.71 | −0.162 |
SlGH17-28 | Solyc08g074390 | 489 | 52.95 | 5.76 | 32.4 | 85.36 | −0.088 |
SlGH17-29 | Solyc08g083310 | 424 | 45.63 | 8.05 | 30.06 | 91.32 | 0.03 |
SlGH17-30 | Solyc09g057630 | 464 | 51.04 | 9.28 | 31.17 | 85.93 | −0.136 |
SlGH17-31 | Solyc10g078510 | 479 | 53.38 | 5.43 | 30.27 | 84.49 | −0.073 |
SlGH17-32 | Solyc10g079860 | 344 | 37.80 | 9.42 | 46.67 | 93.02 | −0.193 |
SlGH17-33 | Solyc11g012030 | 493 | 55.20 | 4.87 | 38.07 | 90.77 | −0.057 |
SlGH17-34 | Solyc11g065280 | 338 | 37.39 | 4.84 | 45.4 | 88.85 | −0.212 |
SlGH17-35 | Solyc11g068440 | 380 | 42.56 | 6.06 | 27.47 | 90.11 | −0.056 |
SlGH17-36 | Solyc11g071520 | 383 | 42.56 | 9.15 | 38.18 | 93.94 | 0.025 |
SlGH17-37 | Solyc11g072230 | 411 | 45.19 | 5.38 | 37.27 | 98.69 | 0.209 |
SlGH17-38 | Solyc12g008580 | 496 | 53.95 | 5.91 | 34.82 | 79.31 | −0.073 |
SlGH17-39 | Solyc12g014420 | 480 | 51.46 | 5.59 | 39.02 | 82.9 | −0.149 |
SlGH17-40 | Solyc12g019890 | 475 | 52.35 | 4.89 | 32.02 | 81.96 | −0.052 |
SlGH17-41 | Solyc12g040860 | 456 | 50.39 | 6 | 29.34 | 84.87 | −0.134 |
SlGH17-42 | Solyc12g055840 | 422 | 45.54 | 6.52 | 32.48 | 88.48 | 0.006 |
SlGH17-43/SlA6 | Solyc12g098560 | 459 | 51.89 | 5.09 | 39.87 | 85.62 | −0.22 |
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Chen, D.; Zeng, Z.; Yu, C.; Hu, H.; Lin, Y.; Wu, C.; Yang, Y.; Zhong, Q.; Zhang, X.; Huang, C.; et al. Genome-Wide Identification of GH17s Family Genes and Biological Function Analysis of SlA6 in Tomato. Plants 2024, 13, 2443. https://doi.org/10.3390/plants13172443
Chen D, Zeng Z, Yu C, Hu H, Lin Y, Wu C, Yang Y, Zhong Q, Zhang X, Huang C, et al. Genome-Wide Identification of GH17s Family Genes and Biological Function Analysis of SlA6 in Tomato. Plants. 2024; 13(17):2443. https://doi.org/10.3390/plants13172443
Chicago/Turabian StyleChen, Da, Zaohai Zeng, Canye Yu, Huimin Hu, Yuxiang Lin, Caiyu Wu, Yinghua Yang, Qiuxiang Zhong, Xinyue Zhang, Caihong Huang, and et al. 2024. "Genome-Wide Identification of GH17s Family Genes and Biological Function Analysis of SlA6 in Tomato" Plants 13, no. 17: 2443. https://doi.org/10.3390/plants13172443