Ergothioneine Improves Seed Yield and Flower Number through FLOWERING LOCUS T Gene Expression in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Effect of EGT on Seed Yield of A. thaliana
2.2. Growth of A. thaliana at the Reproductive Growth Stage
2.3. Effect of EGT Application at the Reproductive Growth Stage on Seed Yield
2.4. Effects of EGT on Vegetative Organs of A. thaliana at the Vegetative Growth Stage
2.5. Effect of EGT on Flower Number
2.6. Effect of EGT on Gene Expression of Flowering Hormone
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Application Conditions
4.3. Growth Parameters
4.4. RNA Extraction and cDNA Synthesiss
4.5. RT-qPCR Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Locus | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) |
---|---|---|---|
FLOWERING LOCUS T | AT1G65480 | CTACAACTGGAACAACCTTTGGC | CGAGTGTTGAAGTTCTGGCG |
UBC21 | AT5G25760 | TCCTCTTAACTGCGACTCAGG | GCGAGGCGTGTATACATTTG |
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Koshiyama, T.; Higashiyama, Y.; Mochizuki, I.; Yamada, T.; Kanekatsu, M. Ergothioneine Improves Seed Yield and Flower Number through FLOWERING LOCUS T Gene Expression in Arabidopsis thaliana. Plants 2024, 13, 2487. https://doi.org/10.3390/plants13172487
Koshiyama T, Higashiyama Y, Mochizuki I, Yamada T, Kanekatsu M. Ergothioneine Improves Seed Yield and Flower Number through FLOWERING LOCUS T Gene Expression in Arabidopsis thaliana. Plants. 2024; 13(17):2487. https://doi.org/10.3390/plants13172487
Chicago/Turabian StyleKoshiyama, Tatsuyuki, Yukihiro Higashiyama, Izumi Mochizuki, Tetsuya Yamada, and Motoki Kanekatsu. 2024. "Ergothioneine Improves Seed Yield and Flower Number through FLOWERING LOCUS T Gene Expression in Arabidopsis thaliana" Plants 13, no. 17: 2487. https://doi.org/10.3390/plants13172487